Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"Bone cells"”.
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Proto-oncogene Regulation by Growth Factors in Bone Cells
<p>Previous studies have shown that bone cells in culture produce a number of growth factors that are important in bone regulation. Chick and mouse primary calvarial cultures consist of a mixture of cell types that make it difficult to interpret the results from these model systems. In contrast, …
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Organosilicate nanoparticles as gene delivery vehicles for bone cells
… proliferation. The metabolic response of these cells after particle ingestion was also characterized in order to ensure that the osteoblasts retained their phenotype. The expression of various proteins involved in bone formation, such as alkaline phosphatase, osteocalcin, osteopontin and …
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Retinoic Acid Regulation of Thyroid Hormone Action in Bone Cells
… hormones interact with each other to regulate bone functions remains to be established. We investigated how thyroid hormone and retinoic acid interact to regulate cells involved in endochondral bone formation. We demonstrate that thyroid hormone treatment stimulates differentiation of ATDC5 …
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Delineating the metastatic process in osteosarcoma; the role of bone cells, the cell environment and autophagy
… CXCR4 (2-fold; p≤0.001) compared to monolayer cells. We have termed these spheroids with enhanced migratory capabilities ‘Migratory Bodies’ (MBs). We subsequently assessed the effect of OC-conditioned media on MB formation, where a significant reduction in MB formation in HOS-143B cells …
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Tyrosyl Protein Phosphorylation in Human Bone Cells : Its Potential Role in Mediating Fluoride Induced Osteoblast Proliferation
… relationships similar to fluoride induced bone cell mitogenesis (i.e. 220, 34 and 32kD), b) Several other bone cell mitogens stimulated tyrosyl phosphorylation of similar molecular weight proteins as fluoride, suggesting that these proteins are important mediators of bone cell proliferation …
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Molecular Mechanism of the Stimulation of Alkaline Phosphatase Activity in Human Bone Cells by 1,25(OH)2 D3
… The human osteosarcoma cell line (TE85 cells) expressed a skeletal alkaline phosphatase activity (an accepted bone cell differentiation marker) which was stimulated by 1,25(OH)<sub>2</sub>D<sub>3</sub> (a potent differentiating agent), under serum-free conditions in a dose-dependent, …
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Mechanism of Mitogenic Action of Aluminum Ion on Bone Cells : Potential Involvement of the Insulin-Like Growth Factor Regulatory System
… differentiation marker) in osteoblast-like cells of chicken and human origin. Although biphasic, dose-dependent stimulations were highly reproducible, the maximal stimulatory dose varied among different experiments. Mitogenic doses of aluminum ion also stimulated collagen synthesis in …
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Characterization and Implementation of Low Intensity Pulsed Ultrasound as a Tool to Apply Physical Load to Scaffolds and Bone Cells for Fracture Repair
<p>One current challenge in treating bone fractures is the effective treatment of non-unions and delayed unions. Low Intensity Pulsed Ultrasound (LIPUS) has been approved by the FDA to treat fresh fractures since 1994 and non-unions since 2000 and is an attractive treatment option because it is …
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Studies on the Role of Vitamin D in the Mineralization of Bone: A Vitamin D-dependent Serum Protein That Stimulates the Incorporation of 45 Calcium by Bone Cells in Vitro
… in the 1920's established that rickets, a bone disease in which newly synthesized osteoid matrix does not calcify, was caused by a dietary deficiency in the fat-soluble vitamin D. These investigators concluded that vitamin D was necessary for the normal mineralization of bone. After 50 …
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The Regulatory Mechanism of Dietary Pattern in TMJ Condyle Growth
… of chondrocyte transdifferentiation into bone cells during postnatal condylar growth is unknown. Purpose: To quantify the effects of dietary loading on condylar chondrocyte transdifferentiation. Research Design: Chondrocyte tracing was performed in 21 Aggrecan-Cre;R26Tomato;2.3Col1a1-GFP …
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Testing the reliability and selectivity of different bone-cell-specific Cre- expressing mouse models for studying bone cell metabolism
… study focuses on the reliability of different bone cell specific Cre models in the Cre/loxP system. In this study, DMP1-Cre transgenic mouse which has a transgene driven by DMP1 promotor that allows Cre-expression only in late stage osteoblasts and osteocytes was used. Ctsk-Cre mouse with a …
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The Effects of Exposure to Extremely Low-Amplitude, Low-Frequency Electric Fields on Skeletal Tissue, In Vitro
… potentials are induced in impact-loaded bone, and (b) that bone formation is increased in response to electrical stimulation. Therefore, it has been hypothesized that, in response to mechanical loading, mechanical energy in bone is transduced into biologically relevant electrical energy. …
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Signaling mechanisms that suppress the anabolic response of osteoblasts and osteocytes to fluid shear stress
Bone is a dynamic organ that responds to its external environment. Cell signaling cascades are initiated within bone cells when changes in mechanical loading occur. To describe these molecular signaling networks that sense a mechanical signal and convert it into a transcriptional response, we …
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Deformation mapping of fibronectin fibrils using digital image correlation
Bone cells produce large amounts of extracellular matrix (ECM) proteins that form a fibrillar scaffold on which bone mineral is deposited. Prior work has shown that fibronectin fibrils are one of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of …
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Nmp4 restricts bone marrow osteoprogenitors and parathyroid hormone induced bone formation in healthy and estrogen depleted female mice
… gene. Additionally, these mice have increased bone marrow osteoprogenitors and CD8+ T-cells which support osteoblast differentiation. The animals were not protected from bone loss following OVX, but retained the hypersensitivity seen in the intact mice. Mesenchymal stem/progenitor cells …
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Kalirin : novel role in osteocyte function
Communication between bone cells is important for the maintenance of bone mass. Although osteocytes are deeply embedded within the mineralized matrix, they are essential for the regulation of osteoblast and osteoclast functions. However, the intracellular proteins that control the morphology and …
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In Vitro Regulation of Ptgs2 and Nos3 Expression in Clonal Osteogenic Cells Following Pulsatile Fluid Flow
… is a major health problem characterized by low bone mass and microarchitectural deterioration leading to enhanced bone fragility and increased fracture risk. In contrast, the high bone mass trait is characterized by increased bone mass and strength, with a significantly decreased fracture risk. …
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Cell engineering of human bone monolayers and the effect of growth factors and microcontact printed ECM proteins on wound healing. The role of ECM proteins, TGF¿-1, 2 and 3 and HCl/BSA in cellular adhesion, wound healing and imaging of the cell surface interface with the widefield surface plasmon microscope.
Bone repair is modulated by different stimuli. There is evidence that the Transforming Growth Factor-beta (TGF-¿) super-family of cytokines have significant effects on bone structure by regulating the replication and differentiation of chondrocytes, osteoblasts and osteoclasts. There is also …
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Polypyrrole : an interactive substrate for bone regeneration
Current methods of bone repair rely on autografts (bone from a donor site) and allografts (bone from human cadaver). However, these methods are plagued with disadvantages. There is a clear and urgent need to provide alternatives for regenerating and repairing bone. Bone is known to be one of the …
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