Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Botulinum Neurotoxin"”.
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Engineering functional recombinant proteins based on antibody domains and fragments of C. botulinum neurotoxin
… and single domain antibodies. Recombinant botulinum neurotoxin fragments derived from serotype A, B, C and D are selected as backbones for an investigation into the feasibility and properties of novel recombinant targeted multidomain proteins. Single chain Fv’s consist of a VH region of …
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Thermodynamic Evidence That Ganglioside-Mediated Insertion Of Botulinum A Into The Cholinergic Nerve Ending May Precede Endocytosis And Acidification: A Langmuir Film Study
Botulinum Neurotoxin (BoNT) is one of the most potent toxins known to human kind. The Atomic Force Microscope (AFM) was employed to investigate the conditions under which BoNT type A heavy chain would bind and/or insert into mica supported dipalmitoylphosphatidylcholine (DPPC) lipid bilayers. As an …
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DEVELOPMENT OF SURFACE BASED PLATFORMS FOR BIOMOLECULAR RECOGNITION AND PROTEASE ASSAYS
… from our main focus of studies, the Clostridium botulinum Neurotoxin type A Light Chain (BoNTA/LC) metalloprotease. Supplementary studies include assays performed involving the Bacillus anthracis Lethal factor metalloprotease. In the work described we have prepared active recombinant protease …
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DEVELOPMENT AND KINETIC MODELING OF MULTIPLEX MICROSPHERE ASSAYS FOR HIGH-THROUGHPUT DISCOVERY OF PROTEASE ACTIVE SMALL MOLECULE COMPOUNDS
… anthracis lethal factor protease (LF) and the Botulinum neurotoxin type A & F light chain proteases (BoNT/A & F LC). In order to select the efficient inhibitors, a chemical library of 350,000 compounds was screened with the HyperCyt® high-throughput flow cytometry system, which can screen …
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Palmitoylation and Oxidation of the Cysteine Rich Region of SNAP-25 and their Effects on Protein Interactions
… Also, oxidized SNAP-25 is not susceptible to Botulinum Neurotoxin E. The effects of oxidation and palmitoylation on the protein interactions of SNAP-25 may shed light on its role in the SNARE complex and membrane fusion.</p>
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Evaluation of Global Muscle Innervation Using Multi-Channel Surface Electromyography
… trial showed higher treatment effectiveness of botulinum neurotoxin in imaging-guided injection group. IZ imaging using electrically elicited compound muscle action potential can greatly reduce computational demand. Furthermore, we have for the first time, provided the feasibility of estimating …
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Protein engineering of botulinum toxins with enhanced ganglioside binding capacity A biophysical and computational approach
Botulinum toxins (BoNTs) comprise a family of extremely potent neurotoxins, which have been harnessed as muscle relaxants for the treatment of a wide variety of debilitating diseases, particularly for the treatment of movement disorders. BoNT entry into neurons leads to destructive cleavage of …
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The establishment and characterization of an improved cell-free assay for exocytosis in neuroendocrine PC12 cells
… Plasmamembran einhergeht. Durch Inkubation mit Botulinum Neurotoxin C1, einer Protease die spezifisch Syntaxin 1 schneidet, konnte diese Bindung unterbunden werden. Sowohl die Bindung an die Membran, als auch die Inhibition der Exozytose wurden durch die Gegenwart von NSF verhindert. Für eine …
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Neurological Mechanisms Underlying Rotator Cuff Skeletal Muscle Pathophysiology and Implications for Therapy
Introduction: Rotator cuff tears are a common cause of upper-extremity disability. For chronic, full-thickness, rotator cuff tears, repair surgery can be technically challenging due to large gap distances and increased stiffness of the muscle-tendon unit. In contrast, acute rotator cuff tears are …
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Exploring new chemical entities from traditional medicine: Docking, synthesis and specific bioactivities
… aim is to identify active phytochemicals against botulinum neurotoxin A (BoNT/A), and diabetes from Ayurveda and TCM, respectively, by using in silico, in vitro and in vivo approaches. In our second aim, we wanted to enantioselectively synthesize scalable quantities of phytoestrogenic isoflavans …