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Showing 1 to 20 of 23 for “"Biofilm matrix"”.
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Type IV Pili-Dependent Secretion of Biofilm Matrix Material Proteins in Clostridium perfringens
… is a Gram-positive bacterium that secretes a biofilm matrix material. The goal of these experiments was to identify pilin mutants that are needed for secretion of the biofilm matrix and develop a functional model for a type II secretion system (T2SS) in C. perfringens. Protein tagging, western …
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The structure and function of TasA in the <i>Bacillus subtilis</i> biofilm matrix
Biofilms are communities of microorganisms attached to a surface and encompassed within a self-produced extracellular matrix. The matrix functions to shield the interior cells from biotic and abiotic stresses and to retain nutrients and signalling molecules. The multiple functions of the biofilm …
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Exoproteomics of the Pseudomonas aeruginosa biofilm matrix reveals the role of a novel secretion system
… P. aeruginosa has to forming what are known as biofilms - heterogeneous aggregates of cells living in a matrix of their own secretions. Cells that would otherwise be susceptible to antimicrobials are protected by the modifications that they make, in concert, to their surroundings. Aggregates of …
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Heat resistance of Salmonella typhimurium and Listeria monocytogenes in suspension and in a biofilm matrix
… A suspended in 2% UHT processed milk and in a biofilm matrix. Pure cultures at an initial concentration of 10⁵ / ml were used. Heat resistance was determined by two methods. One method was sealed borosilicate glass TDT tubes that were completely submerged in the heating menstrum. Biofilms were …
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Effect of Exopolysaccharides on Pseudomonas aeruginosa Physiology
Bacteria form multicellular aggregates called biofilms. Cells in biofilms are encased by aself-produced matrix composed of proteins, exopolysaccharides (EPS), nucleic acids, and lipids. Biofilm matrix composition mediates how bacteria interact with one another and their environments. The biofilm …
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Elucidating the Molecular Pathways Involved in the Fitness and Water Stress Response of Pseudomonas synxantha 2-79 in the Plant Rhizosphere
… is underpinned by the formation of hydrating biofilms and the utilization of root exudates that contain plant-derived osmoprotectants called quaternary ammonium compounds (QACs). We tested this hypothesis by identifying waters stress response pathways in the 2-79 genome and then constructing a …
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Modeling of gene regulatory networks controlling cell-fate decisions in Bacillus subtilis
… phosphorylated Spo0A (Spo0A~P) activates biofilm formation by indirectly inducing matrix production in a subpopulation of cells via a SinI-SinR-SlrR regulatory network. When the nutrient is further depleted, Spo0A~P activates sporulation by directly and indirectly regulating sporulation …
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Characterisation of the localisation and function of the Bacillus subtilis YuaB protein during biofilm formation
… the community. One type of such community is the biofilm, which is composed of microbial cells enclosed in a biopolymeric matrix. Such biofilms can be formed in a large range of environments from sea beds to animal tissues. Bacillus subtilis is a soil dwelling Gram-positive rod that was shown to …
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An investigation of biosurfactant-mediated disruption and biological activity in mature biofilms formed on plastic surfaces by LPS mutants of Salmonella enterica serovar Typhimurium
… a major foodborne pathogen. Its ability to form biofilms (a community of microorganisms) contributes to contamination in the food industry and clinical environments, posing challenges for disinfection and increasing the risk of spread. Biosurfactants, compounds produced by microorganisms, can …
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The Role of the Extracellular Matrix components of Salmonella enterica serovar Typhimurium Biofilms in Biofilm Formation and Architecture via in vitro analysis
Biofilms are aggregates of bacterial cells enclosed within a self-generated extracellular matrix, forming a three-dimensional structure that allows the bacteria cells to firmly attach to both living and non-living surfaces. The extracellular matrix of Salmonella enterica serovar Typhimurium (STm) …
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Streptococcal colonization of host mucosal surfaces: a study of biofilm formation and dispersal
… Experimental evidence suggests that GAS forms biofilms during the colonization of a surface. A biofilm is a bacterial sessile community encased in an extracellular matrix and attached to a substratum or interface. Biofilms are inherently tolerant to host defenses and antibiotic therapies and …
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In vitro evaluation of equine bone-marrow derived mesenchymal stromal cells to combat orthopedic biofilm infections
… and horses. Treatment failure is often due to biofilms that are communities of bacteria that are adhered to a surface or to each other and are surrounded in a self-secreted extracellular matrix. The biofilm matrix protects the indwelling bacteria from being killed by antibiotics and the immune …
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Effect of iron and haemoglobin on Staphylococcus aureus biofilms
The biofilm infection of Staphylococcus aureus is one of its most common and ‘silently’ harmful virulence traits, leading to endocarditis, central line-associated bloodstream infections, ventilator associated pneumonia, implant-related infection and surgical site infections among others. Biofilms …
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The Development of Substrates and Inhibitors of the PNAG Biosynthetic Machinery
Biofilms are surface-attached colonies of bacteria that are encapsulated in an extracellular matrix composed of a milieu of proteins, nucleic acids and polysaccharides. In a wide variety of clinically relevant bacteria including Staphylococcus epidermidis and Escherichia coli the composition of the …
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The Response of Staphylococcus aureus to Culture in a Low-Fluid-Shear Environment
… the formation of a novel attachment-independent biofilm in which the S. aureus cells were completely encased in an extracellular matrix that conferred increased antibiotic resistance. Further analysis of the cells within the biofilm revealed a decrease in carotenoid pigmentation and growth, an …
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Pasteurella multocida biofilm formation, and the interrelationship of P. multocida with Histophilus somni in a polymicrobial biofilm during bovine respiratory disease
… (BRD) and avian cholera may be associated with biofilm formation. Biofilm formation was inversely related to capsule production (determined by uronic acid and N-acetylglucosamine assays), and was confirmed with capsule-deficient mutants of mucoid strains. Capsule-deficient mutants formed …
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Controlling Pseudomonas aeruginosa Persister Cells by Human Granulocyte Macrophage Colony-Stimulating Factor
… in humans by entering dormancy and by developing biofilms. These mechanisms allow bacteria to exhibit antibiotic tolerance and relapse to an active virulent state when the antibiotic treatments are discontinued. During bacterial invasions, the host immune cells secrete special signaling proteins, …
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Role of Exopolymeric Substances in Biofilm Formation on Dairy Separation Membranes
… and affects product quality. The growth of biofilms on membranes contributes to biofouling. Biofilms are very difficult to eliminate, and will serve as the seed for additional biofilm development and product contamination. The biofilm matrix is composed mainly of microbial exopolymeric …
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Role of Extracellular Polymeric Substances (EPS) on Biofilm Disinfection in a Model Drinking Water Distribution System
Biofilms are undesirable but ubiquitous in drinking water systems. This study investigated the role of extracellular polymeric substances (EPS) in the biofilm life cycle, including planktonic cells, attached biofilm, detached biofilm clusters and redistributed biofilm, in a model distribution …
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Understanding the role of TapA in the formation of <i>B. subtilis</i> biofilms: a biochemical and genetic analysis
… them from their free living planktonic forms. Biofilm formation is an example of such a multicellular behaviour which is widespread in the prokaryotic world. Biofilms can take diverse forms but this work focusses on the surface-attached biofilm formed by the organism Bacillus subtilis. This …
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