Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 95 for “"Biosynthetic pathways"”.
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Interactions of the sterol and lipid biosynthetic pathways in yeast
Um neue Interaktionen zwischen dem Sterol- und anderen Lipidbiosynthesewegen zu identifizieren, wurde Saccharomyces cerevisiae genetisch verändert und der Gehalt der Fettsäuren und der Sterole wurde indiesen Stämmen untersucht. Die Überexpression von ACC1 im Wildtypstamm und im HMG-deregulierten …
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Studies of the purine biosynthetic pathways in the gastrointestinal tract.
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1979.
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The relevance of bacterial isoprenoid biosynthetic pathways for host-microbe interactions
… the relevance of two bacterial isoprenoid biosynthetic pathways (Mevalonate (MVAL) and 2-C-methyl-D-erythritol 4-phosphate (MEP)) for host-microbe interactions. We determined a significant reduction in microbial diversity in the murine gut microbiota (by next generation sequencing) …
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… wall. Several anti-TB drugs target cell wall biosynthetic pathways. A good understanding of its biosynthesis will provide helpful clues for the development of novel drug targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony …
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CRISPR-based Control of Biosynthetic Pathways and Genetic Biosensors, Informed by a Quantitative Investigation of Metabolic Burden
… heterologous, engineered genetics often used for biosynthetic chemical production share resources with host gene expression. While the host’s endogenous expression has evolved along with complex regulatory networks to balance supply and demand, or cost and benefit, heterologous expression requires …
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Targeting Trehalose and Methylglucose Lipopolysaccharide Biosynthetic Pathways in M. tuberculosis - Structural and Functional Characterisation, and Early-Stage Drug Discovery of OtsA and Rv3030
… M. tuberculosis possesses two pathways for synthesis of trehalose, the OtsA-OtsB pathway and the TreY-TreZ pathway. Multiple biosynthetic pathways underline the critical role that trehalose plays in the bacteria. OtsA-OtsB is the dominant pathway, required for bacterial growth …
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Molecular characterization of phosphonate biosynthesis in nature
… in nature is still not well understood. The biosynthetic pathways of almost all known phosphonates share the same initial step, in which phosphoenolpyruvate (PEP) is isomerized to phosphonopyruvate (PnPy) by PEP mutase (PepM). By using the pepM gene as a molecular marker for phosphonate …
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Biosynthesis and medicinal chemistry of therapeutically promising plant natural products
… the elucidation of plant specialized metabolite biosynthetic pathways. Identifying and characterizing the enzymes involved in specialized metabolite biosynthesis will provide insight into the evolution of enzymes performing interesting chemistries and provide new tools for the enzymatic …
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The Development of Di-N-Acetyl-L-Pyranosides for the Synthesis of Pseudaminic Acid and 8-epi-Legionaminic Acid
… the key intermediates in the biosynthetic pathways of Pseudaminic acid and 8-epiLegionaminic acid from L-fucose and L-rhamnose respectively. Chapter 1 gives a brief introduction to immunity and bacterial nonulosonic acids; particularly, Pseudaminic acid and 8-epi-Legionaminic …
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Genetic and Computational Tools for Engineering Complex Prokaryotic Systems
… significant challenges for engineering efficient biosynthetic pathways. While CRISPR-Cas technologies have revolutionized genome editing in eukaryotes, the development of programmable, multi-layer transcriptional control in bacteria is still emerging. This work outlines our efforts to expand the …
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Biosynthetic and Chemical Investigation of Lipid II-Binding Antimicrobials.
… understanding of structural components and biosynthetic pathways can lead to the creation of second-generation derivatives to improve bioactivity and pharmacological properties. To explore this superfamily, we have used bioanalytical, biochemical, synthetic, computational, and enzymatic …
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The Mitochondrial Fusion Protein, Optic Atrophy 1 (OPA1), Alters Cellular Metabolism to Support Cancer Cell Viability
… formation and triggered activation of stress pathways. Furthermore, profiling of OPA1-silenced cells revealed reprogramming of steady-state intracellular metabolites spanning intermediates of amino acids, TCA cycle and nucleotide biosynthetic pathways. To investigate nutrient usage, we …
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Mechanisms of Pteridine-Ommochrome Interactions in Drosophila Melanogaster
… . Therefore, an interaction between the two biosynthetic pathways is indicated.
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Secondary problems caused by the oxyr response to hydrogen peroxide stress in Escherichia coli
… the iron-dependent enzymes in different biosynthetic pathways. Therefore, OxyR induces the chaperones ClpS and ClpA, which are a part of the Clp family of proteases. Together with the ClpX chaperone and the ClpP protease, ClpSA helps increase the intracellular iron levels, enabling the …
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Production of pentanol in metabolically engineered Escherichia coli
… of my thesis work is to construct pentanol biosynthetic pathways in Escherichia coli, offering the possibility of producing pentanol from renewable carbon sources through microbial fermentations. We used butanol synthesis as a platform from which microbial synthesis of pentanol can be …
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Synthetic biology approaches for engineering diverse bacterial species
… or materials by initiating expression of biosynthetic pathways in response to specific signals. In this thesis, two new genetic tools were developed to aid the construction of genetic circuits and facilitate their delivery to bacteria isolated from diverse environments. First, antisense …
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Beach1 functionally antagonizes Rab11 during development and in regulating synaptic morphology
… plasma membrane via both the recycling and the biosynthetic pathways. Although beach1 loss-of-function mutants exhibit no obvious phenotypes, a sensitized background of a rabll mutant revealed a requirement for beach1 during development and in bristle extension.
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Enhanced yield of medicinal products from Tripterygium cell cultures
… of potential intermediates of the natural biosynthetic pathways, the first step in a technique where synthetic elaborations would be completed by cell cultures. These synthetic intermediates were also sought to establish the details of triptolide biosynthesis. Advances were made towards the …
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Fatty acid metabolism of marine microalgae
Pathways for the biosynthesis of docosahexaenoic acid (DHA) and other polyunsaturated fatty acids (PUFA) were elucidated in two heterotrophic, marine microalgae; Schizochytrium sp. and Crypthecodinium cohnii. PUFA-requiring auxotrophs of both of these algae were created and used as tools for …
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The design and synthesis of novel potential antimalarial compounds
… towards potential inhibitors for individual biosynthetic pathways.
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