Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Biochemical engineering"”.
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Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
… study focuses on the application of metabolic engineering techniques to diminish acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of plasmid encoded cadA-LacZ fusion protein with $\beta$-Galactosidase activity …
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Application of metabolic and biochemical engineering techniques to enhance metabolic flux throughput and improve succinate production in Escherichia coli
Using biochemical engineering principles and metabolic engineering tools in tandem can considerably improve process productivity by manipulation of the throughput of certain pathways. Escherichia coli was chosen as the model system because of its vast available genetic information, rapid growth and …
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Applications of motif discovery in biological data
… difficult problem in computational biology and biochemical engineering. The most prevalent example of such a problem is finding conserved DNA sequences in the upstream regions of genes that are believed to be coregulated. Other examples are as diverse as identifying conserved secondary structure …
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A Study of Polymer-Small Molecule Interactions for Solid-Liquid Two Phase Partitioning Bioreactors With Emphasis on the Bioproduction of 2-Phenylethanol
Biphasic systems have been studied for in situ product removal (ISPR), and have shown improvements in bioreactor performance. With immiscible solvents, concerns associated with solvent biocompatibility, bioavailability and operation have been identified. One alternative is a solid-liquid system in …
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Characterisation of the structural stability of transketolase under biocatalytically relevant conditions
The enzyme transketolase (TK; E.C. 2.2.1.1) from Escherichia coli occupies a pivotal place in metabolic regulation. TK catalyses the interconversion of sugars by transferring a two-carbon ketol unit from a ketose donor substrate to an aldolase acceptor substrate. It is also an important biocatalyst …
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Characterisation of chromatography adsorbents for antibody bioprocessing
Currently the purification of monoclonal antibodies for therapeutic purposes is reliant on protein A affinity chromatography. The rapid growth of this class of therapeutic and their high value makes the understanding of protein A chromatography an important target. There is a range of commercially …
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Centrifugal recovery of embryonic stem cells for regenerative medicine bioprocessing
… an understanding of the the impact of the engineering environment on ES cells is sought. This thesis uses murine ES cells as a mimic for ES cell types that will be used in cell based regenerative medicine applications to examine the bioprocessing impact of centrifugal recovery cells. A …
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Tube-forming device design for the creation of cell-integrated alginate tubes
… different purposes – from cell therapy to tissue engineering. The aim of this work has therefore been on the ability: to reproducibly create alginate tubes with uniformly thick walls of predictable thickness; to be able to monitor and quality control said tubes and a generic cell suspension, …
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The development and application of chemometrics to process analysis in an industrial environment
This thesis describes two main sections of work, an examination of a commercial product, Intrasite Gel, and the development of an algorithm for variable selection using projected latent structures.Following on from the successful development of a variable selection procedure for multivariate linear …
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The extraction of Cytochrome C and DsRed2 into reverse micelles
Cytochrome c and DsRed2 were successfully extracted into reverse micelles by the contacting of an aqueous protein-containing phase with an organic phase. Two important properties that differentiate the extraction profiles of these proteins are pI and size. Cytochrome c is a relatively small, …
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Chemical and biological modification of porous silicon photonic crystals.
Porous silicon (PSi) photonic crystals have aroused research interest as label-free chemical and biological sensing transducers owing to the ease of fabrication, high quality optics and a sensitive optical response to changes in efractive index. A major impediment to using PSi materials as sensors …
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An evaluation of the environmental fate of reactive dyes
Dyestuffs are widely used industrial chemicals, yet surprisingly little is known about their fate in the environment. The potential modes of transformation and removal of reactive dyes in treatment and in the environment are principally through anaerobic and aerobic biodegradation and …
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Reassessment of Biowish Activation Procedure for Denitrification
<p>BiOWiSH<sup>TM</sup> – Aqua is a blend of preserved multi-bacteria culture with the capability of denitrification. If an anaerobic nitrate rich activation procedure is used instead of the standard aerobic activation procedure, the denitrification rate is increased by 28 percent under the …
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Detection Method of Subclinical Atherosclerosis of the Carotid Artery with a Hemodynamics Modeling Approach
… and carotid intima media thickness, therefore an engineering detection model based on quantifiable data is needed. Laminar and turbulent flow, dictated by Reynolds number and relative roughness, was modeled through the carotid artery bifurcation to compare shear stress and shear rate. …
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A Rubric for Electrochemical Testing of Metallic Biomaterials
<p>Corrosion is a major factor for the failure of metallic medical implants. Testing a metal’s suseptability to corrosion prior to implantation is key to a successful implantation. Electrochemical processes were used in this study to evaluate the characteristics of corrosion of both AISI 316 …
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ST Monitoring on the Programmer for Implantable Cardioverter Devices
Cardiovascular disease is one of the most prevalent causes of death which has a high mortality rate. If identified early and treated appropriately, the mortality in patients with cardiovascular disease can be hugely reduced. For several decades, 12-Lead ECG has been the standard technique used to …
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Hydrodynamic Focused Passive Separation Under Continuous Flow in a Microfluidic Chip
<p>A continuous flow, passive separation device was designed using an equivalent circuit to create variable flow rates for hydrodynamic focusing to drain channels and collection outlets. By varying the diameter of the sample inlet connection into the reservoir, the particle position was influenced …
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Investigation of Bacillus subtilis as a Biopesticide Against Botrytis cinerea
<p>The objective of this thesis was to investigate BiOWiSH<sup>TM</sup>-Aqua, a commercial dry solid formulation containing a consortium of bacteria and yeast, as a biopesticide for treatment of <em>Botrytis cinerea</em>, a gray mold that affects strawberries. BiOWiSH<sup>TM</sup>-Aqua was compared …
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