Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"Blastocysts"”.
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Self-Renewal Requirements of Human Embryonic Stem Cells and Their Engraftment Potential in Mouse Blastocysts
… the ability of hESCs to engraft into mouse blastocysts. In advance of these cell mixing experiments, we derived a new hESC line, RUES1, and characterized its marker expression, functional characteristics and gene expression profiles. Using this new line, we showed that hESCs engrafted into …
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In-vitro developmental potential of bovine oocytes obtained by transvaginal follicular aspiration as related to their morphological quality and after microinjection of DNA
… A oocytes produced more viable embryos (31.7% blastocysts) than poor quality oocytes or SH oocytes (12.8% and 20.4% blastocysts, respectively). Embryo development following injection of DNA was the same for oocytes for each source-quality group (TVF A-good, 8.4; TVF A-poor, 5.5; SH-good, 6.3 % …
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Determining the Effects of Nerve Growth Factor Supplemented In-vitro Fertilization Media on Bovine Embryo Development
… rates and the number of hatching/ hatched blastocysts out of total presumptive zygotes. We hypothesize that NGF will improve IVP embryo development by positively influencing cleavage and blastocyst rates. The first two experiments' objectives were to determine the effect of recombinant …
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Investigating the Actions of Interleukin-6 Family Members on Post-Hatching Blastocyst Development in Cattle
… increased hatching rate of frozen and thawed blastocysts, but no cryotolerance effects were observed in thawed blastocyst cell number or percentage of apoptotic cells within the blastocyst. A separate series of studies tested if supplementing IL6 and/or LIF altered the morphology of …
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Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… stage, but slowed down afterwards, resulting in blastocysts with significantly lower cell counts than the 6% O2 group. The C57 embryos were slower to reach morula and expanded blastocyst, and had lower blastocyst rates in 2%O2 vs 6%O2. The C57 strain had significant slower overall embryo …
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ULTRARAPID FREEZING OF MOUSE EMBRYOS
… Upon differential staining of the developing blastocysts, it was followed that blastocysts derived from the phosphate-buffered T6 group possessed a significantly smaller inner cell mass (mean cell number of 21. 6) and a reduced mean total cell number (86.6) compared to blastocysts of tile …
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DNA methylation in the early porcine embryo
… the in vivo derived blastocyst does not occur in blastocysts produced by using in vitro techniques such as parthenogenesis, NT, and in vitro fertilization. Specifically, the methylation events that occur in the development of parthenogenetic and nuclear transfer blastocysts are more similar to the …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… cells, and most IL6-responsive cells in day 9 blastocysts were hypoblast cells (as measured by STAT3 activation). However, some epiblast (NANOG+) cells were also IL6-responsive, and IL6 appeared to initially slow epiblast differentiation. Finally, IL6-treated blastocysts also had increased …
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Characterization of Maternal Factor MOF/H4K16ac and the Impact of the Cryostress on Bovine Embryonic Development
… mouse and pig. Meanwhile, the exposure of bovine blastocysts was reported to induce severe cellular activities disorders, leading to inferior quality of cryopreserved offspring. In this thesis, we present 1) the characterization of the maternal factor MOF and H4K16ac dynamics in bovine embryo …
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Improving the development of bovine in vitro produced embryos cultured individually
… was found in the hatching rate between blastocysts cultured in media including cells (71.4 +/- 17.07%) and those cultured without cells (18.1 +/- 11.63%). In the second experiment, embryos were either cultured in standard droplets or microwells made at the bottom of culture droplets …
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The type I IFN of Bos taurus
… main aims 1) determine whether male and female blastocysts differ in the kind and number of IFNT they express and whether this pattern changes over development and 2) provide the first comprehensive annotation of the Type I IFN locus in Bos taurus, thereby providing an insight into the …
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Additive effects among uterine paracrine factors in promoting bovine trophoblast cell proliferation
… embryo development. In vitro produced bovine blastocysts were cultured either with the combination of growth factors or vehicle only from day 8 to day 12 post-in vitro fertilization (IVF). The combination of EGF, FGF2, and IGF1 increased (P<0.05) the percentage of hatched blastocysts and …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… remained hypomethylated in G₀- vs G₁-derived NT blastocysts, both in the inner cell mass and trophectoderm. Furthermore, G₀ donors significantly improved development into cloned blastocysts. In conclusion, quiescence induced long-term epigenetic changes, specifically H3K9me3 hypomethylation, that …
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Maintaining Proper Levels of DNA Methylation Marks Through the TET Family is Critical for Normal Embryo Development in Pigs
… increased expression of NANOG and ESRRB genes in blastocysts, although the DNA methylation levels of NANOG promoter were not changed. Interestingly, transcript abundance of TET3 was increased in blastocysts carrying inactivated TET1, which might have had an effect on the increase of NANOG and …
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Telomerase Activity in Human Preimplantation Embryos
… cell, 6–7 cell, 8–16 cell embryos, morulae, and blastocysts were evaluated individually for telomerase activity. Thawed zygotes cultured to day three were biopsied, by removing 1–2 cells and the biopsied embryos followed through culture to blastocyst. Telomerase activity was detected in positive …
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Lipid Quantification and Cryopreservation of In Vitro Produced Jersey Cattle Embryos
… + forsk/L-C). In vivo produced embryos (n = 27 blastocysts) were collected from superstimulated donors by routine procedures 7.5 days after AI. IVP embryos (n = 259 blastocysts) were produced by standard procedures; briefly, oocytes were aspirated from 2- to 8-mm follicles from slaughterhouse …
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Comparison of two different media and assisted hatching techniques on the embryo hatching rate using the mouse as a model
… chorionic gonadotropin (hCG). A total of 400 blastocysts were collected from the F1 generation and these were allocated equally for the four assisted hatching techniques (laser, mechanical, chemical and enzymatic) as well as a non-treated control group. The blastocysts were paired into a group …
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In vivo analysis of homing pattern and differentiation potential of cells deriving from embryonic and adult haematopoietic regions
… established cell lines injected into murine blastocysts find their way back home and seed preferentially at the site of their origin. Furthermore, can they change their fate and differentiate to unrelated cell types when exposed to the embryonic environment. This survey was based on the fact …
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Development of mouse morulae after encapsulation in alginate microgels or poly-l-lysine microcapsule
… of ZPI embryos (n = 150) from morulae to hatched blastocysts was measured after encapsulation in alginate or PLL and as unencapsulated controls. Following encapsulation, developmental stages were recorded every 24 h for 120 h. Percentage of encapsulated embryos completely hatched from the zona …
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