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Showing 1 to 20 of 145 for “"Binding Studies"”.
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Topologically constrained DNA intercalators: Synthesis and DNA binding studies
The synthesis and DNA binding properties of three macrocyclic diacridines (31, 32 and 33) is discussed. The two acridines are connected by linker chains at the 4- and 9-positions. Macrocycles 31 and 32 are linked via spermine at the 9-position and have been shown to bind to DNA by bisintercalation …
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Synthesis and DNA-binding studies with two stericallyfriendly porphyrin frameworks
… porphyrins play a significant role during binding with DNA hosts. Possible applications of these systems in photodynamic therapy as well as in anti-bacterial and anti-cancer therapies motivate the binding studies. For characterizing DNA binding motifs different methods are useful including …
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Human Serum Albumin Characterisation and Binding Studies by Spectroscopic Techniques
… of characteristic "marker ligands," influences binding to provide a handle that can be utilised and monitored. This enables the assignment of binding sites and the study of perturbating conditions.<br/>Changing conditions such as pH, temperature, ionic strength and solvents in the presence and …
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Development of a TIRF Microscope for Single Molecule Binding Studies
… signals as outliers, and no detection of binding. SMI was established with a compatible 488 nm-50 mW laser for Alexa Fluor 488, but not for Cy3 that would require another appropriate laser. During the development of our TIRFM, the problems that we encountered and addressed are discussed, …
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Structural and Inhibitor Binding Studies of Tyrosyl-DNA Phosphodiesterase 1
… and application of fluorescencebased binding assays to study TDP1-ligand binding interactions. Two fluorescence-based binding assays were investigated. These include thermal shift assay and intrinsic protein fluorescence spectroscopy. The results obtained by thermal shift assay were …
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Oligonucleotide Binding Studies of Transfer Ribonucleic Acid Structure: Crystal and Solution
Made available in DSpace on 2014-12-14T04:27:42Z (GMT). No. of bitstreams: 1 7804053.pdf: 9334542 bytes, checksum: 97fb936a1b4500ec076253a65926f736 (MD5) Previous issue date: 1977
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Single Molecule Ligand Binding Studies on CCR5 by Fluorescence Cross-Correlation Spectroscopy
… inhibition constants for the RANTES analogues binding to CCR5. We developed a tandem affinity purification protocol to purify full-length, monomeric CCR5 from receptor truncations and oligomers. We fluorescently labeled CCR5 using the SNAP tag, a functional tag derived from DNA alkyl …
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Secretomotor substances in the rat lacrimal gland: Immunocytochemical functional and ligand binding studies
… nonadrenergic nervous pathway. Recent studies have demonstrated the presence of peptidergic nerves, and the neuropeptide VIP is established as a lacrimal secretagogue, however the involvement ofother neurotransmitters in the control of lacrimal function remains unclear. Ageing has been …
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Structural and ligand binding studies of toll-like receptor 4 (TLR4) associated proteins
… hydrophobic patch, which may facilitate LPS binding. In Chapter 3 we describe the expression and purification of mouse CD14 as an additional target for ligand binding crystallization studies. After determining that purified human and mouse CD14 bind LPS and other ligands using native PAGE gel …
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Development and application of quantitative ligand-binding studies to elucidate mechanistic aspects of pentameric ligand-gated ion channels
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
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Designing Novel Blue Copper and Purple CuA Centers in Azurin With Natural and Unnatural Amino Acids
… was used to incorporate a 2Fe-2S cluster binding loop from human ferrochelatase into azurin. Preliminary iron binding studies indicate the assembly of a 4Fe-4S or a 2Fe-2S iron sulfur cluster.
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Design and synthesis of substituted cyclopropanes as conformationally restrained dipeptide mimics
… activity is the most important aspect of studies of molecular recognition. Enzyme binding studies are complicated because of the numerous variables in the binding process, which include the flexibility of substrates and inhibitors in solution, the paucity of enzyme structural information, …
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Binding, Thermodynamic and Structural Studies of High-Affinity T Cell Receptor-Peptide MHC Interactions
… spectroscopy was used in stability and binding studies of scTCRs. Urea denaturation analysis was used to show that scTCRs engineered in the yeast display system have similar stabilities as single chain antibody Fvs. The yeast display system was successful in determining a location for …
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