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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"Bioorthogonal"”.
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Bioorthogonal Functionalization of Elastin-like Polypeptides
… functionality, we require the finesse of bioorthogonal chemistries and modification techniques.</p><p>Bioorthogonal reactions modify biological materials within living systems without perturbing function, much as two orthogonal lines can extend in different directions and intersect only at …
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Bioorthogonal Reagents: Design, Synthesis, and Reactivity
… the stringent criteria for click chemistry and bioorthogonal chemistry has enabled discoveries and applications in fields ranging from materials science to chemical biology. These reactions must proceed rapidly and selectively under mild conditions. This enables the chemistry to be performed in …
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Tetrazine-Triggered Bioorthogonal Decaging Reactions for Prodrug Activation
Bioorthogonal decaging reactions have emerged as a promising strategy for the spatially and temporally controlled activation of proteins and drugs. The inverse electron-demand Diels-Alder (IEDDA) reaction between tetrazines and strained alkenes exhibits high reaction rates and selectivity and has …
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Developing unstrained alkenes and alkynes for bioorthogonal chemistry
Bioorthogonal reactions, due to its excellent selectivity and time-efficiency, have emerged as a popular tool for protein and cell probing. Among all the bioorthogonal reactions, the inverse electron-demand Diels–Alder reaction (IEDDA) reaction has its advantage of bearing the fastest kinetics. …
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Leveraging Bioorthogonal Chemistry for Hyperpolarized Magnetic Resonance and Metal Uncaging
<p>Bioorthogonal chemistry is a burgeoning area of study that has innumerable applications. This dissertation describes applications of bioorthogonal chemistry to solve problems in the fields of imaging and metal uncaging. We first consider hyperpolarized magnetic resonance, a powerful tool in …
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Bioorthogonal Tethering of Drug Fragments to Engineered G Protein-Coupled Receptors
… employing genetic code expansion (GCE) and bioorthogonal chemical reactions for tethering drug fragments adjacent to allosteric sites in GPCRs to increase their potency and enable fragment-based drug screening in cellular systems. C-C chemokine receptor 5 (CCR5) was selected as a model …
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Multifunctionalization of Proteins: Strategies for Combining Semi-Synthetic and Bioorthogonal Techniques
Proteins play important roles in biological processes including muscle movement, initiating immune responses, and memory formation. Full understanding of protein function requires determining its structure, assignment of function, and elucidation of interactions with other proteins or metabolites. …
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Controlled metabolic cell labeling and bioorthogonal click chemistry for cancer targeting
… controlled metabolic cell labeling and bioorthogonal Click chemistry. By controlling the metabolic glycoengineering processes of unnatural sugars via rational chemistry designs, I was able to design a library of trigger-activatable sugar precursors that can label cell surface with …
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T-cell-directed bioorthogonal catalysis for localised prodrug activation in cancer therapy
… toxicities. This thesis investigates the use of bioorthogonal catalysis in combination with engineered T-cells to locally activate anticancer drugs. Catalysis-augmented T-cell therapy is a newly developed platform technology, in which T-cells, engineered to express a surface protein upon cancer …
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Development and application of bioorthogonal palladium- labile derivatives of cytotoxic pyrimidine analogues
… this thesis describes the pioneering use of a bioorthogonal organometallic (BOOM) activation strategy to develop spatially-controlled anticancer treatments. Bioorthogonal reactions are selective chemical processes between two abiotic reagents in a biological system that do not interfere with …
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Discovery of prognostic biomarkers from the prostate cancer membrane proteome using bioorthogonal chemistry
… In summary, we have demonstrated the use of bioorthogonal chemistry for detecting differential sialyation and N-acetylgalactosamine glycosylation of proteins from human cell lines that represent the progression of cancer. Our data suggest that differential glycosylation may be a more …
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A Pretargeted Spect Imaging Strategy Employing Technetium-99M Based on Bioorthogonal Diels-Alder Click Chemistry
… in pretargeting SPECT imaging that utilizes the bioorthogonal Diels-Alder click reaction between tetrazine (Tz) and transcyclooctene (TCO). The objective was to optimize the excretory pathways of the <sup>99m</sup>Tc-peptide complexes for maximum tumor targeting with the <em>in vivo </em>“click” …
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A multiplexed approach for quantitative profiling of the translatome using bioorthogonal non-canonical amino acids
One of the major goals of systems biology is understanding how a cell changes from a healthy state to a diseased state. Entire fields of cell biology have been built around studying how changes in the type and abundance of specific biomolecules affect disease status. However, one major knowledge …
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Bioorthogonal Reactions: Synthesis and Evaluation of Different Ligands in Copper Catalyzed Azide-Alkyne1,3-Dipolar Cycloaddition (CuAAC)
<p>The Copper CatalyzedAzide-Alkyne1,3-Dipolar Cycloaddition (CuAAC) reaction has unique features that qualify it to be one of the best click reactions. Its applications have been shown in different aspects and for multiple purposes. The oxidative degradation of biological systems (labile proteins …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… has remained difficult for decades. Here, using bioorthogonal chemistry, we have developed a robust system to identify the proteins modified by lipids and advanced the ability to image cholesterol in cells and tissues. The unbiased proteomic analysis of fatty-acylated proteins using chemical …
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Interfacial bioorthogonal chemistry for biomaterials synthesis and patterning and development of catalytic method for "turning-on" the tetrazine ligation
The bioorthogonal trans-cyclooctene-tetrazine ligation has emerged into a powerful tool in the field of biomedical research. The development and the versatile applications of tetrazine ligation was made possible by the advancement of trans-cyclooctene synthesis. Based on the previous art of …
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Hypervalent iodine reagents for protein functionalisation
… versatile conjugates that can undergo further bioorthogonal modifications. This powerful strategy proceeds with high efficiency and chemoselectivity across a range of small proteins and polypeptides. In this work, we describe the development of a new generation of methionine-selective protein …
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Methods for the structural modification and characterization of bacterial glycans
… Taking advantage of the development of rapid, bioorthogonal chemistries these probes have provided valuable insight into intermolecular interactions, biosynthetic and metabolic pathways, and intercellular interactions. The successful application of metabolic incorporation probes to bacteria has …
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