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Showing 1 to 20 of 22 for “"Black widow"”.
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Isolation and characterization of the tubuliform spidroin 1 promoter from the black widow spider, Latrodectus Hesperus
… and mapped the TuSp1 core promoter from the black widow spider, Latrodectus hesperus. Using a genomic DNA walking strategy, we have isolated an upstream segment (581 bp) of genomic DNA containing the promoter as well as the first exon of the TuSp1 gene. This upstream regulatory element was …
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Structural and mechanical analysis of the black widow spider web subjected to stretching, expansion and wind
The web of the black widow is apparently a random spatial arrangement of threads, which provides its occupant with housing, means to capture prey and protection. However to ensure that these functions are fulfilled, the spider has evolved to adapt the architecture of the web so that it would be …
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Structural characterization of spider coating petide [i.e., peptide] 1 and 2 of the black widow spider, Latrodectus hesperus
… coating peptides found on the silk fiber of the black widow spider, spider coating peptide 1 and 2, were investigated. Using circular dichroism, it was determined that SCP-1 and SCP-2 display predominantly alpha-helical secondary structures. In temperature gradient studies, SCP-1 is structurally …
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Evidence that aciniform silk and minor ampullate silk are major constituents of wrapping silk from the black widow
<p>Spider silk is one of the most remarkable materials produced in the natural world and its strength and extensibility are legendary. To date, the majority of studies have been performed on the following silks: dragline, capture spiral, and eggcase silk. The primary goal of this study was to …
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Molecular identification of silk proteins in the gumfooted lines and attachment discs of the black widow spider, latrodectus hesperus
… possibilities. The difficulties of having a black widow spider farm to harvest silk, due to their cannibalistic nature, make recombinant expression of silk proteins a fundamental goal of spider silk research. In order to express silk fibers, cDNAs encoding the corresponding silk fiber …
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The Effects of Black Widow Spider Venom and Cytochalasin-B on The Release of Acetylcholine From Superior Cervical Ganglia of Rats
Made available in DSpace on 2014-12-10T23:24:32Z (GMT). No. of bitstreams: 1 7405670.pdf: 5928878 bytes, checksum: f646f804f75274fcfea7762e0b2aa560 (MD5) Previous issue date: 1973
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Expression, purification, and characterization of a novel cysteine-rich silk protein expressed in the tubuliform and aggregate glands of the black widow spider : a thesis
<p>Belonging to the diverse order Araneae, the black widow spider Latrodectus 4 hesperus produces high-performance silks with a broad range ofbiological functions and mechanical properties. The cob weaver spider spins different fibers by using seven specialized glands located in its abdomen. Egg …
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Part I characterization of MyoR in C2C12 mouse fibroblasts. Part II isolation and characterization of a novel class II bHLH transcription factor from the black widow spider, latrodectus hesperus
… prepared from silk-producing glands of the black widow spider, we have identified a new bHLH transcription factor named BW6. Within the bHLH region, BW6 shows considerable conservation with other HLH proteins, including Drosophila melanogaster achaete and scute, as well as three HLH proteins …
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Generation of cDNA chips from the black widow spider, latrodectus hesperus, for gene discovery and expression profiling using microarray technology, and molecular characterization of a novel silk glue protein
… unknown genes from various silk glands of the black widow spider to profile their expression patterns and to identify novel candidates. Spiders are remarkable creatures because of their ability to make different silks, each with a specific function. Some of these silks have amazing mechanical …
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Generation of A L. Hesperus embryonic cDNA library for the isolation of genes involved in early pattern formation
… body pattern. To enable studies of the Westem black widow spider, Latrodectus hesperus, an embryonic eDNA library and a fixation protocol were developed for L. hesperus embryos. The generation of these tools will allow comprehensive analysis of black widow spider development and give insight …
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Characterization of the large diameter fibers in egg case silk : identification of a core fibroin, TuSp1, and localization of fibroin-like molecules, ECPs, from the black widow spider, latrodectus hesperus
… gene, named tubuliform spidroin 1 , from the black widow spider Latrodectus hesperus. TuSp 1 was demonstrated to be selectively expressed in the tubuliform gland (the gland suspected for egg case silk production), and examination of the amino acid sequence revealed highly homogeneous repeats …
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Characterization of a novel aggregate-gland-derived spider silk protein in Latrodectus Hesperus : from sequence to propsoed function
<p>Spider silk from the female black widow spider, Latrodectus hesperus, is made of extraordinary biomolecules of nature. Efforts of the scientific community to commercially synthesize silks have become a collaborating, yet competitive race, to characterize the proteins that contribute to its …
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Characterication of aggregate gland silk factor 1
… gland specific factor 1 (AgSF1) from the black widow spider, Latrodectus hesperus. After the development of an anti-AgSF1 polyclonal antiserum, we demonstrate by western blot analyses that the AgSF1 protein is highly expressed in the aggregate gland and the protein is localized to the …
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Characterizing small molecular weight proteins from Latrodectus hesperus dragline and tubuliform silks
… within dragline and tubuliform silks. Within the black widow spider, Lactrodectus hesperus, these proteins have been named Cysteine-Rich Protein (CRP) and determined to be a family of five individual proteins. The small protein identified within the tubuliform silks has been named Egg Case Protein …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
… were derived from the natural cDNA sequences of black widow spiders, while the internal block repeats were generated from synthetic DNA fragments that were codon-optimized for expression in Escherichia coli . Different numbers of internal block repeats were created using a specialized seamless …
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Investigation of Craniofacial Morphological Variation at Sully (39SL4)
… Rygh (39CA4), Mobridge (39WW1), Larson (39WW2), Black Widow Ridge (39ST203), Leavitt (39ST215), Cheyenne River (39ST1), and Indian Creek (39ST15) are examined to assess the factors contributing towards the phenotypic variation at Sully. The coordinate data were translated, rotated, and scaled via …
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Investigation of Craniofacial Morphological Variation at Sully (39SL4)
… Rygh (39CA4), Mobridge (39WW1), Larson (39WW2), Black Widow Ridge (39ST203), Leavitt (39ST215), Cheyenne River (39ST1), and Indian Creek (39ST15) are examined to assess the factors contributing towards the phenotypic variation at Sully. The coordinate data were translated, rotated, and scaled via …
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Synaptic vesicle recycling <i>in Vivo</i>
… durch das Gift der Schwarzen Witwe (Black Widow Spider Venom; BWSV) zur Diffusion dieser Proteine aus der Synapse in das Axon. In weiteren Experimenten konnten wir mithilfe des Kalzium-Ionophors Ionomycin zeigen, dass der molekulare Puffer vermutlich durch Kalzium kontrolliert wird …
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Identification of an aqueous glue protein, SCP-2, and the development of a polyclonal antiserum against the bHLH transcription factor SGSF in Latrodectus Hesperus
<p>Although numerous spider fibroins have been reported, no known silk coating peptides have been discovered. We provide the first biochemical evidence for a spider coating peptide, called SCP-2, found on gumfooted lines, scaffolding joints and egg cases. The presence of this spider coating peptide …
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