Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 86 for “"Bioluminescence"”.
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THE EVOLUTION OF BEETLE BIOLUMINESCENCE
… in this thesis examines the evolution of bioluminescence in beetles. The study utilises two enzymes implicated in the bioluminescent reaction of fireflies and glow-worms; the protein luciferase and the luciferin regenerating enzyme (LRE). A range of parallagous genes for both enzymes were …
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Vision and Bioluminescence in Cephalopods
… affects the ecology and evolution of vision and bioluminescence through an examination of cephalopods, a highly visual group that exhibits a broad diversity of eye adaptations and multiple evolutions of bioluminescence. My research investigates (1) vision and behavior in a deep-sea squid with …
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Bioluminescence Imaging Strategies for Tissue Engineering Applications
… and temporal manner prior to implantation. Bioluminescence imaging is a technique that has been utilized to monitor cell viability and gene expression in various in vivo applications. However, it has never been applied in an in vitro setting for the specific purpose of evaluating a …
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The evolution and specialized metabolism of beetle bioluminescence
… produce light in a process known as bioluminescence. The bioluminescent systems of beetles, natively used for the purposes of mating communication and/or an aposematic warning signal, are now well understood and have been widely applied in biotechnology and biomedical research. There …
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Real-Time Bioluminescence Imaging Of Salmonella-Neoplastic Cell Interactions
Salmonella Typhimurium is a Gram-negative bacterial pathogen and a common cause of gastroenteritis in humans. The organism utilizes a multitude of well-studied virulence factors to invade and replicate in host intestinal epithelial cells and macrophages. Interestingly, Salmonella is also capable of …
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Bioluminescence in dinoflagellates – diversity, molecular phylogeny and field ecology
… dinoflagellates are also capable of producing bioluminescence and they are the most abundant protists that produce light in the surface waters of the oceans. This<br/>study employed molecular tools to investigate the identity of bioluminescent species, the genetic basis, diversity and …
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Elucidating Mechanisms of Biofluorescence and Bioluminescence in Marine Organisms
<p>Biofluorescence and bioluminescence are two methods of light emission that entail separate mechanisms of action but end at the same process: a colorful display that have tremendous ecological and behavioral benefits, whether it be used to communicate with conspecifics, camouflage into a …
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Dynamic Characterization of the IKK:κBα:NFκB Negative Feedback Loop Using Real-Time Bioluminescence Imaging
… conclusion, our studies using dynamic, real-time bioluminescence imaging have demonstrated the utility of employing bioluminescent reporters alongside traditional biochemical assays, <em>in silico</em> modeling, and cell/molecular biology techniques to rigorously interrogate how diverse stimuli …
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Nad(p)h: Fmn Oxidoreductase of Photobacterium Fischeri: Its Role in Bacterial Bioluminescence
Made available in DSpace on 2014-12-09T17:36:33Z (GMT). No. of bitstreams: 1 7013292.pdf: 6207081 bytes, checksum: 4f051799ea2980f7923fe2b38e070660 (MD5) Previous issue date: 1969
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Estimation of microbial biomass in natural and perturbated tundra by the ATP bioluminescence assay
… soil population measurements and the ATP bioluminescence assay. Soils from various habitats typical of the north slope coastal landscape and oil, vehicle and temperature perturbations were examined. ATP content of the tundra soil was 4 g/gm soil in the 0 - 2 cm histic layer and 0.4 g/gm …
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The Relationship Between Soluble and Particulate Bioluminescence in Extracts of The Marine Dinoflagellate, Gonyaulax Polyedra
Made available in DSpace on 2014-12-10T23:00:59Z (GMT). No. of bitstreams: 1 7114745.pdf: 3238735 bytes, checksum: 97219d92ae123aa56c778ece6b9dfd17 (MD5) Previous issue date: 1970
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Convergent Evolution of Biochemical Innovations in Polychaetes: Characterizing the Molecular Basis of Bioluminescence and Venom Production
… molecular basis of two biochemical innovations, bioluminescence and venom production, that have evolved independently in different lineages of polychaetes worms (Annelida). We use an integrative approach, combining next-generation sequencing, phylogenetics and computational tools to evaluate …
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Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining
Bioluminescence imaging (BLI) is a powerful technique that enables real time monitoring of various processes such as gene regulation, host-pathogen interaction and distribution of bacterial cells in vivo. Most of these processes however have only been monitored in murine models or mammalian tissue …
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Bioluminescence-Based High Precision Bacterial Growth Rate Measurements and Their Potential Application in Quantifying Deleterious Effects of High Mutation Rates
… end, we constructed an apparatus that combines bioluminescence based growth rate measurements with techniques for long-term microbial culture for recording of growth rate dynamics of luminescent Escherichia coli. After a comparison of several culture media, we found that the reproducibility of …
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Development of Hif-1Α/Hif-1Β Heterodimerization Inhibitors Using A Novel Bioluminescence Reporter Assay System For In Vitro High Throughput Screening and In Vivo Imaging
… efficacy <em>in vivo</em> were verified by bioluminescence imaging (BLI) of HIF-1α/β heterodimerization in the xenograft tumor model. Furthermore, the first generation drug inhibitors, M-TMCP and D-TMCP, demonstrated efficacy as monotherapies, resulting in tumor growth inhibition via …
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I) Hv1, LBP and LCF reconstitution in Liposomes II) Signed, Sealed, and Delivered: CPP Technology Delivers Active Protein Cargo
<p>The first half of this thesis focuses on bioluminescence within the <em>Lingulodinium polyedrum </em>dinoflagellates. In this species, the organelle termed “scintillon” houses the components involved in bioluminescence: Luciferin Binding Protein (LBP), Luciferase (LCF), voltage-gated proton …
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Syntheses of luciferins and their bioluminescent evaluation
… an alternative or complementary substrate for bioluminescence imaging, that would allow for bioluminescence to be applied in systems where traditional D-luciferin application was limited. In the first part of the thesis, D-luciferin and a C6-amino analogue of D-luciferin, D-aminoluciferin, were …
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Responses of an Environmental Gram-negative Bacterium to Pollutant Stress
… and fresh water samples. To show the use of bioluminescence in the indigenous (naturally non-bioluminescent) organism, research was performed on stable marking and optimisation of light output including marking of the organisms with luxAB or the whole lux cassette, which necessitated …
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Variations in bacterial adenosine triphosphate values due to genus and environmental conditions
… and Pseudomonas sp. were investigated using the bioluminescence (luciferin-luciferase) assay. Environmental factors (temperature, atmosphere, pH, aeration, and phase of growth), as well as differences among genera and species, were studied in relation to their effect on cellular ATP. Variations …
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The HV1 proton channel of Lingulodinium polyedrum localizes to the bioluminescent scintillon
… with H<sub>V</sub>1’s proposed function in bioluminescence. Isolated scintillons immunostained with antibody to LpH<sub>V</sub>1 displayed LpH<sub>V</sub>1 expression, showing that LpH<sub>V</sub>1 is present in this organelle. In addition, proteomics analysis demonstrated that isolated …
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