Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Blunt End"”.
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The Effect of Trypan Blue on the Growth Rate of the Chick Embryo During Development
… were made into the yolk sac through the blunt end of the egg during the 48th hour of incubation. Six independent variables, wet weight, dry weight, ash weight, organic content, weight of water and per cent of water of wet weight, were used to determine the effect of the dye on the embryos …
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Molecular cloning and analysis of the genome of bovine parvovirus
… of bovine parvovirus (BPV) has been cloned by blunt end ligation of double-stranded virion DNA into the plasmid pUC8. The resulting genomic clones were infectious after transfection into bovine fetal lung (BFL) cells. Sequencing of the plasmids demonstrated that deletions were common at both …
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… DNA (ss-DNA), primer-template DNA (pt-DNA), and blunt-end double-stranded DNA (ds-DNA) show that the binding selectivity pattern is similar when examined across a wide range of salt concentration, but can differ significantly at any individual salt concentration. For both proteins, binding of …
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Structure and Function of KH Domain in DDX43 and DDX53 Cancer Antigen Helicases
… ssDNA and ssRNA substrates efficiently but not blunt-end double-stranded (ds) DNA or dsRNA substrates. We applied 1H-15N HSQC NMR spectroscopy to determine the binding affinity of DDX43-KH with dT5, dA5, dC5, dG5 and rU5 oligonucleotides, and found that there are significant chemical shift …
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Single molecule studies of ligand-DNA interactions using atomic force microscopy
… termini of separate DNA strands in a sequence dependent fashion. This finding may have implications for the employment of these ligands in the nanosciences, as a tool for joining short pieces of DNA or improving the efficiency of the enzymatic, blunt-end ligation of DNA.
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Molecular Studies on Soybean Mosaic Virus-Soybean Interations
… prior RNA extraction. Amplified products were blunt-end ligated into pNoTA/T7 vector and transformed into competent cells. Sequencing was performed in both directions on heat-denatured double-stranded plasmids. The predicted 265 amino acid sequence of the CP of G1 and G6 strains were 98.9% …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
… begins when an enzyme complex binds to a blunt end of a dsDNA break. The complex unzips the dsDNA through its helicase activity, and simultaneously cleaves the newly-generated 5' end of the ssDNA. This process leaves the remaining ssDNA strand exposed to the surrounding environment and …