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Showing 1 to 14 of 14 for “"Biokatalyse"”.
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Experimentelle Analyse, Modellierung und biochemische Charakterisierung von Ein- und Zweiphasenreaktionen für die technische Biokatalyse
Reaction engineering is a key aspect in the optimisation of chemical reactions. However biocatalysis faces a set of problems while implementing the interdisciplinary methods from biology, thermodynamics, and engineering science. Four issues are of mayor concern: (1) arbitrary simplifications in …
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Von cyclischen, olefinischen Kohlenwasserstoffen zu enantiomerenreinen, N,O-perfunktionalisierten C6/C12-Bausteinen
… (1) Stereoselektive N,O-Funktionalisierungen und Biokatalyse zur Gewinnung enantiomerenreiner Zwischenprodukte. (2)Synthese- varianten zur Transformation von cyclischen zu terminal-differenzierten, acyclischen C6-Bausteinen. (3) Pinakol-Homokupplung von C6-a-Aminoaldehyden zu C2-symmetrischen …
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Halogenasen aus Actinomyceten : funktionelle und phylogenetische Studien
… werden können. Neue Derivate wurden durch Biokatalyse, kombinatorische Biosynthese und die Variation von Fermentationsparametern hergestellt. Zusätzlich konnte durch die Nutzung der Transposon-Mutagenese erfolgreich eine Reihe neuer Xanthonderivate hergestellt werden.
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Biokatalytische enantioselektive Sulfoxidation
… Der Einsatz schwefelhaltiger Pestizide in der Biokatalyse verlief erfolglos, allerdings wurden die Organophosphorpestizide Fenamiphos® und Fenthion® mit dem aus Sulfoxidationsreaktionen lange bekannten Enzym Chlorperoxidase (CPO) enantiomerenangereichert umgesetzt. 4e. Die biotechnologisch …
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Expression, Reinigung und Charakterisierung von Makrolid-Glykosyltransferasen für die Synthese neuartiger Makrolid-Antibiotika
The macrolide glycosyltransferases EryCIII, EryBV, OleG2 and TylMII were produced by medium-scale fermentation of recombinant Saccharopolyspora erythraea strains. The soluble expression of all glycosyltransferase genes was verified by immunoblotting and ELISA against the C-terminal …
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Enantioselective enzymatic reduction of prochiral ketones in one-phase and two-phase systems
Within this thesis a strategy for the enantioselective synthesis of chiral short chain alcohols using biocatalysis is developed. This is realized in close collaboration with an industrial partner. The biocatalysts used are alcohol dehydrogenases (ADH) which need nicotinamide cofactors (NAD+/NADH …
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Biocatalytic carbon nitrogen double bond reduction
Within this research project the bioreduction of imines and oximes has been investigated as possible promiscuous activity of two different reductases families, the enoate reductase family (EC 1.3.1.31) and the carbonyl reductase family (EC: 1.1.1.1). The decision of targeting these enzyme classes …
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A computational approach to solvent selection for biphasic reaction systems
Biphasic reaction systems with a reactive and a non-reactive phase are widespread in technical applications. The non-reactive phase serves as a reservoir of dissolved substrates at high concentrations and allows for the extraction of the product during the reaction. The proper choice of the phase …
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Entwicklung eines Enzym-Modul-Systems zur Synthese und in situ Regeneration von dTDP-aktivierten Desoxyzuckern
The results of this thesis showed for the first time that it is possible to produce glycosylated natural compounds in vitro with in situ regeneration of dTDP-activated deoxysugars. The deoxysugar biosynthesis genes from the avilamycine- (aviS, aviT), urdamycine- (urdR) and midekamycine-biosynthesis …
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Elektroenzymatische Synthese mit ionischen Flüssigkeiten
The electrochemical generation, respectively regeneration of redox equivalents represents an interesting alternative to the for this purpose usually applied methods. However, this method has some drawbacks, which make their application as industrial processes economically unfeasible. Especially, …
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Thermodynamic and kinetic phenomena in the enzymatic conversion of acetophenone to 1-(R)-phenylethanol in a continuous gas/solid reactor
Nowadays, the Gas/Solid Biocatalytic Systems gain continuously ground within the area of the Non-Conventional Biocatalysis. In order to use the gas/solid catalysis for analytical and synthetical purposes, the respective process has to be understood in detail. Therefore, the construction and …
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Metagenombasierte Isolierung und biochemische Charakterisierung neuartiger stereospezifischer Lipasen für biokatalytische Anwendungen
Die Technik des Klonierens von DNA eines bestimmten Standortes, des. sog. Metagenoms, hat in den letzten Jahren erfolgreich dazu beigetragen, das genetische Potential aller dort vorhandenen Mikroorganismen zu erschließen, von denen ca. 95% als bis heute unkultivierbar gelten. Im Rahmen dieser …
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Charakterisierung und Optimierung gel-stabilisierter Zwei-Phasen-Systeme zur stereospezifischen Reduktion hydrophober Verbindungen mit der Carbonyl-Reduktase aus Candida parapsilosis
Enzymes are increasingly being used for the production of chiral compounds with high enantiomeric purity for the synthesis of fine chemicals and pharmaceuticals. Reason for this lies mainly in the high substrate and reaction specificity of many biocatalysts. Interesting starting compounds are often …