Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 140 for “"Bioinformatic analysis"”.
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MicroRNA cloning and bioinformatic analysis
… Therefore, we developed an experimental and bioinformatics strategy to identify novel miRNA genes. We discovered a total of 75 miRNA genes in the C. elegans genome, and orthologues for a majority of these were computationally identified in the C. briggsae, D. melanogaster or H. sapiens …
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Bioinformatic analysis: Retroposon sequences containing multiple putative endonucleases
Among the retroposons, the source of the endonuclease activity is known to be variable. We investigated whether any retroposon genomes encode potential multiple endonuclease functions. Amino acid alignments were generated to reveal the presence of the characterized ordered-series-of-motifs (OSM) …
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Bioinformatic Analysis of the Interaction Of EVC And EVC2 Proteins
<p>Protein to protein interactions are significant to various biological processes, functioning, and the regulation of cells and organisms. Proteins perform essential cellular tasks and often work together in complexes, enabling specific functions like enzymatic activity, signal transduction, DNA …
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Functional genomic study and bioinformatic analysis on natural bioactive peptides
… to the results from in vitro function alanalysis, this modification strategy significantly improved the selectivity index of thepeptide with increased antimicrobial activity and decreased haemolysis activity. The combined antimicrobial evaluation on both natural and modified peptides …
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Bioinformatic Analysis of Wastewater Metagenomes Reveals Microbial Ecological and Evolutionary Phenomena Underlying Associations of Antibiotic Resistance with Antibiotic Use
… treatment settings. Through application of novel bioinformatic approaches developed herein, metagenomics revealed associations between sewage-associated microbes and community antibiotic use that were in part mediated by microbial ecological processes and horizontal gene transfer (HGT). In sum, …
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BIOINFORMATIC ANALYSIS OF A MAMMALIAN BIP GENE FOR INSERTION INTO GREEN ALGAE AND COMPARISON OF ITS POSSIBLE EFFECTS ON THE SYNTHESIS OF A MAMMALIAN ANTIBODY
This dissertation describes a study utilizing bioinformatics to analyze homologues of a molecular chaperone, glucose-regulated protein 78 (grp 78), also known as BiP. The selected homologous proteins originate from organisms of infinitely diverse genera. Comparisons of protein sequence yielded the …
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Functional Characterization of Plasmodium falciparum TATA-box binding-like Protein (PfTLP)
… which is largely uncharacterized in Plasmodium. Bioinformatic analysis identified putatuve P. falciparum orthologues of RNA polymerase II general transcription factors (Bing, 2014; Milton, 2017), including a TATA box-binding-like protein, PfTLP. Bioinformatic analysis suggested that PfTLP is a …
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The Discovery and Evolutionary Analysis of Divergent Homologs Involved in Cattle Immune and Reproductive Biology
… homologs were identified using a subtractive bioinformatic analysis of 12,614 cattle placenta ESTs followed by comparative, evolutionary and gene expression studies. Eight have not been identified previously. These were named: CSSMST1, CIST1, HAVCRNDP, PRP8, PRP9, PRP11, SECTM1A and SECTM1B. …
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Assignment of Enzyme Function Through Characterization of the RuBisCO and Enolase Superfamilies
… that included structural, computational, and bioinformatic analysis as well as classical enzymology. The T. maritima and E. faecalis enzymes were subsequently confirmed as dipeptide epimerases with unique specificity for hydrophobic dipeptides through screening of dipeptide libraries by mass …
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Large-scale methods for multiple sequence alignment and phylogeny estimation
Bioinformatic analyses generally involve passing genetic sequence data through a pipeline of transformations. These prominently include multiple sequence alignment (MSA) and phylogeny estimation, which are both core challenges in computational biology. Their established mathematical formulations …
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Bioinformatics of High Throughput Proteomics Using Tandem Mass Spectrometry of Intact Proteins
… of proteins. Before this is possible, the bioinformatic analysis of top down mass spectrometric data needed to be automated. This work demonstrates a functional automated top down search environment called ProSightHT. Further, it derives and demonstrates the utility of an improved scoring …
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Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control
… The purification, expression, RNase testing and bioinformatic analysis of M. tuberculosis VapCs suggested that VapCRv2530c, VapCRv0065 and VapCRv0617 may all target the same recognition sequence, UA*GG. Bioinformatic analysis revealed an abundance of this target sequence in horizontal gene …
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Do RNA-dependent polymerases share common ancestry? A bioinformatic approach
Bioinformatics is the use of computational methods to perform hypothesis-driven research that generates new knowledge from existing biological databases. For any bioinformatic analysis, it is important that the most accurate method(s) be used. The first portion of this thesis is a comparative …
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Functional genomics in the stroke-prone spontaneously hypertensive rat: genome wide and candidate gene analysis
… mu type 1 (Gstm1). Ingenuity pathway analysis of renal microarrays in 5 and 16-week SHRSP, normotensive Wistar Kyoto (WKY) and chromosome 2 congenic rats identified differential expression of several glutathione cycling genes. The Gstm1 promoter was investigated by luciferase and …
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Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae
… isomerase activity was detected. An in silico bioinformatic analysis showed a putative inhibitory stem-loop structure in the mRNA containing the B. thetaiotaomicron gene which may have reduced translation. Otherwise non-functional folding or undetectable activity were concluded as the results …
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Genomics as a tool for natural product structure elucidation
… ease and affordability of large-scale genomic analysis. The ability to genome-gaze has not only revealed hundreds of thousands of yet-untapped secondary metabolites in sequenced organisms but also can facilitate strain prioritization, novelty determination (dereplication), structure …
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An analysis of selected secretion systems of Pseudomonas species
… clinical pathogens and biocontrol strains. Bioinformatic analysis of T6SS phylogeny and associated gene content within Pseudomonas identified several T6SS phylogenetic groups, and linked T6SS components VgrG and Hcp encoded outside of T6SS gene loci with their cognate T6SS phylogenetic …
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Apicomplexan Class XXVII Myosin - A Computational, Biophysical and Cell Biological Characterisation of PfMyoF in Plasmodium falciparum
… the essential P.falciparum myosin – PfMyoF. Bioinformatic analysis identifies novel features of both PfMyoF including two novel tail domains – a GTP binding and a WD40 domain. Bioinformatic, in vitro and cell-based assays have identified the regulatory light chains and suggest PfMyoF behaves …
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Molecular mediators of cardiac-specific enhancer activation
… toward cardiomyocytes (CMs). Based on bioinformatic analysis of these data, we identified the TEA domain family (TEAD) TF TEAD1 as a candidate regulator of enhancer activation during cardiac-lineage commitment. We then used an inducible degron-tag strategy to conditionally deplete TEAD1 …
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The role of copper proteins in the ammonia oxidation pathway of Nitrosopumilus maritimus
… as candidates for ammonia oxidation in AOA. Bioinformatic analysis of three of these proteins lead to the prediction that; Nmar1131 is a homotrimeric laccase-like 2dMCO, Nmar1354 a homotrimeric 3dMCO with an additional T1Cu site in the C-terminus cupredoxin domain, and Nmar1667 an NO-forming …
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