Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Boyden chamber"”.
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A Microsystems Approach for Drug Assessment
… approaches for this purpose: a microengineered Boyden chamber, and a dielectrophoresis-based platform for cell characterization and drug assessment. Using these methods, we characterize the drug response of breast cancer, which is the second most common type of cancer among US women. The …
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PTEN loss induces epithelial-mesenchymal transition in human colon cancer cells
… Invasiveness was measured using a modified Boyden chamber assay and migration was assessed using a scratch assay. Results. PTEN knockdown increases the invasion and migration of CRC cells and the addition of medium containing TNF-á further enhanced the migration and invasion. PTEN knockdown …
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Untersuchungen zum c-met/HGF-System an humanen Mesothelzellen in vitro
… than cells without HGF-stimulation. A modified Boyden-chamber-system indicated a chemotactical effect of HGF on mesothelial cells relative to concentration. As the plasmin activation appears to be a crucial factor in cellular migration, the question arose, as to whether this migration of …
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Surface Engineered Novel Patterned Polymers to Remove Pathogenic Biofilms from Human Skin. Effective Removal of Antimicrobial Resistant Bacteria from Chronic Wounds
… Bacterial infection in co-culture using the Boyden chamber assay increased fibroblast viability in the presence of PDMS (*p<0.05). PT 10% demonstrated superior biofilm transfer from epidermis (*p<0.05), in comparison to all other analysed polymers. In summary, the unique topography of PDMS …
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Role of N-terminal residues of CCL19 and CCL21 in binding and activation of CCR7
… vitro assays such as calcium flux, trans-well (Boyden chamber), and Western blotting. We also carried out computational studies to better understand and predict the activity of these peptides. Our results demonstrate that some of these peptides are indeed capable of acting as agonists or …
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Die Charakterisierung der differentiellen Genexpression invasiver und nicht-invasiver humaner glatter Gefäßmuskelzellen
… This potential was tested in a modified Boyden Chamber. The phenotypes act as a model of non-invasive smooth muscle cells in healthy arterial wall respectively smooth muscle cells capable of proliferation and migration after PTCA. Using a cDNA array system 558 cardiovascular-associated …
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Invasionshemmung von Magentumorzellen nach Behandlung mit Phospholipiden in vitro
… We tested the migration of the tumor cells at a boyden chamber assay. Therefore we pretreted the tumor cells in three different test arrangements with two different PL concentrations. The different test arrangement should imitate the peritoneal cavity with its extracellular matrix. After 24 hours …
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Der Einfluss von N-Cadherin auf die Migration glatter Gefäßmuskelzellen im Rahmen der Restenoseentstehung
… Quantification of cell migration was detected by Boyden chamber assays. Regulation of RhoA GTPase was tested by assessment of RhoA activity. Results: The analysis of N-cadherin expression in vivo by a porcine restenosis model revealed downregulation in the neointima after 14 days. After 28 days, …
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SENSITIZATION OF HUMAN OSTEOSARCOMA CELL LINE 143B WITH CALCITRIOL FOR CISPLATIN THERAPY
… effects were evaluated by MTS assay. Boyden chamber assay was used for migration/invasion studies. MMP activity was measured using Sensolyte kit and real time PCR for the detecting the expression of MMP-1. Results: Calcitriol does not sensitize the 143B-P and 143B-MM OS cells for …
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A Method to Improve Cartilage Integration
… at concentrations of 25 ng/mL in modified Boyden chamber experiments. However, in bovine articular cartilage samples that were pre-treated with collagenase (0.6% for 10 min), supplementation with 25 ng/mL of either PDGF-bb or bFGF had no apparent effect on the ZCD relative to samples …
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Μελέτη της αντι-αγγειογενετικής δράσης των συνθετικών πεπτιδίων HB-19 και N6L
… στο in vitro σύστημα μελέτης της μετανάστευσης Boyden chamber. Το πεπτίδιο HB-19 αναστέλλει την έκφραση διαφόρων αγγειογενετικών αυξητικών παραγόντων όπως ο FGF, VEGF και των υποδοχέων τους σε επίπεδο mRNA. Το HB-19, όπως και το Ν6L επηρεάζουν την έκφραση της μεταλλοπρωτεϊνάσης MMP-2 τόσο σε …
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Anti-cancer effects and mechanism of actions of aspirin-like drugs in the treatment of gliomas
… Finally, PN517 reduced migration in both the Boyden chamber and scratch assays, but did not inhibit invasion. In conclusion, these data support the further development of PN517 as a novel therapeutic drug for the treatment of glioma.
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In Vitro Assessment of Bone and Cartilage Stimulating Peptides
… assay, an Alizarin Red S staining assay and a Boyden Chamber assay, respectively. Osteoprogenitor cells examined included MC3T3-E1 preosteoblasts, human bone marrow-derived mesenchymal stem cells (hBM-MSCs), human periosteum-derived stem cells (hPDSCs), and rabbit periosteum-derived stem cells …
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Development of Delivery Strategy for Adipose-Derived Stem Cells in the Treatment of Myocardial Infarction
… factor (HGF) was analyzed using a modified Boyden chamber assay. The results demonstrated that SDF-1α and HGF, at 20, 50, and 100 ng/mL elicited significant migratory responses under normoxic (21%) and hypoxic (5%) culture conditions. RT-PCR analysis was conducted to assess the expression of …
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Die Rolle der Chemokinrezeptoren CXCR3 und CXCR7 bei den tumorogenen Einflüssen von CXCL11
… Proliferation verglichen werden. Mit Hilfe des Boyden-Chamber-Assay konnte gezeigt werden, dass CXCL11 die Migration aller Tumorzelllinien stimuliert. Die chemotaktische Antwort der einzelnen Tumorzelllinien auf CXCL11 zeigte dabei eine unterschiedliche Sensitivität für den spezifischen …
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Discovery of prognostic biomarkers from the prostate cancer membrane proteome using bioorthogonal chemistry
… the study was confirmed in the scratch wound and Boyden chamber assays and by demonstrating a correlation with increased expression of vimentin, loss of PTEN expression, and decreased E-cadherin expression. Collectively, the data validated the panels as suitable in vitro models of prostate and …
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TIMP-1: Zelluläre Funktion und Beeinflussung der Genexpression von Matrix-Metalloproteinasen in humanen Hepatomzellen
… hepatoma cells was investigated in the Boyden chamber. The TIMP-1 overexpression seems to promote the migration. Presumably this is due to an increased secretion of MMP (MMP-2 and MMP-9) in TIMP-1 overexpression cells, as via MMP inhibition the migration of these cells could be reduced …
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TRIB3 as an integrator of metabolic stresses: Role in lung cancer progression
… was evaluated using MTS and trypan blue assays. Boyden chamber assay was used to assess the cell migration while cell cycle phases were determined using flow cytometry. Heat-map analysis was performed to assess the changes in the expressions of genes in cell cycle progression and apoptosis. In …
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PEPTIDE ENGINEERING FOR DEVELOPMENT OF ANTIMICROBIALS AGAINST Mannheimia haemolytica
… peptides on bovine neutrophils was studied using Boyden chamber. Peripheral blood neutrophils isolated from normal healthy cattle showed chemotaxis towards HBD3 and 20AA peptides (P<0.05) but not towards 28AA analogue. Co-incubation of neutrophils with any of the peptides did not affect their …
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Collective Behavior of Drosophila Melanogaster Neural Progenitor and Imaginal Disc Cells within Controlled Microenvironments
… adhere and grew in suspension on LM. Utilizing Boyden Chamber Assays, D17 cells showed significant migration toward brain-derived neurotrophic factor (BDNF), concentration-dependent migration toward Insulin (In), and no significant migration toward FGF8. Furthermore, D17 cells showed high …
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