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Showing 1 to 20 of 81 for “"Bioconjugation"”.
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Palladium reagents for bioconjugation
… aqueous buffer, offering an efficient method for bioconjugation. Using this strategy, peptides can be rapidly functionalized with small molecules to prepare modified aryl thioether sidechains. Additionally, peptide-peptide and peptide-protein ligations are demonstrated under dilute aqueous …
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Organometallic Palladium Reagents for Polypeptide Bioconjugation and Macrocyclization
Chapter 1: Bicyclic peptides have been developed to mimic good binders that structurally resemble antibody mimetics. The identity of the linker plays a critical role in enforcing the structure of the cyclic entity. Palladium-mediated arylation has enabled cysteine-specific modifications with a …
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Protein Synthesis and Bioconjugation for Design of Antimicrobial Conjugates
New antimicrobial approaches are needed to combat the threat of antibiotic resistance. Gram- negative bacteria are of significant clinical concern, in part due to their cell wall structure that restricts the transport of most antibiotics. Here we describe the development of two chemical strategies …
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Reactive Peptides for Site-Selective Cysteine and Lysine Bioconjugation
Achieving catalyst-free, site-selective modification of proteins in water is a significant challenge in chemical biology. Issues of residue specificity, site-selectivity, reagent stability, and reaction rate are pervasive. To address these challenges, we developed a matched peptide pair termed the …
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Development of a Novel Bioconjugation Platform for the Generation of Homogenous Antibody-Drug Conjugates
… effort has been invested in the development of bioconjugation methods which yield homogenous ADCs with stable linkages and well-defined modification sites. However, despite many recent advances in the field, significant limitations remain. For instance, many methods necessitate the use of …
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Synthesis of fragments of the capsular polysaccharide of <i>Neisseria meningitidis</i> (serogroup A) suitable for bioconjugation
One of the causative agents of meningitis is the gram-negative bacteria Neisseria meningitidis. In the region known as the African meningitis belt, N. meningitidis serotype A is endemic in the population and is also known to cause large scale epidemics. An important virulence factor of this …
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Bioconjugation of aminated DNA as a method of rapid polymer library generation for Corona Phase Molecular Recognition
An experimental study was performed to determine the effects on Corona Phase Molecular Recognition (CoMoRe) of bioconjugating a host of small molecules to DNA wrapped single-walled carbon nanotubes. In addition, the study observed the effects of the DNA sequence length on the subsequent …
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Application of silicon photonic microring resonators to the study of surface bioconjugation and protein capture agent binding kinetics
… consumption. The efficiency of this surface bioconjugation at various pHs and in the presence or absence of catalytic aniline was directly evaluated using silicon photonic microring resonators. It is found that aniline significantly increases the net antibody loading for surfaces …
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Single-Payload Bioconjugation to Native Antibodies And A General Method for the Self-Immolative Release of Amide-Containing Molecules
… a significant challenge. Chapter 1 describes the bioconjugation optimisation of the third generation of tetra-divinylpyrimidine (TetraDVP) linkers. These linkers allow for the creation of single-payload antibody conjugates by re-bridging all four interchain disulphide bonds in IgG1 antibodies with …
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Selective Protein Functionalisation at Methionine
The development of novel bioconjugation strategies for the functionalisation of polypeptides and proteins has been of great benefit to the fields of chemical biology and medicine. Selective protein functionalisation has enabled the development of technologies such as targeted drug delivery, enzyme …
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The Development of Novel Radioimmunoconjugates for the PET Imaging and Radioimmunotherapy of Cancer
… separated into three different categories: 1) bioconjugation strategies, 2) immunoPET imaging, and 3) radioimmunotherapy. This dissertation will attempt to comprehensively cover each of these categories through a series of studies, protocols, and reviews. For the bioconjugation strategies, we …
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Modification of Biopolymers Using Palladium Oxidative Addition Complexes
… and the recent developments in Pd-mediated bioconjugation are provided as well to give context for the following chapters. Chapter 2: Amphiphilic Biaryl Monophosphine Ligands by Regioselective Sulfonation Amphiphilic ligands are valued for their ability to facilitate organometallic reactions …
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Multifunctional Chemical Cross-linkers for Drug Carriers and Molecular Probes
… biomedical imaging and drug delivery. Broadly, bioconjugation is the use of a chemical cross-linker to covalently attach non-natural reporters, chemical handles, drug cargo, or targeting ligands to biologics to augment their innate properties and functions. Many bioconjugation strategies attach …
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Development of a New General Click Chemistry and Applications in Bioconjugation: Part I: Rewiring Bacteria Cell Surfaces with Bio-Orthogonal Chemistry Part II: A Novel General Dialdehyde Click Chemistry for Primary Amine Conjugation
… of chemical research ranging from polymers, bioconjugation to material science and nanoscience
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Carbon Monoxide Prodrugs for Targeted Cancer Therapy
… peptide synthesis and subsequent site-specific bioconjugation to therapeutic antibodies. Trastuzumab–CO-prodrug conjugates were synthesised with CO-to-antibody ratios of up to an average of 23 and demonstrated tumour-specific CO release in HER2-overexpressing cells. The platform was subsequently …
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Peptide conjugation to enhance oligonucleotide delivery
… conjugates. Using the judicious choice of bioconjugation chemistry, highly-active modular constructs were synthesized that contain three peptides linked to one oligonucleotide. In addition to CPPs for oligonucleotide delivery, one section of this thesis employed perfluoroaryl macrocyclic …
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Driving forces of self-assembly in protein-polymer bioconjugates
… with a diverse range of applications. Bioconjugation to a polymer allows the protein to maintain or even enhance its activity while imparting self-assembly capabilities to the overall material, which provide control over the orientation and nanostructure of the bioconjugates, enabling …
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Encapsulamento de nanopartículas magnéticas em polímeros acrílicos e avaliação de hipertermia para potencial tratamento de câncer
… respectively. Besides, the folic acid bioconjugation with the surface of polymeric particles obtained by inverse miniemulsion was performed and evaluated. The low coercivity and remanence of magnetic nanoparticles, as well as the superparamagnetic behavior at room temperature, favor …
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Chain-end functionalization and modification of polymers using modular chemical reactions
… tuning, chromatography and bioconjugation (enzyme thermoprecipitation and recovery, improvement of enzyme activity). Different α-functionalized co/polymers containing cholesterol moiety, aldehyde, t-Boc protected amine, TMS-protected alkyne and NHS-activated ester were …
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