Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Biotherapeutics"”.
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In silico tools for the development of biotherapeutics
The production of a new drug is an inherently risky process. While there are many causes for failure, a number of these are due to the many potential degradation pathways a protein can undergo. Often these reactions are slow and negligible. However, there are times where any one of these reactions …
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Engineering targeted proteins for intracellular delivery of biotherapeutics
Biotherapeutics have revolutionized medicine with their ability to achieve unprecedented molecular recognition and mediate complex biological responses. The intracellular delivery of biotherapeutics is an unmet scientific challenge and medical need. A wide variety of different treatment modalities …
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Aggregation of IgG mAb Biotherapeutics: Sources, Methods of Characterization, and Biological Implications
… suggest that protein aggregates in certain biotherapeutics can decrease efficacy or cause untoward immune responses in human patients. Current research suggests that certain types of protein aggregates may be more immunogenic than others. In this Ph.D. thesis research work, three different …
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A platelet-mediated paradigm for the targeted delivery of microencapsulated, clot-augmenting biotherapeutics
… controlled “burst” release of an encapsulated biotherapeutics. As platelets are the “first responders” in the blood clot formation process, this platelet-hybridized system is ideal for the targeted delivery of clot augmenting biotherapeutics wherein immediate therapeutic efficacy is required. …
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Polymeric nanoparticle as a new platform for high removal of endotoxins from biopharmaceutical solutions
… to mammalian cells. On extracting the useful biotherapeutics from the gram-negative bacterial cells (<em>E.coli</em>), endotoxin present in the bacteria is released in the surrounding media thus contaminating the lifesaving biotherapeutics. Application of the endotoxin-contaminated …
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Subcellular Fractionation Methods for the Quantification of Endogenous Protein in Human Liver Using Targeted Proteomics
… models for immunoglobulin G- or albumin- based biotherapeutics. Second, UPLC-MRM methods were developed for a panel of organelle marker proteins to accelerate the quality assessment of organelles isolated from a sucrose gradient. Lastly, commercially available organelle isolation kits were …
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Design and screening of degenerate-codon-based protein ensembles with M13 bacteriophage
… development of high-value proteins, including biotherapeutics. While computational modelling increasingly facilitates protein design, empirically screening large collections of protein variants remains an essential component of protein engineering. This process requires generating protein …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… – in particular monoclonal antibody (mAb) biotherapeutics. Despite the availability of other platforms, CHO cells continue to dominate as the most utilised system due to their scalability, ability to perform post-translational modifications and long history of regulatory approval. Indeed, …
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UNDERSTANDING THE MOLECULAR BASIS OF CELL DEATH MEDIATED BY ONCOSIS-INDUCING CYTOTOXIC ANTIBODIES
… NOT CAUSE CELL DEATH. THESE MABS ARE POTENTIAL BIOTHERAPEUTICS AS THEY BIND A TUMOUR-SPECIFIC EPITOPE AND EXHIB
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Optimisation of CHO Cell Line Development Using Hybrid Modelling for Antibody Therapeutics
… particularly for personalised medicines and biotherapeutics, which hold the potential to be the safest and most effective therapeutic of all [1, 2]. Herein I propose a novel method for optimising upstream biopharmaceutical process scaling up using a data-driven hybrid model that integrates …
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Macromolecule Loading on PLGA Particles through Surface Adsorption
<p>The advent of biotherapeutics have impacted the ways in which diseases are treated. In many instances development of recombinant proteins and oligonucleotides into therapeutics are slowed by poor stability of the candidate drugs. Progress has been made in using polymeric particles as carriers of …
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User-centered automation process in synthetic biology research
… is advancing a wide range of domains from biotherapeutics for fatal cancers to biofuels and artificial meat to improve the global environment and food security. As the scale and complexity of synthetic biology endeavors are increasing, designing automation processes to replace manual labor …
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Identification and Characterization of MicroRNAs Modulating Cardiac Hypertrophy
… a potential lead for the development of new biotherapeutics.
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Transdermal delivery of heavy chain only antibody binding domains using hydrogel-forming microarray patches
… the transdermal delivery of ‘next generation’ biotherapeutics. This thesis also aimed to address the requests of patients from a previous study by designing a novel feedback mechanism to confirm complete MAP skin insertion. With further modification, this could help facilitate successful MAP …
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Rational engineering strategies to improve antibody fragment pharmacokinetics and bioavailability
… become one of the most widely used classes of biotherapeutics due to their unique antigen specificity and their ability to be engineered against diverse disease targets. There is significant interest in utilizing truncated antibody fragments, including single-domain antibodies (sdAb), as …
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Inhibiting Protein-Protein Interactions using Rationally-designed Repeat Proteins
… of this technology for the creation of future biotherapeutics.
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The Development of Vinylheteroarene Linkers for Proteinogenic Cysteine Modification and Studies Towards Applying (+)-Discodermolide as a Novel Payload in Antibody-Drug Conjugates
… makes them suitable for the generation of biotherapeutics or studies in chemical biology. The second project describes studies towards using (+)-discodermolide as a cytotoxic payload in ADCs. Discodermolide is a polyketide natural product which displays potent activity as an antimitotic …
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MEMS-based temperature-dependent characterization of biomolecular interactions
… in fundamental sciences and drug discovery and biotherapeutics. Micro-Electro-Mechanical Systems (MEMS) technology holds great potential in facilitating temperature-dependent characterization of biomolecular interactions by providing on-chip microfluidic handling with drastically reduced sample …
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The Development of Methods for Generating Fc-Conjugates and Antibody-Drug Conjugates
Biotherapeutics, such as peptides and proteins, constitute a valuable class of drugs due to their high target specificity and their ability to modulate protein interactions (PPIs), which are often considered ‘undruggable’ by traditional small molecule drugs. Additionally, antibodies possess many …
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Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
Gene therapies are a novel group of biotherapeutics based upon the delivery of nucleic acids to target cells. For delivery directly into a patient, termed in vivo gene therapy, recombinant adeno-associated virus (rAAV) vectors have taken centre stage. The most common method by which rAAV vectors …
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