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Showing 1 to 8 of 8 for “"Biotin Protein Ligase"”.
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Development of biotin protein ligase inhibitors from Staphylococcus aureus as new antibiotics
Biotin protein ligase (BPL) catalyses the ordered reaction of biotin and ATP to give biotinyl-5’-AMP 1.03, which then activates a number of biotin dependent enzymes that are critical to cell survival. Research undertaken in this thesis highlights strategies to selectively inhibit Staphylococcus …
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Transcriptional regulation and enzymatic functions of the Escherichia coli biotin protein ligase
Biotin metabolism in prokaryotes is directly linked to transcriptional regulation by the enzyme biotin protein ligase (BPL). This system is most thoroughly studied and characterized in Escherichia coli. Transcription of the E. coli biotin (bio) operon is directly regulated by the biotin-protein …
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… that transfers sulfate groups to several protein components of the vitelline membrane layer of the eggshell, including Vitelline Membrane-Like protein (VML). These sulfated proteins represent a ventral cue embedded in the eggshell, which, during embryogenesis, leads to the …
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PLASTICITY IN PROTEIN SEQUENCE-FUNCTION RELATIONSHIPS
… as the functional regulation at one site in a protein by activity at a distant site. Because of the ubiquitous occurrence and diverse cellular roles of allosteric proteins, designing novel allosteric proteins is of great interest for applications in synthetic biological and disease treatment. …
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Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… suitable for bacterial expression of the in vivo biotinylated aequorin photoprotein. The plasmid contained a biotin-acceptor coding sequence fused in-frame with the apoaequorin gene as well as the birA gene, encoding biotin protein ligase (BPL), responsible for post-translational biotinylation. …
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Regulatory function and interdomain communication of group II biotin protein ligases
Biotin is a cofactor required for function of essential biotin dependent enzymes that are involved in metabolic processes including fatty acid biosynthesis, gluconeogenesis, and amino acid catabolism. In order for biotin to act as a cofactor it must first be covalently attached to the biotin …
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Mechanisms of signal integration: Regulation of the SPI1 T3SS
… acting at the level of hilD translation or HilD protein activity. Once inside the phagosome of epithelial cells, or in macrophages during systemic stages of disease, the SPI1 T3SS is no longer required or expressed. Here we show that the PhoPQ two-component system, critical for intracellular …
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The synthesis, regulation and function of biotin in bacteria
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms