Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"Brain Slices"”.
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A novel device for interrogation of neuron connectivity in acute brain slices
… scale of inter-neuron communication in the human brain presents an immense obstacle to understanding the mechanisms of information processing and how changes to neuronal connectivity are associated with pathological states. Neuron interactions underlie functional roles of local brain networks, for …
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Control of Sexually Dimorphic Brain Pathway Development in Cultured Zebra Finch Brain Slices
… describes the development of a zebra finch brain slice culture system, and its application to identify the signals controlling formation of the HVC-RA pathway. Using a stationary culture method and a semidefined medium, metabolically active slices from adult brains were maintained for six …
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Investigating gene expression differences in acute and organotypic mouse hippocampal brain slices as models for studying the brain
Organotypic brain slice cultures (OBSCs) are widely used to study neural circuit function, but their molecular and cellular composition relative to acute brain slices remains poorly characterized. Here, using single-nucleus RNA sequencing, I performed a comprehensive comparison of gene expression …
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The Role of Temperature in the Preparation and Viability of Rodent Brain Slices, and Propensity to Evoke Spreading Depolarization
… propagates across the higher grey matter of the brain and is the primary contributor to acute neuronal injury. Within minutes of the brain being deprived of oxygen and glucose (OGD), Na+/K+ pumps on neuronal membranes fail and membrane potential is lost, resulting in SD. Experimentally, SD can be …
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Towards mapping spatial transcriptome of an entire vertebrate brain
Both the brain’s substantial complexity and technical challenges in monitoring and manipulating brains present challenges for understanding this essential organ. Zebrafish, for their modest brain size and transparency in the larval stage, serve as a model organism for whole-brain in vivo imaging …
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Exploring the effects of classical immune activation on circuit excitability and cell viability in the mouse brain
… development. Using the hippocampal organotypic brain slice culture model in mouse brains, I investigated the effects of classical immune activation on circuit excitability and cell viability. To initiate inflammation, I administered lipopolysaccharide (LPS), an endotoxin derived from …
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Stretch induced hyperexcitability of mice callosal pathway
… Previous studies on synaptic physiology in brain slices have traditionally been focused on biochemical processes. Here, we demonstrate with experiments on mouse brain slices that central nervous system plasticity is also sensitive to mechanical stretch. This is important, given the host of …
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The neuroimmune response to cryptococcal infection
… (CM) is a fatal fungal infection of the brain that is responsible for up to 20% of all AIDS-related deaths globally, 75% of which are from Sub-Saharan Africa. CM is characterised by debilitating neurological damage often resulting in death or serious longterm sequelae even after receiving …
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Tumor-assoziierte Mikroglia und Makrophagen modellieren durch GPNMB das Tumorwachstum und die Immunzellinfiltration in malignen Gliomen
… distinct murine glioma cell lines in organotypic brain slices from GPNMB-KO and control mice. Using published data bases of human glioma, the elevated levels in TAMs could be confirmed and the GPNMB expression correlated with a poorer survival.
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The effects of chronic intermittent ethanol exposure on withdrawal related behaviors, dopamine terminal function and kappa opioid system sensitivity in the nucleus accumbens core
… with fast scan cyclic voltammetry in brain slices showed reduced dopamine release, augmented uptake and increased sensitivity of KORs and D3Rs following five weeks of CIE exposure. A subsequent examination of interactions between KORs and dopamine autoreceptors showed that D3Rs and …
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Age-dependent effects of chronic amphetamine on prelimbic-nucleus accumbens circuit function and goal-directed behavior
… 3). In Experiment 1, field potentials in brain slices from male naive rats were assessed to test the effect of age on the expression of this LTD. The role of GABA and dopamine receptors in the HFS-induced LTD was investigated using respective agonist or antagonist. In Experiment 2, brain …
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Investigating neural responses in models of neurocysticercosis
… than the rest of the world due to high levels of brain infections by larvae of the pig cestode Taenia solium, a condition termed neurocysticercosis. Despite the large nature of the problem, little is known about how neurocysticercosis modulates neuronal responses to result in the development of …
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Exploring molecular and cellular mechanisms underlying seizures in neurocysticercosis
… by combining mouse hippocampal organotypic brain slice cultures with various preparations of a model parasite, Taenia crassiceps. Utilising these models, I first explored, using patch clamp and local field potential electrophysiology, how Taenia larval extracts directly affect neuronal …
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Hybrid Brain-Tissue Based Model to Study the Role of Infiltrating Monocytes in Late Stage Huntington's Disease
… on the hypothesis that monocytes infiltrate the brain of late stage HD patients and drive neuropathogenesis. To test this hypothesis, I developed a novel hybrid brain-slice experimental model in which human monocytes (MO) isolated from HD and non-disease control patients (CTRL) are engrafted into …
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Spontaneous activity in the mouse visual cortical slice: biophysical characterization and pathophysiology
… form of pathophysiology that arises in brain slices prepared from a mouse model of FXS, the Fmr1-knockout (KO) mouse. Relative to wildtype (WT) slices, Fmr1-KO visual cortical slices exhibit increased spiking activity in layer 5. Critically, this hyperactivity phenotype is rapidly …
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THE EFFECT OF SPILLOVER ONTO IONOTROPIC GLUTAMATE RECEPTORS AT THE SCHAFFER-CA1 SYNAPSE IN HIPPOCAMPAL SLICE
… is the major excitatory neurotransmitter in the brain and is crucial for processes such as learning and memory. Due its importance as a signaling molecule, the extracellular glutamate concentration is tightly regulated, largely by the excitatory amino-acid transporters (EAATs). In these studies, …
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THE EFFECT OF SPILLOVER ONTO IONOTROPIC GLUTAMATE RECEPTORS AT THE SCHAFFER-CA1 SYNAPSE IN HIPPOCAMPAL SLICE
… is the major excitatory neurotransmitter in the brain and is crucial for processes such as learning and memory. Due its importance as a signaling molecule, the extracellular glutamate concentration is tightly regulated, largely by the excitatory amino-acid transporters (EAATs). In these studies, …
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Adenosine Production Is Essential for Closing the Critical Period of Cortical Plasticity
… as highly organized systems in the adult brain for effective adaptation to the environment. At the cortical level, this organization is referred to as cortical maps. The establishment of cortical maps begins with early life experiences during the critical period, a brief period during …
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The Role of Atypical PKC in the Plasticity of the Circadian Clock in the Rat Suprachiasmatic Nucleus
… increased significantly by GLU treatment to SCN brain slices at CT 14 in the early night. Disruption of aPKC phosphotransferase activity completely blocks GLU-induced phase delays in the early night, but not phase advances in the late night, demonstrating that aPKC is essential for GLU-induced …
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