Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"BirA"”.
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Transcriptional regulation and enzymatic functions of the Escherichia coli biotin protein ligase
… directly regulated by the biotin-protein ligase, BirA, the enzyme that covalently attaches biotin to its cognate acceptor proteins. Binding of BirA to the bio operator requires dimerization of the protein, which is triggered by BirA-catalyzed synthesis of biotinoyl- adenylate (biotinoyl-5'-AMP), …
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Regulatory function and interdomain communication of group II biotin protein ligases
… biosynthesis. Escherichia coli BPL, called BirA, is the best-studied example of how an enzyme can also act as a transcriptional regulator of the biotin biosynthetic operon. Transcriptional repression of the E. coli biotin operon occurs when biotin acceptor proteins (biotin dependent enzymes) …
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PLASTICITY IN PROTEIN SEQUENCE-FUNCTION RELATIONSHIPS
… in the Escherichia coli biotin protein ligase (BirA) system, which serves as both an essential metabolic enzyme and a transcription regulator. In its repressor function, binding to the vitamin biotin allosterically activates BirA dimerization and the resulting repression complex assembly on the …
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Ritual ceremonial life: song text and spirit manifestation among the Zezuru People of Hwedza, Zimbabwe
… Shona subethnic group and they practise bira rematendo, (a ritual ceremony) in which songs are performed to pacify ancestral spirits. This thesis examines how song text fosters spirit manifestation in a Zezuru bira rematendo ceremonial performance. I further report on the origin of songs …
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Site-specific labeling of cellular proteins with unnatural substrates of biotin ligases
E. coli biotin ligase (BirA) catalyzes the site-specific ligation of biotin to the lysine within its 15-amino acid peptide substrate (AP). We harnessed the high peptide substrate specificity of BirA to develop a general methodology for site-specific protein labeling with biophysical probes. We …
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New reporters of protein trafficking and protein-protein interactions in live cells
… pair. We chose E. coli biotin ligase (BirA), which catalyzes the covalent ligation of biotin to a 15amino acid recognition sequence (AP). Only upon PPI would BirA be in close enough proximity to biotinylate the AP. Although the use of proximity biotinylation within the IF scheme proved …
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Molecular engineering of new protein labeling methodology based on rational design and in vitro evolution
… labeling using E coli biotin ligase (BirA) and its 15-amino acid "acceptor peptide" (AP) has been applied to study the function of various cellular proteins. In order to extend the capabilities of biotin ligase-based labeling, we engineered key elements of the labeling platform. First …
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Examining the role of water and hydrophobicity in folding, aggregation, and allostery
… such as the E. Coli biotin ligase/repressor (BirA), in which ligand binding promotes dimerization. We have used atomistic simulations of BirA mutants in collaboration with an experimental group to identify structural changes, accompanied by changes in solvation, at both the dimer interface and …
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Fatty Acid and Biotin Metabolism in Escherichia Coli
… levels of biotin in the growth medium via the BirA protein.
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Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
… enzyme turnover of the E. coli biotin ligase BirA, but found that our separation based on ultracentrifugation lacked the necessary sensitivity to discriminate between levels of enzyme turnover. We also looked at applications of the specific labeling of BirA and promiscuous labeling of …
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… by co-expressing it with a biotin protein ligase BirA in the follicle cells. As an extension of the application of the approach, I also show that the Torso-like protein, which is localized to the poles of the vitelline membrane and whose polar localization is crucial for Drosophila terminal …
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Enzyme-mediated labeling of proteins and protein-protein interactions in vitro and in living cells
The E. coli biotin ligase enzyme, BirA, has been previously used by the Ting research group for site-specific labeling of peptide-tagged cell surface proteins. We sought to expand the utility of biotin ligase-mediated labeling to functional group handles, including azides and alkynes, for …
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A Longitudinal Investigation of the Mental Health Benefits of Physical Activity Among Graduate Students
… of both researchers and non-researchers (Evans, Bira, Gastelum, Weiss, and Vanderford, 2018; Flatherty, 2018). This increased risk could be attributed to the stressors graduate students routinely experience. However, few studies have examined the negative effects of work stressors among graduate …
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Delineating the Mechanisms of Misfolded Endoplasmic Reticulum (ER) Luminal Protein Retrotranslocation for ER-Associated Degradation
… biotin-based reporter system. The bacterial BirA biotin ligase was tethered to FAM8A1, a component of the retrotranslocon that interacts with Hrd1, thus positioning the biotin ligase near the cytosolic exit site. The non-secreted immunoglobulin (Ig) κ light chain (NS1 κ LC), which is composed …
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Spontaneous and evoked vesicle cycling at a presynaptic terminal
… peptide (BAP). Co-transfection of biosyn with BirA, an enzyme that specifically recognizes the BAP sequence, results in the biotinylation of VAMP2. The subsequent addition of fluorescently-labeled streptavidin enables the visualization of newly exocytosed vesicles. I show in cultured …
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Enzyme-based reporters for mapping proteome and imaging proteins in living cells
… we utilized the Escherichia coli biotin ligase BirA and its acceptor peptide to site-specifically label the low-density lipoprotein receptor and studied its internalization and trafficking both at the ensemble imaging and single-molecule level. We discovered that this receptor internalizes as an …
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The Role of Psychological Flexibility in Graduate Student Stress and Well-Being
… health concerns among graduate students (Evans, Bira, Gastelum, Weiss, & Vanderford, 2018), research on graduate student mental health lags behind that of undergraduates (Hyun, Quinn, Madon, & Lustig, 2006). This study utilized Lent's (2004) social-cognitive model of well-being to examine factors …
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Forward and reverse genetic approaches to investigate cellulose biosynthesis in Physcomitrium patens
… higher incubation temperature, and large size BirA* tag, I was not able to identify any interacting proteins. Another finding of this thesis is that P. patens can be used to produce known natural products that are difficult to obtain by chemical synthesis. An in vivo combinatorial biosynthesis …
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Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… with the apoaequorin gene as well as the birA gene, encoding biotin protein ligase (BPL), responsible for post-translational biotinylation. The use of the biotin tag facilitated (a) the purification of aequorin from the crude cell extract using an avidin resin and (b) the direct …
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