Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"BioID"”.
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
… proximity-dependent biotin identification (BioID), in combination with quantitative proteomic (SILAC) techniques, to identify putative TbRP2-interacting proteins in vivo. A selected cohort of these proteins were subsequently interrogated by bioinformatics, localised within the cell using a …
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Identification of Candidate Effector Proteins from Fusarium graminearum during Infection of Arabidopsis thaliana Using Proximity-Dependant Biotin Identification (BioID)
… biotin identification (BioID) was used to identify potential effector proteins secretedin plantabyF. graminearumduring the infection ofArabidopsisseedlings. BioID analysis revealed over 300 proteins fromF. graminearum, of which 99 were considered to be candidate effector …
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Quantitative Deubiquitinase (DUB) Interactomics to Dissect the Biology of Cancer Associated DUBs
… of USP35, I applied the recently developed BioID method and established a bioinformatics pipeline to capture the isoform-specific interactome of USP35. The high-confidence proximity interacting proteins (HPIPs) obtained from the BioID method revealed that each isoform of USP35 interacts with …
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Characterisation of potential Holliday junction resolvases in Trypanosoma brucei
… of the homologous protein localisation and a BioID analysis to interrogate protein-protein interactions. Bioinformatics analyses identified two proteins with homology to human GEN1, TbFEN1 and TbRAD2. Of these two proteins, TbFEN1 underwent large scale purification and was used for the …
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Assessing Helicobacter pylori's HcpE and DsbK's Effects on Immunostimulation in Host Gastric Cells
… To identify host potential interacting partners, BioID (proximity-dependent biotin identification) and Immunoprecipitation (affinity purification) were implemented, and candidates were identified by mass spectrometry. Finally, the subcellular localization of HcpE in host cells was assessed by …
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Forward and reverse genetic approaches to investigate cellulose biosynthesis in Physcomitrium patens
… a strategy called Biotin identification (BioID), aimed at identifying proximate and vicinal proteins in vivo associated with CESAs in P. patens. I generated multiple BioID-CESA translational fusions by homologous recombination to identify biotinylated proximate proteins. Due to …
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Molekulare Charakterisierung von CP75, einem neuen centrosomalen Protein in Dictyostelium discoideum
… zeigen aber keine Interaktion mit Aktin mit der BioID-Methode. Die N-terminale Domäne von CP75 enthält eine potentielle Plk1- Phosphorylierungssequenz. Die Überexpression der nichtphosphorylierbaren Punktmutante (GFP-CP75-Plk-S143A) ruft verschiedene Phänotypen wie verlängerte oder …
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Innovative local texture descriptors with application to eye detection
… FLBP is demonstrated on eye detection using the BioID and the FERET databases. Experimental results show that the FLBP method significantly improves upon the LBP method in terms of both the eye detection rate and the eye center localization accuracy. As LBP is sensitive to noise especially in …
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A New Approach To Identifying Effectors and Regulators of Small GTPases
… developed in vivo proximity labelling technique BioID was used in this study. Here, BioID was redirected to the ectopic location of the surface of mitochondria (hereafter called MitoID) with the aim of restricting the non-specific background. Applying this method to 25 small GTPases of the Ras …
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Mechanisms of cell adhesion regulation by herpes simplex virus
… spread or survival. Biotin-proximity ligation (BioID) was used to identify novel viral and cellular protein interaction partners for pUL21 and pUL7:pUL51. The IPP complex, which consists of integrin-linked kinase (ILK), PINCH and parvin, was identified as a potential interaction partner for …
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Characterisation of LITAF, a protein associated with Charcot-Marie-Tooth disease type 1C
… assay and the in-situ proximity labelling assay, BioID. A number of novel potential binding partners were identified in both assays. Among the list of potential binding partners, BAG3 was captured in both pull-down assays and was chosen for further studies. The interaction with LITAF was …
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Discovery and functional analyses of new components of the apical complex and the pellicle of Toxoplasma gondii
… proximity-dependent biotin identification (BioID) was applied using AAP6 as bait. Two novel annuli proteins, which may be related to vesicle trafficking, named TgSnare and TgSyntaxin were discovered from the BioID study. Collectively, these studies tell us for the first time that the annuli …
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Post-Translational Regulation of Zeb1 Contributes to Tgfβ-Mediated Emt
… We also expose novel Zeb1 interactions using the BioID method for proximity-dependent labeling of proteins. These results lead us to hypothesize that by targeting factors which mediate Zeb1 post-translational modification we may provide a therapeutic approach for metastasis suppression in …
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The PLK1 and NLRP3 interactome in NLRP3 inflammasome activation
… Using proximity-dependent biotin identification (BioID), a method based on fusion of a promiscuous biotin ligase to a protein of interest, I identified 553 and 645 proteins as potential new interacting partners of NLRP3 and PLK1 respectively during inflammasome activation. Using this method, I …
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The Role of HOIL 1 Ubiquitin Ligase Activity in MDA5 Antiviral Signaling
… in a distinct and more minor capacity. Using a BioID-based proximity-labeling approach, we have identified novel candidate interactors of HOIL1 induced during MDA5 signaling. In addition, we have found that LGP2 is ubiquitinated by HOIL1 during virus infection. Given the central role of LGP2 in …
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Interactions and Functions of the Ubiquitin Specific Protease 7 in Human Cells
… and DHX40 as new USP7 targets. Furthermore, a BioID approach was used to profile the interactions and putative functions of FBXO38, revealing an interaction with KIF20B, a Kinesin-6 protein required for cytokinesis. I show that depletion of either FBXO38 or USP7 result in dramatic decreases in …
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Investigating the role of ULK1 kinase activity during autophagy
… process I performed a proximity ligation assay (BioID) using MRT68921 as a tool to enrich for autophagy regulators trapped on stalled autophagosomes. With this approach ATG2B was identified, among others, as a protein that is enriched on autophagosomal structures upon ULK1 inhibition. I here show …
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Uncovering The Zeb1 Interactome to Identify Novel Regulators of Metastatic Non-Small Cell Lung Cancer
… Here, we apply two independent screens- BioID and an Epigenome shRNA dropout screen- to define ZEB1 interactors that regulate post-translational modification and are critical to metastatic NSCLC. These screens revealed an interaction amongst ZEB1 and the histone deacetylase …
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Characterisation of oncogenic pathways driving the pathogenesis of prostate cancer
… identified as a novel LGMN interactor through a BioID system, which employs promiscuous biotin labelling and subsequent mass spectrometry, and confirmed via co-immunoprecipitation in various models of PCa. Knockdown of LGMN and HSD17B4 is selectively cytotoxic to PCa cells, indicating that …
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