Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Blastomeres"”.
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ESTABLISHMENT IN CULTURE OF MOUSE AND HUMAN STEM CELLS WITH EXPANDED FATE POTENTIAL
The zygote and blastomeres of cleavage stage mouse embryos have the capacity to differentiate to the embryonic and both extra-embryonic lineages and are considered functionally totipotent. Until now, it has not been possible to establish stable cell lines that resemble these totipotent-like cells. …
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Cell Fate Decisions in the Early Mammalian Embryo
… factors, is a common feature. However, early blastomeres of mouse embryos have been reported to have distinct developmental fates, potential and heterogeneous abundance of certain transcripts, prior to the first cell fate decision. Nevertheless, the extent of the earliest intra-embryo …
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Fluorescence in Situ Hybridization Analysis of Human Embryos Derived From in Vitro and in Vivo Matured Oocytes
… no difference in the incidence of multinucleated blastomeres in the two groups, embryos derived from Prophase I oocytes had a significantly higher incidence of both anuclear blastomeres and blastomeres with a fragmented nucleus compared to their counterparts derived from mature oocytes. Because …
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Developing and Optimizing Conditions for Single Cell Genetic Analysis
… yielded 54% amplification rates using blastomeres that previously failed amplification. In the clinical trial, four of six blastomeres were diagnosed that had previous amplification failure. PEP and subsequent PCR testing is a useful means to diagnose samples that previously failed …
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C. Elegans OMA-1 and OMA-2 are Transcriptional and Translational Repressors of Germline Fate
… in the 4-cell stage embryo but only in somatic blastomeres. Global transcription is kept repressed by PIE-1 in germline precursors beginning with 4-cell stage to protect germ lineage from inappropriate somatic differentiation pathways. During my graduate studies, I investigated the redundant …
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Investigating the consequences of chromosome abnormalities arising during pre-implantation development of the mouse
… errors were induced in cleavage stage mouse blastomeres by bypassing the spindle assembly checkpoint (SAC). This model was used to investigate the fate of abnormal cells within the developing pre-implantation embryo, and the ultimate developmental outcome of mosaic embryos. Time-lapse imaging …
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Effect of different culture media and incubation methods on culturing murine embryos in vitro using a semen straw as an alternative receptacle
… were stained using Hoechst 33528 solution and blastomeres count. The results were analyzed by 2 X 3 factorial design and the student t-test was used to separate the mean and they showed that there was no statistica! difference (p > 0.05) between the media, receptacles and incubators. The …
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Post-Transcriptional Regulation of Maternal mRNA Shapes Early C. Elegans Embryogenesis
… that zif-1 is only translated in somatic blastomeres, beginning at the four cell stage embryo. Interestingly a similar set of RNA binding proteins (nine total) regulate the spatio-temporal expression of mom-2 in a similar fashion despite the fact that mom-2 has a reciprocal expression …
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The Effects of Propofol, Sodium Pentobarbital, and Ketamine Hydrochloride on <i>In Vitro</i> Mouse Embryonic Development
… of all three compounds. By the 72h endpoint, blastomeres of delayed and arrested embryos began to degenerate, displaying lysis and/or fragmentation; embryos exposed to lower concentrations of each anesthetic demonstrated a significant ability to continue cleavage and differentiation towards …
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Telomerase Activity in Human Preimplantation Embryos
… of measuring telomerase activity within biopsied blastomeres to predict blastocyst development. Telomerase positive (DU145 and PMEF) and negative (Hs27 and Detroit 551) cell lines were used to evaluate the assay system followed by the use of discard and donated oocytes and embryos for the …
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Comparative and phylogenetic analysis of the early embryonic development in the phylum Nematoda
… in cell cycle rhythms, cleavage order of the blastomeres, spatial configuration of the early embryo (based on cell-cell contacts and division angles), establishment of bilateral symmetry and the process of gastrulation. However, this diversity in early embryonic development does not result in …
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Size regulation of double and half embryos in the mice musculus
… by the isolation or destruction of one of the blastomeres of a 2-cell embryo are also born of normal size. Little is known about the timing or the mechanism of this size regulation. In this study, the timing of size regulation in double and half embryos was clearly established by comparison of …
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The role of polarisation in the first cell fate decision of the mouse embryo
… biased towards symmetric divisions and TE fate. Blastomeres with low activity of the arginine methyltransferase CARM1 prior to polarisation are known to be biased towards TE, and inhibition of CARM1, or overexpression of its substrate BAF155, increases the frequency of early polarisation. Thus, …
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The Effects of Toluene on the Ontogenetic Development of the Fish, Oryzias latipes, the Japanese Medaka
… were: (1) disruptions of the cleaving blastomeres, (2) malformation of the heart and vitelline circulatory systems, (3) deformation of the eyes, (4) malformation of the tail musculature as indicated by a bending of the tail, and (5) a failure of some of the visceral organs to develop …
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Karyopherin alpha subtypes and porcine early stage embryo development
… stage of development, while only a subset of blastomeres in 8-cell stage embryos possess nuclear BRN2. Octamer-binding transcription factor 4 (OCT4) adopts a nuclear localization in all nuclei prior to the 2-cell stage of development, whereas OCT4 is undetectable in nuclei at the 4-cell stage. …
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Mechanisms behind the fate of early chromosomal and transcriptional heterogeneities in the mouse embryo
… the transcriptional heterogeneities between the blastomeres of the 4-cell stage embryo affect subsequent lineage segregation. A high incidence of aneuploidy in the early cleavage divisions is considered the principal cause for low human fecundity and developmental defects. However, there is a …
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Protein phosphorylation and cell diversification in the mouse early embryo.
… flattening and delays polarisation of 8-cell blastomeres. In each case, the relative labelling intensity of some of the phosphoproteins characteristic of untreated 8-cell embryos was altered. The behaviour of these phosphoproteins suggests that they may be important in the mechanism by which …