Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 52 for “"Biosynthetic Gene Clusters"”.
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Genome Mining for Actinomycete Biosynthetic Gene Clusters
… (8-12 Mbp) genomes of Streptomyces and allied genera of Gram-positive filamentous bacteria house a rich and previously underestimated repertoire of gene clusters for biosynthesis of specialised metabolites, including antibiotics, immunosuppressants and anticancer compounds. Many of these …
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Identification and Characterization of Antibiotic Biosynthetic Gene Clusters in Pantoea
… search has been predominantly limited to a few genera. One underexplored bacterial genus is Pantoea, which produces a variety of compounds with antimicrobial activity. Chapter one describes a general overview of secondary metabolites, antibiotic resistance, strategies to overcome resistance, and …
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Investigations of peptide aminoacyl tRNA ligase (PEARL) biosynthetic gene clusters
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Novel Molecules from Environmental DNA-Derived Type II Polyketide Biosynthetic Gene Clusters
… a library was leveraged to take advantage of the genetic diversity of uncultured microbes. Uncultured microbes have been found to be rich in type II polyketide synthase (PKS) gene clusters (Kieser, Bibb et al. 2000). PKS gene clusters produce a structurally diverse collection of aromatic natural …
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Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms
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A path to biodiscovery: isolating Antarctic ascidian-associated bacteria and screening genomes for secondary metabolite-encoded biosynthetic gene clusters and signatures of cold adaptation
… results that suggest substantial natural product biosynthetic potential. To advance this research requires investigating cultivation strategies for the Verrucomicrobia-affiliated palmerolide A producer, establishing a more comprehensive understanding of the biosynthetic potential of other microbes …
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Natural Product Biosynthesis In Uncultured Bacteria
… majority. The heterologous expression of biosynthetic gene clusters cloned from DNA extracted directly from environmental samples (eDNA) has begun to provide access to the chemical diversity encoded in the genomes of previously uncultured bacteria. The systematic exploration of natural …
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Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha
… and adaptogenic properties. However, the biosynthetic pathway to withanolides is largely unknown, preventing scale-up and hindering pharmaceutical applications. In this thesis, we report a chromosome-scale assembly of the W. somnifera genome, which we use for biosynthetic gene cluster …
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Ecology and Synthetic Biology as Tools for Natural Products Discovery From Marine Proteobacteria
… in genomic sequencing, genomic analysis, and genetic engineering, it has become clear that only a small portion of the bioactive secondary metabolites that marine bacteria are capable of producing have been characterized. This thesis is an effort to aid in uncovering new chemistries and new …
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Studies of new natural products and their biosynthesis spearheaded by high-resolution mass spectrometry
… chain can be measured as a mass shift to the biosynthetic proteins by mass spectrometry. Specifically, Fourier-transform mass spectrometry (FTMS) (and an associated suite of custom MS tools) is a high-resolution analytical technique well suited to the study of NRPS and PKS enzymes because of …
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Comparative genomics of bacteria from amphibian skin associated with inhibition of an amphibian fungal pathogen Batrachochytrium dendrobatidis
… These 40 isolates spanned 11 families and 13 genera. The aim of this study was to identify genomic differences among the amphibian skin bacterial isolates and generate hypotheses about possible differences that could contribute to variation in their ability to inhibit the growth of Bd. We …
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Structure Determination of Natural Product Biosynthesis Enzymes
… challenging biologists to ascertain the genetic origin and catalytic logic affording intricate NP biosynthesis. Beyond the basic research inspired by multiplicity of NPs discovered thus far, the biological activities accompanying the unprecedented chemical architectures has generated …
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Investigation of cytochrome P450s in the biosynthesis of rice phytoalexins
… bioactive diterpenoids. Intriguingly, two biosynthetic gene clusters (BGCs), composed of CYPs and upstream cyclases dedicated to LRD production, have been discovered in the rice genome. The chromosome two BGC (c2BGC) contains a set of closely related CYP76M sub-family members that function …
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Oxygen-dependent enzymes involved in phosphonate biosynthesis
… we now have the ability to quickly identify new biosynthetic gene clusters with the capacity to produce new natural products and quickly identify the genes required for the production of a natural product. Characterization of these biosynthetic gene clusters allow us to identify the enzymes …
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An in silico approach to understanding the role of P450s involved in secondary metabolites production in mycobacterial species
… (presence of more than one copy of the same gene) and expansion (presence of the same gene in many species) were observed at the family and subfamily levels. CYP135 was the dominant family in mycobacterial species. The mycobacterial species have distinct P450 profiles, indicating lifestyle …
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Heavy metal inducible antimicrobial activity of streptomyces spp. isolated from the Leadhills and Wanlockhead lead mines in Scotland, Uk
… that Streptomyces spp. possess a range of biosynthetic gene clusters for secondary metabolites, however many of them are silent and cryptic under conventional laboratory conditions. It has also been shown that triggering the expression of these cryptic gene clusters in Streptomyces might …
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Synthetic biology approaches for engineering bacteria as living therapeutics
… takes this concept one step further through genetically introducing novel functions into bacteria to provoke a targeted therapeutic effect in humans. However, none of the engineered living therapeutics that have progressed into clinical trials have been approved for therapeutic use. I pursued …
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Rational Engineering of Expression Level of Multi-Gene Systems encoding Natural Product Biosynthesis in Streptomyces
… genus, harbor unprecedented numbers of biosynthetic gene clusters (BGCs) potentially encoding novel compounds. However, cloning and controlled expression of these large BGCs in a heterologous host require tedious optimization on a case-by-case basis. This dissertation presents a …
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Nucleoid-Associated Proteins of Streptomyces coelicolor: Discovery and Functions
The regulation of gene expression from biosynthetic gene clusters is a key concern in natural product discovery as these clusters are often transcriptionally silent (“cryptic”) under normal laboratory conditions, making the initial characterisation and heterologous expression of the products they …
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Characterization of microbial primary and secondary metabolism in the marine realm
… I applied genome mining tools to analyze the gene content and expression of biosynthetic gene clusters (BGCs). The analysis of BGCs through largescale genome mining efforts has identified diverse natural products with potential applications in medicine and biotechnology. Many marine …
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