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Showing 1 to 13 of 13 for “"Bovine parvovirus"”.
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Replication of bovine parvovirus
Bovine parvovirus (BPV) is a small icosahedral virus containing single stranded DNA and belongs to the group of nondefective parvoviruses. Research on this virus group has revealed that viral replication occurs in cycling cells only, and evidence exists that the required factor provided by cycling …
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Infectivity and Epidemiological Studies on Bovine Parvovirus
Bovine parvoviruses (BPV) are widespread in the cattle population and associated with disease of the reproductive and possibly with the respiratory tract. The virus has been recovered from cattle with clinical signs of respiratory disease. Four established cell lines (bovine turbinates (BT), Madin …
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Isolation and characterization of bovine parvovirus DNA
The best characterized of the autonomous parvoviruses are those of rodent origin. The purpose of this study was to examine the physicochemical characteristics of the genome of an autonomous parvovirus of non-rodent origin, bovine parvovirus (BPV). BPV was isolated from infected cells by …
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Electron microscopy of bovine Parvovirus DNA replication intermediates
… DNA species have been isolated from autonomous parvovirus-infected cells that could·not be demonstrated in mock-infected cells. The DNA species were termed replicative forms and intermediates. The purpose of this study was to isolate and characterize the replicative forms from bovine parvovirus …
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Bovine parvovirus and bovine enterovirus in mixed infections
Bovine fetal spleen cells synchronized with 2mM hydroxyurea were infected with bovine parvovirus and bovine enterovirus in order to study the events occurring when DNA and RNA viruses mixedly infect single cells. The objectives of this research were threefold. First, to determine the effects of …
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Transcription and encapsidation in parvoviruses LuIII and bovine parvovirus
The termini of the autonomous parvovirus LuIII, which encapsulates plus and minus DNA strands equally, were cloned and sequenced. The left and right termini of LuIII differ in nucleotide sequence and these termini can assume T- and U-shaped intra-strand base-paired structures, respectively. The …
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Molecular cloning and analysis of the genome of bovine parvovirus
The genome of bovine parvovirus (BPV) has been cloned by blunt end ligation of double-stranded virion DNA into the plasmid pUC8. The resulting genomic clones were infectious after transfection into bovine fetal lung (BFL) cells. Sequencing of the plasmids demonstrated that deletions were common at …
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Cis and trans signals for the replication of bovine parvovirus
… for the S-phase factor required for autonomous parvovirus replication. The BPV ori thus appears to function by binding both cellular and viral proteins for the initiation of DNA synthesis from the hairpinned termini. The comparison of the BPV and LPV genome sequence suggest that the genomic …
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Assessment of Cell Death Parameters in Bovine Parvovirus-Infected EBTr Cells
Bovine parvovirus (BPV) is a helper-independent parvovirus. It has a small icosahedral capsid with a single stranded DNA genome. It is a highly stable virus with a narrow host range. It causes acute gastroenteritis in calves. It is considered to be a cytolytic virus because it kills the host cells. …
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The association of bovine parvovirus DNA and proteins with the nuclear matrix of infected cells
Bovine parvovirus DNA is associated with the nuclear matrix of infected bovine fetal lung cells as shown by Southern blot analysis of matrix DNA isolated by two procedures differing in the order of exposure of detergent-treated nuclei to high salt conditions and DNase I. Protein analysis of the two …
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Cellular factors and viral elements for parvovirus replication
Autonomous parvoviruses, such as bovine parvovirus (BPV), need a factor present at the S-phase of the cell cycle for a productive infection, while dependent parvoviruses, the adenoassociated viruses (AAVs), require a helper virus to complete an infectious cycle. However, AAV can replicate …
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Analysis of factors that affect parvovirus expression
… from the promoter located at map unit 4 of the bovine parvovirus (BPV) genome were determined. The autonomous parvoviruses, of which BPV is a member, contain two transcriptional units with promoters active in temporal order during infection. BPV proteins also appear in the same temporal order; …