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Showing 1 to 20 of 295 for “"Biotin"”.
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Biotin synthesis in Escherichia coli
Biotin is an essential enzyme cofactor required by all three domains of life. It functions as a covalently-bound prosthetic group, which mediates the transport of CO2 in many vital metabolic carboxylation, decarboxylation and transcarboxylation reactions. Although biotin is essential, our knowledge …
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Biotin synthesis in α-proteobacteria
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-12-01
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Biotin synthesis in Bacillus subtilis
Biotin is a cofactor required by all three domains of life. It is an enzyme cofactor that acts as a “swinging arm” to transfer carboxyl groups in important metabolic pathways involving carboxylation, decarboxylation and transcarboxylation reactions. Despite its importance, the biotin biosynthetic …
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Control of Gluconeogenesis in Biotin-Deficient Rats
Made available in DSpace on 2015-05-12T22:36:27Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7219786.PDF: 5521611 bytes, checksum: d109163986b52797bd3a0f2da3be7e1b (MD5) Previous issue date: 1972
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Structural and Functional Studies of Biotin-Dependent Carboxylases
… remarkable precision. In humans, the family of biotin-dependent carboxylases holds important roles in intermediary metabolism. Recent years have witnessed significant progress toward understanding these enzymes' roles in homeostatic regulation. However, due to a lack of structural information, …
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The biosynthesis of a biotin compound containing ⁷⁵Se
… to determine if selenium could replace sulfur in biotin synthesized by Phycomyces blakesleeanus. Although selenium has been found to replace sulfur in many biologically active compounds, it has never been demonstrated in a naturally synthesized coenzyme or cyclic structure such as that found in …
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Fatty Acid and Biotin Metabolism in Escherichia Coli
Biotin which is essential for the synthesis of fatty acids in E. coli is thought to be derived from a modified fatty acid pathway. In experiments used to verify this hypothesis I discovered a new intermediate in biotin synthesis. This intermediate `X', was purified from the spent growth medium of …
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Carbohydrate Metabolism in Biotin-Deficient and Control Rats
Made available in DSpace on 2014-12-09T14:29:03Z (GMT). No. of bitstreams: 1 6910816.pdf: 3114363 bytes, checksum: 576d4f71f1217af055b51ff59178cf6c (MD5) Previous issue date: 1968
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Lipoate-protein and biotin-protein ligases of Escherichia coli
Lipoate and biotin are critical and almost universally distributed cofactors which share striking parallels. Both cofactors are covalently attached to specific lysine residues of specific apoprotein domains, and thus function as protein bound carriers of reaction intermediates between separate …
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The synthesis, regulation and function of biotin in bacteria
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms
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Studies on biotin as a coenzyme of propionyl carboxylase
A soluble enzyme system has been isolated from biotin-deficient rat liver acetone powder which catalyzes the synthesis of propionyl holocarboxylase from d-biotin and its apocarboxylase, in the presence of ATP and Mg++ ions. The enzyme system has been partially purified by (NH₄)₂SO₄ precipitation …
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The role of cysteine 230 and lysine 238 of biotin carboxylase in the deprotonation of biotin and synthesis of bisubstrate analogy inhibitor of carboxyltransferase
… coli form of the enzyme consists of a biotin carboxylase protein, a biotin carboxyl carrier protein, and a carboxyltransferase protein. This enzyme uses the cofactor biotin as a carboxyl carrier. In order for the carboxylation of biotin to occur, biotin must be deprotonated at its N-1 …
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Regulatory function and interdomain communication of group II biotin protein ligases
Biotin is a cofactor required for function of essential biotin dependent enzymes that are involved in metabolic processes including fatty acid biosynthesis, gluconeogenesis, and amino acid catabolism. In order for biotin to act as a cofactor it must first be covalently attached to the biotin …
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Development of biotin protein ligase inhibitors from Staphylococcus aureus as new antibiotics
Biotin protein ligase (BPL) catalyses the ordered reaction of biotin and ATP to give biotinyl-5’-AMP 1.03, which then activates a number of biotin dependent enzymes that are critical to cell survival. Research undertaken in this thesis highlights strategies to selectively inhibit Staphylococcus …
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Transcriptional regulation and enzymatic functions of the Escherichia coli biotin protein ligase
Biotin metabolism in prokaryotes is directly linked to transcriptional regulation by the enzyme biotin protein ligase (BPL). This system is most thoroughly studied and characterized in Escherichia coli. Transcription of the E. coli biotin (bio) operon is directly regulated by the biotin-protein …
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Studies of lipoic acid and biotin metabolism in bacteria and higher eukaryotes
… focus has been the study of lipoic acid and biotin metabolism in several bacteria. Lipoic acid and biotin are essential enzyme cofactors found in all three domains of life. Both molecules function only when covalently attached to key metabolic enzymes, 2-oxoacid dehygrogenase (OADH) and …
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The role of heterodera glycines biotin synthase in the nematode-soybean interactions
… HGTs. One of the candidate genes, H. glycines biotin synthase (HgBioB), contained sequence polymorphisms between avirulent and virulent inbred SCN strains. To test the function of avirulent and virulent HgBioB alleles, a complementation experiment using mutant Escherichia coli with these two …
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Site-specific labeling of cellular proteins with unnatural substrates of biotin ligases
E. coli biotin ligase (BirA) catalyzes the site-specific ligation of biotin to the lysine within its 15-amino acid peptide substrate (AP). We harnessed the high peptide substrate specificity of BirA to develop a general methodology for site-specific protein labeling with biophysical probes. We …
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