Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Biochemical Assay"”.
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Biochemical Assay Optimization and Computational Screening Efforts to Identify Potential LuxS Inhibitors
… vitro</em> using an optimized LuxS inhibition assay.</p>
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Design of a micro-interdigitated electrode for impedance measurement performance in a biochemical assay
… on precise electrode performance, such as the biochemical assay in the Daktari CD4 diagnostic system, it is necessary to minimize variation through robust design and quality control. Interdigitated electrode design was investigated to identify design strategies which maximize electrode …
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Characterization of Ebola virus VP35-dsRNA binding for drug development against Ebola virus disease.
… in prokaryotic system and used to validate a biochemical pull-down assay for the screening of small molecules targeted to the VP35-double strand (ds)-RNA interactions. However, low rVP35 amount of purified protein and the use of radioactive substrate for binding evaluation, strongly limited …
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Assays for lycosyltransferases
… was the development of operationally simple assays for GTs and their application for the identification and characterisation of novel GT inhibitors. The <br/>main focus was on the bacterial GT, α1,4-galactosyltransferase C (LgtC) which transfers galactose from uridine-diphosphate-galactose to …
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Instrumentation for a distributed feedback laser biosensor system
… and kinetic scans over time. An example biochemical assay is performed to demonstrate and evaluate the current capabilities of the biosensor system. Plans for the future advancement of the system, including the possibilities of hardware improvements, device fabrication refinements, data …
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Identification and Characterization of the Mechanisms of Action of Novel Antiviral Compounds Targeting the Minimal Replication-Transcription Complex of SARS-CoV-2
… we purified the viral proteins and set up a biochemical assay for the identification and molecular characterization of different classes of SARS-CoV-2 RdRp inhibitors. In collaboration with Dompé Farmaceutici S.p.A., we identified 42 novel non-nucleos(t)ide inhibitors of SARS-CoV-2 RdRp by …
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Inhibition of enzymes from mycobacteria using fragment-based approaches
… fragments were screened using a range of both biochemical and biophysical methods. The resultant fragments showed evidence of weak protein binding. As an alternative strategy, derivatives of the HTS hit were synthesised and screened by a biochemical assay, which identified nanomolar inhibitors …
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Phosphorylation of the replicative helicase by the S-phase kinase, Dbf4-Cdc7, at origins of replication in Saccharomyces cerevisiae
… of the role of DDK in initiation, we developed a biochemical assay to examine the DDK phosphorylation of pre-RC-linked Mcm2-7 complex. We found that DDK specifically targets the MCM complex and is recruited to the origin by the MCM complex. DDK preferentially phosphorylates the MCM complexes most …
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Biochemical and immunological investigations into cystic fibrosis
In an attempt to produce a quantitative biochemical assay which would assist in the detection of individuals heterozygous and homozygous for the cystic fibrosis (CF) gene, two general approaches were adopted. In the first, attempts were made to produce an antiserum specific for the cystic fibrosis …
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Development of biophysical and Mass Spectrometry Assays for APOBEC3 cytosine deaminases: discovery and validation of cnvalent and non-covalent ligands
… on A3B; next, the virtual screening following by biochemical assay screening resulted in the discovery of novel A3B ligands. Chapter 3 discusses a novel approach to A3A ligand discovery through fragment-based disulfide tethering, which yielded several fragments that could prevent DNA binding or …
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Preparation, characterisation and transcriptome analysis of RNA frm human vCJD brains.
… prion protein levels to undetectable by biochemical assay was developed. RNA preparations were made from sample of the frontal parasagital cortex, sub-frontal cortex and cerebellum of 78 human autopsy cases; 21 vCJD, 26 other neurological disease (OND) and 31 nonneurological disease …
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Screening Listeria monocytogenes from bulk tank milk and in-line milk filter samples for the routine monitoring of the dairy farms
… other Listeria species were identified by API biochemical assay and multiplex PCR. The results indicate that milk filters are ideal as a sampling material in detecting L. monocytogenes than BTM samples. However, the presence of L. monocytogenes in milk filters was not associated to the presence …
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Mechanism of Ubiquitin Induced Activation of RIG-I Like Receptors in Antiviral Innate Immunity
… and that of MAVS. Therefore, the underlying biochemical mechanisms of how activation signals are transduced from a cytosolic receptor, RIG-I, to a mitochondria signaling protein, MAVS, is not clear. Protein ubiquitination have pivotal roles in diverse cell signaling pathways, including those …
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THE IMPACT OF ACROLEIN ON LUNG FUNCTION: EXPLORATION OF NITROSATIVE STRESS-INDUCED LUNG INJURY AND THERAPEUTIC APPROACHES
… of lung function, we used a few well-established assays to characterize oxidative stress levels via measurement of glutathione peroxidase activity, nitrosative stress levels by measuring nitrite concentrations, and lipid peroxidation damage by measuring malondialdehyde in bronchoalveolar lavage …
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Polymer-Based Microfluidic Devices for High Throughput Single Molecule Detection: Applications in Biological and Drug Discovery
… is a viable platform for parallel processing of biochemical reactions to increase data production rates due to its ability to generate fluidic networks with a high number of processors over small footprints suitable for optical imaging. Single-molecule detection (SMD) is another technology which …
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Understanding Host Antiviral Signaling Pathways
… Here, I describe the development of an in vitro biochemical assay to detect the activation of a key kinase involved in the activation of IRF3. This assay will be a valuable tool for identifying not only the specific IRF3 kinase, but also other molecular components involved in the antiviral …
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Investigation of Narrow Spectrum Targets in Antibacterial Drug Discovery
… techniques, including structural studies, biochemical assay development and application, and inhibitor screening and testing. Methods: An assortment of drug discovery techniques were employed for the targeting of SmTopoI and CdFabK, including different protein expression and purification …
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Xenopus laevis as a chemical genetic screening tool for drug discovery and development.
… genetic screening, appropriate cell based assays and chemoinformatical analysis we have developed an efficient and effective screening strategy for the rapid identification of hit compounds that are likely to be acting through either well known or novel targets that may have possible …
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Functional dissection of the S. cerevisiae helicase activating factor Cdc45
… for their ability to complement Cdc45 function biochemically. Purified Cdc45 mutants were found to be associated with Hsp70 chaperones, and subsequently failed to fully restore in vitro replication in a Cdc45-dependent biochemical assay. Co-immunoprecipitation studies revealed an interaction of …
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On-line screening of bioactive compounds and application of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry for the analysis of birch bark
… couple on-line chromatographic separation and biochemical detection, are called high-resolution screening methods. In this system, high-performance liquid chromatography separates complex mixtures, and a post-column biochemical assay determines the activity of the individual compounds present …
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