Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Blastocyst rate"”.
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DNA methylation in the early porcine embryo
… of the donor cells to direct development to the blastocyst stage was analyzed. The CpG methylation remodeling that occurs in the development of the in vivo derived blastocyst does not occur in blastocysts produced by using in vitro techniques such as parthenogenesis, NT, and in vitro …
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Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… incubators. Single-step media resulted in better blastocyst development compared to sequential media and the EmbryoScope™ incubation system showed slight improvements in embryo development than the Planer system. The benefits of supplementing the culture medium with either insulin or insulin-like …
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IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae)
… (Chapter 5) matured oocytes to develop to the blastocyst stage in culture was evaluated in subsequent studies. In Chapter 4, the hypothesis that the morphological characteristics of wood bison cumulus-oocyte complexes (COC) affect the ability of the immature oocyte to develop in vitro following …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
… and in each experiment, oocytes were aspirated from abattoir ovaries, matured for 23h, fertilized with semen from 1 of 3 bulls, and cultured in synthetic oviductal (SOF) based medium (SCF1) in 38.5 °C in 5% O<sub>2</sub>, 5% CO<sub>2</sub> and 90% N<sub>2</sub>. Stage 7 blastocysts were …
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IMPACT OF NUTRIENT IMBALANCE ON THE EPIGENETIC SIGNATURE AND GENE EXPRESSION OF BOVINE EARLY EMBRYOS
… focuses on how variations in energetic substrates during preimplantation development affect gene expression, differential transcript usage (DTU), and early developmental outcomes in bovine embryos. Using in vitro production techniques, bovine blastocysts were produced in standard and modified …
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Maturation and Culture Media Effects on In Vitro Bovine Embryo Developmental Competence
… N<sub>2</sub>. A proportion of stage 7 grade 1 blastocysts were fixed and stained with Nile Red to evaluate lipid content, Mitotracker Red CMX-Rosamine to measure mitochondrial activity, or Cell Rox Green to assess reactive oxygen species (ROS). Using a confocal microscope, images were taken of …
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Using CRISPR/Cas9 to modify the genome of cattle
… and pSpCas9(BB)-2A-GFP for gene transfer. The blastocyst rate was low and ranged from 0 to 20%. Although we did show that plasmid DNA was present on the sperm, we were not able to detect any positive GFP 8 day embryos using SMGT.