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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Bioorthogonal chemistry"”.
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Developing unstrained alkenes and alkynes for bioorthogonal chemistry
Bioorthogonal reactions, due to its excellent selectivity and time-efficiency, have emerged as a popular tool for protein and cell probing. Among all the bioorthogonal reactions, the inverse electron-demand Diels–Alder reaction (IEDDA) reaction has its advantage of bearing the fastest kinetics. …
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Leveraging Bioorthogonal Chemistry for Hyperpolarized Magnetic Resonance and Metal Uncaging
<p>Bioorthogonal chemistry is a burgeoning area of study that has innumerable applications. This dissertation describes applications of bioorthogonal chemistry to solve problems in the fields of imaging and metal uncaging. We first consider hyperpolarized magnetic resonance, a powerful tool in …
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Discovery of prognostic biomarkers from the prostate cancer membrane proteome using bioorthogonal chemistry
… In summary, we have demonstrated the use of bioorthogonal chemistry for detecting differential sialyation and N-acetylgalactosamine glycosylation of proteins from human cell lines that represent the progression of cancer. Our data suggest that differential glycosylation may be a more …
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Interfacial bioorthogonal chemistry for biomaterials synthesis and patterning and development of catalytic method for "turning-on" the tetrazine ligation
The bioorthogonal trans-cyclooctene-tetrazine ligation has emerged into a powerful tool in the field of biomedical research. The development and the versatile applications of tetrazine ligation was made possible by the advancement of trans-cyclooctene synthesis. Based on the previous art of …
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Bioorthogonal Reagents: Design, Synthesis, and Reactivity
… that fit the stringent criteria for click chemistry and bioorthogonal chemistry has enabled discoveries and applications in fields ranging from materials science to chemical biology. These reactions must proceed rapidly and selectively under mild conditions. This enables the chemistry to be …
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Development of a Novel Bioconjugation Platform for the Generation of Homogenous Antibody-Drug Conjugates
… ratio (DAR) – all of which are controlled by the chemistry used to attach the drug to the antibody. Thus, to optimise these ADC characteristics, significant effort has been invested in the development of bioconjugation methods which yield homogenous ADCs with stable linkages and well-defined …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… has remained difficult for decades. Here, using bioorthogonal chemistry, we have developed a robust system to identify the proteins modified by lipids and advanced the ability to image cholesterol in cells and tissues. The unbiased proteomic analysis of fatty-acylated proteins using chemical …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
… low resolution or antibody-related biases. While bioorthogonal chemistry offers a powerful approach for RNA analysis, established reactions like the copper- catalysed azide-alkyne cycloaddition (CuAAC) can have disadvantages for modification mapping, including copper-mediated RNA degradation that …
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The Development of Divinyl-Heteroaryl Linkers for the Synthesis of Stable and Functional Antibody-Drug Conjugates
… linker or after rebridging through bioorthogonal chemistry. In addition, the receptor affinity and cellular selectivity of the antibodies were unaffected by rebridging and the antibody conjugates displayed exceptional stability in human plasma over two weeks. Critically, ADCs …
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On the aberrant nature of tau protein: biophysical approaches to characterise and target aggregation in neurodegeneration
… advantages of metadynamic structural biology and bioorthogonal chemistry to investigate the relative contributions of site-specific PTMs to the stability of tau-microtubule interactions. Our experiments, validated by *in silico* predictions, revealed a strong correlation between structural …
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Strain-promoted Stapled Peptides for Inhibiting Protein-protein Interactions
Protein-protein interactions (PPIs) are responsible for the regulation of a variety of important functions within living organisms. Compounds which can selectively modulate aberrant PPIs are novel therapeutic candidates for treating human diseases. Whilst PPIs have traditionally been considered as …
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Designed Bond Cleavage Reactions for Next-Generation Therapeutics
Creating ways for tissue-specific drug activation remains a significant challenge in modern medicine. Traditional approaches to drug delivery often lack precision, leading to unwanted side effects and reduced therapeutic efficacy. This PhD thesis explores two innovative approaches to overcome these …