Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"Binding constant"”.
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Biochemical and genetic analysis of nucleic acid-protein interactions
… coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ M$\sp{-1}$ and the nonspecific binding …
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Interaction Between Humic Acid and DNA
… with DNA is determined by the calculation of the binding constant of humic acid with CT-DNA and the quenching phenomenon of humic acid in the presence of DNA is also studied using Stern Volmer Relationship.</p>
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CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE-STRANDED DNA APTAMERS AND THEIR INTERACTIONS WITH PROTEIN TARGETS AND CARBON DOTS
… structures permitting high-affinity binding to target molecules. In the first part of this work, capillary electrophoresis experiments were conducted to determine the dissociation constant of a unique FITC-labeled single-stranded DNA and anti-FITC antibody system in free solution …
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Chemistry and Measurement at Aqueous Nanoscale Interfaces
… morphology, water sorption, longitudinal elastic constant, and thermal transport properties of the film. Although these two areas do not have significant overlap, interfaces play an important role in both systems. When nanoparticles contact biological milieu, proteins adsorb to the surface forming …
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Fundamental and Applied Studies in Asymmetric Catalysis
… L*. 1H NMR measurements revealed that the binding constant of representative primary amines to L* could be tuned through variation of the electronic properties of the substituents covalently bound to boron.
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Characterization of methionine transport in bovine intestinal brush border membrane vesicles
… found to be into an osmotically active space. A binding constant of 75.4 pmol/mg membrane protein was determined. A significant (p<.05) sodium stimulation of methionine uptake was observed. This indicated active (energy-dependent) transport in addition to the diffusive component of intravesicular …
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Interactions of Anticancer Drugs With DNA Studied by High-Resolution NMR Spectroscopy
… minor groove floor, which would explain why the binding constant of WP631 to DNA is 3 orders less than expected. WP631 has also shown tight binding to modified DNA d(CGJACG)$\sb2.$ The 3D structures of these two complexes have pointed the direction for the future design of bisanthracycline drugs.
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An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry
… the enthalpy change associated with a molecular binding interaction. From these data, the binding constant for the reaction can be determined. In the scope of a larger project to design a high sensitivity instrument for collecting such data, the current methods in isothermal titration calorimetry …
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Over-expression and characterization of four alginate lyases from vibrio splendidus 12B01
… 2+ is necessary for optimal enzyme activity. The binding constant (K m ) of the lyases toward alginate was found to be between 22 and 123 mM alginate and the maximum reaction rate (V max ) was found to be between 0.13 and 0.83 μM s -1 . The turnover numbers for the lyases was found to be between …
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A Break In Communication: The Synthesis, Characterization, DNA Binding and Photocleavage of a Novel Ruthenium Polypyridyl Complex Containing an Electronically Isolated Pyrene Group
… experiments including isothermal and competitive binding titrations in addition to the hydrodynamic technique of viscometry were used to show that [Ru(bpy)2bpy-py]2+ binds to DNA with a binding constant of 8 x 105 and 1x106 M-1 (binding constants calculated using isothermal and competitive binding …
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Extracting Equilibrium From Nonequilibrium: Free Energy Calculation From Steered Molecular Dynamics Simulations
… thus obtained, important observables such as the binding constant and conductivity are estimated. And finally, the problem of finding reaction paths is discussed: a new method based on mean first-passage times is proposed and is applied to the excitation migration in photosynthesis.
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Synthesis of Tin Containing Calix[4]arenes and their Anion Binding Capabilities
… may be placed in order to design anion binding hosts of very specific dimensions and selectivities. The purpose of this research is to synthesize a ditopic species that has the ability to simultaneously complex anions and cations starting from the calix[4]arene skeleton. Starting with …
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Interactions of functionalized vesicles in the presence of Europium (III) Chloride
… enthalpy 126 ± 5 kcal/mole and an equilibrium binding constant 26 ± 4 mM-1. The results indicate a molecular complex formed binding two ß-diketone receptors to one Eu3+ ion. Dynamic light scattering (DLS) was used to follow changes in LUV diameter indicated in an increase in vesicle size …
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The synthesis and investigation of the electronic properties of crown ether, [2]-catenane, and [2]-rotaxane architectures
… rotaxane, and catenane genres. The binding constants of thiophene and phenylene-ethynylene based crown ethers are determined via fluorescence quenching titrations, in an attempt to correlate structure with binding affinities. The insight obtained from the binding constant …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… focuses on the measurement of the intracellular binding constant between focal adhesion proteins of interest, GFP-Paxillin and FAT-mCherry, using two-photon excitation fluorescence microscopy and the utility of it as a measure of protein conformational change. The hypothesis tested is that …
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Assembly and detection of viruses and biological molecules on inorganic surfaces
… and consensus sequences were achieved. Virus binding was characterized using Atomic Force Microscopy, Fluorescene Microscopy and Titration. Using a simple bimolecular model, the binding sequence identified through this work is shown to have a binding constant 100,000 times better than a random …
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Extracting Equilibrium From Nonequilibrium: Free Energy Calculation From Steered Molecular Dynamics Simulations
… have already proved to be effective and are constantly being improved. Fluctuations play such an important role at the nanometer scale where biomolecules live that the theory of fluctuations (commonly known as nonequilibrium statistical mechanics) is indispensable for understanding the …
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Catalytic Reduction of NO and N2O with Hydrogen in Water by Polymer-Protected Palladium Nanoparticles
… This is consistent with expectations that the binding constant of NO to the palladium nanoparticle surface is considerably larger than that of N2O. Molar balances and post-experimental ammonia analysis confirmed that no side production of ammonia was occurring. Further studies showed that no …
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Enhanced polymeric nanoparticles for gene delivery
… a high-throughput assay to quantify polymer/DNA binding as a gene delivery bottleneck and find that a biphasic relationship exists between polymer/DNA binding constant, Ka, and delivery efficacy. We also show that end-modified PBAEs can be as effective as adenovirus for gene delivery. In …
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Fluorescence photophysics, characterisation and application of labelled glucose/galactose binding protein
… glucose sensor is bacterially-derived glucose binding protein (GBP). In this project, the modified triple mutant H152C/A213R/L238S-GBP (mGBP) was investigated for viability as a functioning glucose sensor. Labelling the protein with the environmentally-sensitive dye BADAN produced repeatable, …
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