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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Bioconjugates"”.
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Vitamin B12 Bioconjugates In Pharmaceutical Design
<p>Abstract</p> <p>This thesis addresses several questions focused on the vitamin B12 (B12) dietary uptake pathway, in particular that of the enteric B12 transport protein intrinsic factor (IF) and its receptor cubilin, and the expression and exploitation of IF/cubilin in pharmaceutical …
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Targeting GRP78 in Cancer with Nucleic Acid Bioconjugates
<p>Nucleic acid bioconjugates have gained widespread use in medicinal chemistry research programs aimed at fighting human malignancies such as cancer, diabetes, genetic and infectious diseases. Their popularity stems from their ability to accelerate the drug development process by conjugating …
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Predicting self-assembly in globular protein-polymer bioconjugates
Globular proteins offer powerful solutions for addressing challenges in the fields of medicine, industry, defense, and energy. Enzymes perform reactions with high efficiency and specificity, allowing for minimal generation of undesired side products even while exhibiting rapid turnover-traits …
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Driving forces of self-assembly in protein-polymer bioconjugates
Protein-polymer bioconjugates have shown great promise as high-performance biomaterials, with a diverse range of applications. Bioconjugation to a polymer allows the protein to maintain or even enhance its activity while imparting self-assembly capabilities to the overall material, which provide …
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Synthesis, characterization and biological studies of carboranyl-porphyrin bioconjugates for treatment of brain tumors
<p>Chapter 1 gives a brief introduction to brain tumors, approaches to deliver drug across the blood brain barrier (BBB), boron neutron capture therapy (BNCT) for brain tumors, application of carboranyl derivatives in attempt to treat brain tumors using BNCT. Chapter 2 involves synthesis, …
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PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage
… of new nuclease mimics on the basis of PNA-metal bioconjugates is described. The design of artificial enzymes for sequence-specific DNA and RNA cleavage is one of the most challenging problems in modern biotechnology, since the commercially available biotools are restricted to a limited number of …
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Design, synthesis, and evaluation of insulin bioconjugates for the application of enhanced basal and glucose-responsive activity
Since its discovery by Banting and Best, the administration of exogenous insulin to control blood glucose levels has been a primary method of treatment for severe cases of diabetes mellitus. Several decades of insulin engineering and development has led to the clinical introduction of two broadly …
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Multifunctional Chemical Cross-linkers for Drug Carriers and Molecular Probes
Bioconjugates are an indispensable tool for molecular biology as well as an established modality for biomedical imaging and drug delivery. Broadly, bioconjugation is the use of a chemical cross-linker to covalently attach non-natural reporters, chemical handles, drug cargo, or targeting ligands to …
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Solid Phase Synthesis and Biological studies on metal conjugates of bioactive peptides for targeted delivery
… characterisation of the synthesized bioconjugates was carried out by various techniques such as NMR, RP-HPLC, MS and electrochemistry. The cellular uptake and nuclear localisation of the metallocene-NLS and -TAT bioconjugates was monitored by fluorescence microscopy in living liver …
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Controlling nanostructures of globular protein-polymer block copolymers in bulk solutions and in thin films
… of globular protein-polymer block copolymers. Bioconjugates composed of a model red fluorescent protein, mCherry, and a synthetic homopolymer with different chemical moieties are synthesized. Modulated interactions between protein and polymer by introducing polymer blocks with different …
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Synthesis and Cytotoxicity Studies of Gold Nanoparticle Systems
… The colloidal gold nanoparticles, MPCs and bioconjugates were further investigated for inherent biologic effects through a series of end-point based in vitro assays. The cytotoxicity, namely the causation of necrotic cell death was studied using the neutral red assay on CHO22 cell line. All …
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Elastin-like polypeptide (ELP) tags for self-assembly and high-throughput processing of functional protein materials
… Self-assembly of globular protein-polymer bioconjugates into nanostructured materials is an attractive protein immobilization strategy that allows for both high protein packing density and control over protein orientation in the material. However, challenges associated with protein-polymer …
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Optimized Peptide Nanomaterials as Delivery Vehicles for Hydrophobic Metal-Based Anticancer Agents
… may lead to the formation of new peptide-drug bioconjugates with increased activity and selectivity. The physiological stability of these peptide delivery vehicles has been optimized by capping the N-terminus with an acetyl group. This simple backbone modification was shown to not prevent …
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The synthesis and characterisation of inorganic and organic luminophores suitable for biomolecule conjugation
… novel ruthenium and iridium luminophores, their bioconjugates and nanoparticle conjugates which were prepared for applications in cell imaging. A key aim of this thesis was the synthesis, characterisation and identification of novel bioconjugates suitable for applications in cellular imaging. …
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Stimulated emission from biological materials and novel optical resonators for diagnostic and imaging applications
… of a FRET-based laser using biotin-streptavidin bioconjugates comprising organic dye and gold nanoparticle donor/acceptor configurations. Furthermore, we introduce an optical resonator which can be fabricated from the directed assembly of a particle monolayer and is capable of producing hundreds …
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Exendin 4 Conjugation and Sequence Modification to Treat Type 2 Diabetes and Obesity
… drug exendin 4 (Ex-4), and vitamin B12 (B12) bioconjugates, thereof.</p> <p>Q1. (Chapter 2) What effect does B12 conjugation to Ex-4 have on agonism of the glucagon-like peptide-1</p> <p>receptor (GLP-1R) in vitro and on PD/PK (including brain uptake and function) in vivo?</p> <p>Goal: To …
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Synthesis and Characterisation of Photoresponsive Azobenzene-Biomolecule Conjugates
… photophysical properties of a selected range of bioconjugates has been explored: the photostationary states were determined using NMR spectroscopy and the kinetic parameters of the cis to trans relaxation process determined by a UV spectroscopy study. The experimental details are reported in …
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Design and synthesis of novel compounds as fatty acids binding protein inhibitors and as gallium-68 chelators for positron emission tomography
… second one, the design of two targeting peptide bioconjugates for the detection of cancers is reported. Fatty acid binding proteins are a class of proteins involved particularly in the transport of fatty acids in human. Recently it comes out that the Fatty acid binding proteins are an interesting …
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Synthesis of derivatizable semiconductor nanocrystals through rational ligand design, and applications thereof
… thermal activation, with thiols to form stable bioconjugates.
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Synthesis, characterization and biological investigation of self-delivering and modified short interfering RNAs (siRNAs)
… This dissertation reports the use of two bioconjugates, cholesterol and folic acid, to improve the cellular uptake and delivery of siRNAs and explores the incorporation of a novel sugar moiety within siRNAs to assess its effect on gene-silencing activity. Cholesterol has been extensively …
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