Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"Brain slice"”.
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Quantifying effects of aging across structures of the mouse auditory system, with in-vitro brain slice preparations
… loss may be primary, hearing requires central brain structures, not just the ear. These central structures are themselves sensitive to the process of aging and their attempts to compensate for peripheral loss combined with their own changes are not always successful in maintaining proper …
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The Murine Brain Slice as a Model for Investigation of Environmental Toxin Involvement in the Etiology of Parkinson's Disease
… cyclodienes are environmentally persistent, and brain residues of these compounds are correlated with the occurrence of PD. Cyclodiene mode of action has been attributed to two mechanisms: 1) facilitation of neurotransmitter release (with specificity for release of dopamine) and 2) antagonism of …
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Investigating gene expression differences in acute and organotypic mouse hippocampal brain slices as models for studying the brain
Organotypic brain slice cultures (OBSCs) are widely used to study neural circuit function, but their molecular and cellular composition relative to acute brain slices remains poorly characterized. Here, using single-nucleus RNA sequencing, I performed a comprehensive comparison of gene expression …
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Functional Peptidomics: Combining Discovery-Based Mass Spectrometry and Neurophysiology to Explore Communication of Phase -Resetting Cues in the Rat Suprachiasmatic Nucleus
… release properties of peptides from the SCN brain slice provides new insights on a complex phenomenon phenomenon. The identification and characterization of little SAAS serves as an example of the facets of SCN physiology left to be discovered. Utilization of peptidomics strategies will …
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Cholinergic regulation of a mammalian circadian clock
… rhythm in neuronal activity in a hypothalamic brain slice. Using this in vitro preparation, we investigated the direct regulation of the SCN circadian pacemaker by cholinergics.
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Use of an Atg16l1 mouse model to study the role played by autophagy in neuroprotection
… no effect on virus proliferation in organotypic brain slice cultures while viral nsP1 and capsid expression was reduced in Atg16l1 KO embryonic fibroblast cultures. Semliki Forest Virus markers did not colocalize with autophagosomes in autophagy competent cells. While Semliki Forest Virus causes …
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Investigation of the Signaling Mechanisms of the Pineal Hormone Melatonin in the in Vitro Rat Suprachiasmatic Nucleus
… nucleus (SCN) of the hypothalamus in the brain. The SCN controls production of the pineal hormone, melatonin, thereby restricting its release to the nighttime. The SCN, in turn, expresses high-affinity receptors for melatonin, and melatonin can feed back to the SCN to regulate its timing. …
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Exploring the effects of classical immune activation on circuit excitability and cell viability in the mouse brain
… development. Using the hippocampal organotypic brain slice culture model in mouse brains, I investigated the effects of classical immune activation on circuit excitability and cell viability. To initiate inflammation, I administered lipopolysaccharide (LPS), an endotoxin derived from …
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Establishing in vitro models of neuroinflammation to investigate neuroimmune responses in neurocysticercosis
… of NCC is that viable larvae can exist in the brain for extended periods with no symptomatology, but when they die, clinical symptoms develop. Clinical evidence suggests that the hallmark of symptomatic NCC is neuroinflammation; however, the neuroinflammatory mechanisms underlying this disease …
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Fatty acid ethanolamides pinpoint nicotinic receptors and modulate neuronal excitability through nuclear receptor PPARα
… their mechanism of action in an in vitro brain slice preparation. 2) To determine whether there is an interaction between PPARα and nAChRs in VTA DA cells: particularly, to characterize the postsynaptic effects of PPARα modulation on VTA DA neurons, and to examine the contribution of …
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Exploring molecular and cellular mechanisms underlying seizures in neurocysticercosis
… by combining mouse hippocampal organotypic brain slice cultures with various preparations of a model parasite, Taenia crassiceps. Utilising these models, I first explored, using patch clamp and local field potential electrophysiology, how Taenia larval extracts directly affect neuronal …
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The Giant Walkthrough Brain Project: Interactive Medical Education for the Individual, Classroom, and Beyond
… by Joseph Bogen's idea of the Giant Walkthrough Brain, this thesis discusses the creation of three innovative methods that allow us to visualize and explore human brain anatomy. First is the Giant Walkthrough Brain Show: a live virtual tour of a 3D brain museum in collaboration with science …
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Hybrid Brain-Tissue Based Model to Study the Role of Infiltrating Monocytes in Late Stage Huntington's Disease
… on the hypothesis that monocytes infiltrate the brain of late stage HD patients and drive neuropathogenesis. To test this hypothesis, I developed a novel hybrid brain-slice experimental model in which human monocytes (MO) isolated from HD and non-disease control patients (CTRL) are engrafted into …
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The role of light signaling on astrocytic morphological plasticity in the adult male rat suprachiasmatic nucleus
… The SCN then relays this information to the brain and the rest of the body through synaptic signaling and indirectly through circadian regulation of hormonal signaling. The SCN is unique in that in the absence of external signaling, circadian rhythms will persist. This is accomplished through …
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Understanding the role of striosomes in learning a decision-making task
… behavior recordings, and histological brain slice images of mice, I find that: 1) the activity of spiny projection neurons (SPNs) in striosomes correlate with learning ability; 2) an animal's attentiveness to the task is critical for learning; 3) anatomical circuit disassembly, …
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Mechanisms of DNA double strand break-mediated neurotoxicity in neurodegenerative disease
… transcriptomics reveal that regions of CK-p25 brain tissue dense with DSB-bearing neurons harbor signatures of inflammatory microglia, which is ameliorated by NFκB knock down in neurons. Inhibition of NFκB in DSB-bearing neurons also reduces microglia activation in organotypic mouse brain slice …
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Effects of weak electric fields on long-term synaptic plasticity
… to the scalp with the goal of stimulating the brain. There is tremendous interest in the use of tDCS for treating brain disorders and improving brain function. However, the effects of tDCS have been highly variable across studies, leading to a debate over its efficacy. A major challenge is …
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A Novel Link Between Oxidative Stress Proteins and Estrogen Receptor Alpha-Mediated Gene Expression
… MCF-7 cells. Additional characterization using brain slice cultures suggests a protective role for E2 and SOD1 in the cerebral cortex. These combined studies highlight a novel link between oxidative stress proteins and ERalpha-mediated gene expression in target tissues.
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