Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 157 for “"Biochemical Assays"”.
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Structural and Functional Studies of Nucleoside Transporters
… mechanisms using X-ray crystallography and biochemical assays. We solved multiple crystal structures of CNT along its transport cycle at 3.45-4.2 Å, which provide novel insights into the elevator-type transport mechanism of secondary active transporters.</p>
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EXPANDING THE POTENTIAL PRENYLOME: PRENYLATION OF SHORTENED TARGET SUBSTRATES BY FTASE AND DEVELOPMENT OF FRET-BASED SYSTEM FOR DETECTING POTENTIALLY “SHUNTED” PROTEINS
… C-terminal sequences using both genetic and biochemical assays. Surprisingly, biochemical assays utilizing purified mammalian FTase and Cxx substrates reveal prenyl donor promiscuity leading to both farnesylation and geranylgeranylation of these sequences. The work herein expands the …
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Development of Dendrimer-based Technologies for Phosphoproteomics and Glycoproteomics in Biomarker Discovery
… and quantification of PTMs using several biochemical assays and mass spectrometry-based shotgun proteomics.
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Physical analysis of collagen-GAG composite scaffolds for nucleus pulposus tissue regeneration
… acid (HA). The scaffolds were analyzed using biochemical assays to determine final composition. Chemically cross-linked scaffolds were analyzed to determine pore size and cross-link density. It was determined that every material type contained large enough pore size (275 gm) to seed nucleus …
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Η επίδραση της εγκυμοσύνης στην ανάπτυξη λιθίασης των χοληφόρων: Προοπτική μελέτη
… biliary tract, pancreas and kidneys, as well as biochemical assays as bilirubin, transaminase, alkaline phosphatase, LDH, serum amylase, cholesterol, triglyceridia. Two women had asymptomatic cholelithiasis at the beginning of the pregnancy (0,6%). Six women developed cholelithiasis after the …
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Developing osteoarthritis treatments through cartilage tissue engineering and molecular imaging
… the same properties as native cartilage tissue. Biochemical assays and imaging techniques can be used to study some of the main components of cartilage and assess the value of potential therapies. Agarose and self-assembling peptides have been used to make hydrogels for in vitro culture of bovine …
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Molecular Modulation of a-Subunit VISIT-DG Sequence Residue Asp-350 in the Catalytic sites of <em>Escherichia coli</em> ATP Synthase.
… by site directed mutagenesis and several biochemical assays were performed on them to understand the role played by the amino acid residues in Pi binding. Biochemical results suggest that αD350 may be involved in catalysis of ATP synthase and play an important role in Pi binding, whereas …
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Discovery and characterization of novel RNA repair systems
… mutants for this new ligase and carried out biochemical assays. These studies provide additional insight into the mechanism of RNA ligation by Pnkp/Hen1. Based on a comprehensive Blast search, a novel RNA repair system composed of three proteins (Pnkp1, Rnl and Hen1) has been found in 10 …
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Studies on the antioxidant effects of the extract of Herba leonuri, a traditional Chinese medicinal herb, on ischemic rat myocardium after acute myocardial infarction
… 1d, 1w, and 3w) to obtain left ventricles for biochemical assays. Our study demonstrates for the first time that HL does have antioxidant effects both in vitro and in vivo (in an animal model of myocardial infarction). The antioxidant effects of HL is exerted only under the condition of …
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Role of a-Subunit VISIT-DG Sequence Residues Ile-346 and Ile-348 in the Catalytic Sites of Escherichia Coli ATP Synthase.
… Each residue was mutated to A/R/D/Q. Growth assays in limiting glucose media and on succinate plates suggests αIle-346 and αIle-348 are critical for the normal enzymatic function (oxidative phosphorylation). And the biochemical assays do suggest both αI-346 and αI-348 are required to maintain …
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Mechanical and biochemical properties of human cervical tissue
… presents an investigation of the mechanical and biochemical properties of cervical samples from human hysterectomy specimens. Three clinical cases were investigated: non-pregnant hysterectomy patients with previous vaginal deliveries, non-pregnant hysterectomy patients with no previous vaginal …
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De Novo Initiated RNA Synthesis by the Hepatitis C Virus RNA-dependent RNA Polymerase
… that are annealed stably to a template RNA in biochemical assays. The closed conformation of NS5B as per X-ray crystal structures can only accommodate a ssRNA but not a dsRNA, hence necessitating a conformational change between de novo initiation and elongation. The details of these …
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Characterization and Development of Strategies for Altering Protein Expression in JSL1 Cells
… under both resting and stimulated conditions. Biochemical assays have shown that the regulatory protein hnRNP L binds to this silencer sequence and results in basal exon repression during resting conditions and undergoes modifications which further influence exon skipping upon stimulation. …
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Diagnostic characterization of Malassezia pachydermatis isolates from canine otitis externa
… MS) identification was used, and common biochemical assays were conducted. A subset of isolates could not be identified using the MALDI-ToF MS standard library and had different colony morphologies than the M. pachydermatis type strain, ATCC 14522. DNA sequencing the ITS region and …
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Synthesis and Design of ClpP Activators as Novel Antibiotics
… by using minimum inhibitory concentration assays and in vitro biochemical assays for ClpP activation. Explore their potential as antibiotics through mitochondrial toxicity tests, glucose/galactose assays, and biophysical measurements related to metabolism and clearance, including thermal …
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Investigating and expanding the functionality of RNA catalysts: Studies of the hepatitis delta virus, the hammerhead, and the aminoacyl-tRNA synthetase-like ribozymes
… phosphodiester cleavage reaction. Biochemical assays and X-ray crystallography were used for probing the active site of two small self-cleaving ribozymes, the hepatitis delta virus (HDV) ribozyme and the hammerhead ribozyme. Results presented here suggest that divalent metal ions …
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Modulation of Alpha-Subunit VISIT-DG Sequence Residues Ser-347, Gly-351 and Thr-349 in the Catalytic Sites of <em>Escherichia coli</em> ATP Synthase.
… via site directed mutagenesis. Results from biochemical assays showed that αSer-347 is required for transition state stabilization and P<sub>i</sub> binding whereas αGly-351 is only indirectly involved in P<sub>i</sub> binding and most likely maintains structural integrity of the catalytic …
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Prediction of Articular Cartilage Remodeling During Dynamic Compression with a Finite Element Model
… of cartilage microstructure were obtained by biochemical assays and quantified polarized light microscopy. Significant differences were measured between the GAG content in the inner and outer portions of the sam- ples stimulated with dynamic unconfined compression. The COL fiber network was …
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Yeast Ataxin-2, A Protein with Many Friends: Identification of a Novel Role for Pbp1 in Mitochondrial Biogenesis
… respiration. Utilizing genetics and biochemical assays, I discovered that loss of Pbp1 leads to decreased Puf3-specific mitochondrial proteins, suggesting a functional relationship between Pbp1 and Puf3. I also found that Pbp1 stabilizes and promotes Puf3-target mRNAs in respiratory …
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Development of microfluidic devices to maintain and interrogate tissue biopsies - biomedical applications
… viable and functional state for over seven days. Biochemical assays, histological examination, confocal imaging, epi-fluorescent microscopy were all performed to assess the viability of the tissue before and after interrogation by several apoptosis inducing and chemotherapeutic agents. Liver …
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