Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"Biochemical investigation"”.
-
A biochemical investigation of actin disassembly mechanisms
The dynamic nature of the actin cytoskeleton enables the rapid shape changes that are necessary for processes such as wound healing, motility and division of cells. Disassembly of actin filaments is extremely critical for the reorganization of cell shape. The cell possesses several factors that …
-
Biochemical investigation of the ubiquitin carboxyl-terminal hydrolase family
<p>The proteasome is the machinery in eukaryotic cells that degrades protein and recycles the amino acids. Protein degradation is a highly regulated process which starts by the attachment of chains of ubiquitin, which serves as a tag that marks a protein for degradation. This function involves the …
-
Structural and Biochemical Investigation of Fanconi Anemia Pathway Activation
… been thoroughly studied using genetic, cellular, biochemical, and structural tools. FANCI phosphorylation by the ATR DNA damage kinase stimulates D2-I ubiquitination in cells, which enables timely and coordinated FA pathway activation to ensure DNA repair fidelity. In this dissertation, I …
-
Glycans in host-pathogen interactions : an integrated biochemical investigation
The epithelial cell-extracellular matrix interface primarily comprises of complex glycans and glycoconjugates. The widespread distribution of these glycans on the epithelial cell surface makes them ideal targets for interaction with microbial pathogens. In this thesis, a framework of integrated …
-
Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… recognise poly(A). In Chapter 2, structural and biochemical studies reveal that these DEDD-family deadenylases recognise the intrinsic stacked, helical structure of single-stranded poly(A) RNA. Recognition of this conformation in different biological contexts, enabled by the unique …
-
Biochemical investigation into the toxic salivary secretion of the tick, Ornithodoros savignyi Audouin (1827)
… results reported. Sand tampan ticks used for the investigations were collected in the North West Cape Kalahari with traps baited with solidified carbon dioxide (S 5), Oral secretions of the ticks were obtained by parasympathetic stimulation as described by Howell (S 6), Some properties of the …
-
Biochemical Investigation of the Intracellular Trafficking of Non-Viral and Hybrid Gene Therapy Vectors
We also used reverse transcriptase inhibitor 3'-azido-3'-deoxythymidine (AZT) applied of post-transfection time increments to determine the timeframe of reverse transcription, thereby estimating endosomal escape of VLP. MLV were observed to achieve reverse transcription rapidly, with more than half …
-
Biochemical investigation of phosphodiesterase type IV post-translational modification, cellular localisation and interaction with associated binding proteins
cAMP is a secondary messenger that is involved in a variety of signalling pathways through its effectors including EPAC, PKA and ion channels. cAMP signalling regulates processes such as memory, muscle contraction and inflammatory responses. PDE enzymes offer a mechanism to negatively regulate …
-
A biochemical investigation into aspects of the neuro active amino acid receptor complexes in the insect nervous system
Biochemical investigations were undertaken to study the glutamate (L-glu) and aminobutyric acid (GABA) receptors in the central nervous system (CNS) of the American cockroach, Periplaneta americana. The aim was to provide a simple, quick and cost effective method of screening new chemical entities …
-
Genomic and biochemical investigation of soybean antioxidant metabolism in response to growth at elevated carbon dioxide and elevated ozone
Metabolism in an environment containing of 21% oxygen has a high risk of oxidative damage due to the formation of reactive oxygen species. Therefore, plants have evolved an antioxidant system consisting of metabolites and enzymes that either directly scavenge ROS or recycle the antioxidant …
-
Structural and Biochemical Investigation of the Molecular Mechanisms of DNA Response and Repair in Humans and <em>Escherichia coli</em>.
<p>The genomes of all living cells are under constant attack from both endogenous and exogenous agents that damage DNA. In order to maintain genetic integrity a variety of response pathways have evolved to recognize and eliminate DNA damage. Replication protein A (RPA), the eukaryotic …
-
Characterisation of trimethoprim resistance transposons and their gene products
… resistance of greater than 1000 ug/ml. Biochemical investigation of the transposon gene product revealed a close resemblence between the trimethoprim resistant dihydrofolate reductase (DHFR) of Escherichia coli J62(RP4::Tn4135) and that of the type I plasmid enzyme, encoded by RP4::Tn7_. …
-
The iron-binding proteins of iron-absorbing rat intestinal mucosa
… topic of iron absorption has received intensive investigation over a relatively long period of time. Most of the investigative thrust has come from clinical medicine and allied fields, with some associated biochemical investigation. Evidence from the latter has pointed towards the involvement of …
-
Biochemical characterization of the E. coli Very Short Patch Repair pathway and its coordination with methyltransferase repair of 0⁶-methylguanine
… complete the repair event. However, until now, biochemical investigation of the repair steps downstream of Vsr incision have been lacking. Herein, we describe two novel in vitro assays used to probe the biochemical events of VSPR. The first was used to verify the reconstitution of VSPR using …
-
A functional role for transfer RNA-derived microRNAs in human B cells
… a diverse repertoire of small RNAs. Genetic and biochemical investigation of these molecules has demonstrated that many small RNAs, most notably the microRNA subclass, possess the capacity to influence the expression of other genes, adding substantial complexity to our understanding of genetic …
-
Characterisation of glycosidase enzymes of wine lactic acid bacteria
… of research were followed in this study: a) a biochemical investigation of glycosidase activities using artificial (p-nitrophenol- linked) substrates, b) an investigation of the enzymatic effect of LAB on wine anthocyanins, and c) identification and cloning of a putative β-glucosidase gene from …