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Showing 1 to 20 of 27 for “"Biacore"”.
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Antibody-based biosensor assays for the detection of zilpaterol and markers for prostate cancer
… in a competitive ELISA format and on the Biacore (a surface plasmon resonance-based optical biosensor capable of monitoring biomolecular interactions in 'real-time'). A zilpaterol-HSA conjugate was used to generate and characterise single chain antibody fragments. A combinatorial single …
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Signalbindung und Membraninteraktion von heterotetrameren Adaptorprotein-Komplexen
… mittels Oberflächenplasmonresonanz-Analyse (Biacore) in vitro untersucht. Alle getesteten Signale interagierten kaum mit AP4, aber gut mit AP3. Die Bindung an AP1 und AP2 war deutlich heterogener. Am auffälligsten dabei war, dass die lysosomalen Proteine LAMP-1 und LAMP-2B gut an AP1 banden, …
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Novel anitbody-based assays for disease and food contaminant detection
… and subsequently used in an indirect ELISA and Biacore assay for the detection of antibodies to C. pseudotliberculosis in sheep serum samples. These assays were optimised using a variety of positive and negative controls and were then applied to a range of clinical samples from Irish herds. A …
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Generation and application of recombinant antibody fragments for prostate cancer detection.
… kinetic analysis of candidate antibodies by Biacore. A differential expression profile of an anti-fPSA scFv and reformatted scAb was performed by ELISA. Individual antibodies isolated from the ‗biopanning‘ process were then characterised. The murine anti-fPSA antibodies were further analysed …
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Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems
… available sensor chips. We have shown that BIAcore(TM) SA chips maintain their surface binding functionality after pressure exposure and that effects to the surface are reversible to 6000 psi. Three test systems were chosen to be studied using this instrument, each having different …
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TARGETING A CANCER-ASSOCIATED BIOMARKER WITH DIAGNOSTIC AND THERAPEUTIC RADIOACTIVITY
… IL13Ra1/IL4Ra in cells nor binds to it in Biacore studies. Furthermore, PET imaging confirmed in vivo targeting of systemically delivered 124I-TQM13 to IL13Ra2-expressing subcutaneous GBM tumors and 131I-TQM13 demonstrated therapeutic efficacy in such animal model. Our results demonstrated …
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting Clostridium difficile enterotoxin A
… binding activity against TcdA was assessed via Biacore. Our studies also led to medium simplification for small-scale expression, shorter expression time and simpler purification process without impairing on the quality of the expressed recombinant protein.
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Genetic engineering of antibody fragments for the detection of illicit drugs
… of illicit drugs and their application in ELISA, Biacore surface plasmon resonance -based biosensor assays and, finally, in a multidrug-based assay. The first phase of this work focused on the development of a repertoire of antibody fragments against various illicit drugs including …
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Understanding the Molecular Level Interactions of Cancer Inhibitor Imatinib with Human Fibroblast Growth Factor-1
… and solvent accessibility. In addition, biacore analysis was carried out to determine the binding affinity of imatinib to hFGF-1.</p>
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Quantification of the Binding of Insulin-like Growth Factor-I (IGF-I) and IGF Binding Protein-3 (IGFBP-3) Using Surface Plasmon Resonance
… carboxymethylated dextran (CMD) surface, a BIAcore 2000 with a high molecular weight CMD surface and a Leica SPR 2001 Alpha with a planar mixed self-assembled monolayer (mSAM) surface. Since the experimental system we used was transport sensitive, only the mSAM surface, under optimized …
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Functionalised metal nanoparticles as novel reagents for biomedical analysis.
… biorecognition systems was investigated by the Biacore Surface Plasmon Resonance technique and this work is described in Chapter 7. Finally, a summary of conclusions and some suggestions for future work are presented in Chapter 8.
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Integrin-ligand interactions and cytokine production by monocytic cells
… purified αVβ3 and αVβ5 integrins in in vitro Biacore assays. These peptides all contained a common basic tripeptide motif, termed the Arg-Lys-Cys (RKC) motif. Integrins traditionally recognise and bind the Arg-Gly-Asp (RGD) tripeptide in their ligands in a cation-dependent manner. However, the …
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Towards an antibody mediated resistance against Geminiviruses
… scFvs were determined by western blot, ELISA and BIACORE. The missing nucleotide found in the hybridoma derived scFv-SAV against AV1 was restored and the resulting scFv (scFv-RW-AV) showed better binding properties than scFv-SAV. Four scFvs against AV1 were derived from the naïve Tomlinson I …
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Klonierung, Expression und Charakterisierung von Mutanten des Bone Morphogenetic Protein-2
… der Mutanten zu Rezeptor und Matrix in Biacore-Messungen konnte die Änderung des Wirkungsgrades auch in einem Zellkulturassay mit einer Maus-Fibroblasten-Zellinie durch Messung der Alkalischen Phosphatase und im Hühnergliedmaßentest gezeigt werden. In in vivo Experimenten bleibt eine …
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Structure and function of potassium and calcium channels
… were carried out using GST fusion proteins, Biacore surface plasma resonance and FRET investigations. These confirmed that the N and C- terminal domains are important in determining activation kinetics, and that these regions interact. To determine the positions of both the N- and C- terminal …
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von Willebrand factor interaction with subendothelial collagens and platelet surface receptor GPIBalpha under shear conditions
… characterized using Surface Plasmon Resonance (BIAcore). Compared to binding to pepsin-digested collagen III surfaces, normal vWf multimers (nvWf) bound to human pepsin-digested collagen I with a 5-fold lower binding capacity and a 10-fold higher equilibrium dissociation constant. There was no …
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Molecular and Genomic Based Insights Into The Evolution of Enterococcus Faecium From Commensal to Hospital-Adapted Pathogen
… they are ubiquitous in both clades). ELISAs and BIAcore analyses also showed that proteins encoded by these operons bind several different host extracellular matrix proteins, as well as to each other, suggesting a novel cell-surface complex. In summary, my studies provide new insights into the …
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Monoclonal antibody (mAb) purification by counter current chromatography (CCC)
… to Protein A was seen, as confirmed by Protein A BiaCore analysis. CCC machines were seen to adversely affect IgG functionality. This led to a systematic investigation of the effect of CCC phase mixing on IgG functionality in a number of different CCC instruments, allowing direct comparisons of …
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Generation and characterisation of plant produced recombinant antibodies specific to LHRH for treatment of sex hormone dependent diseases
… parental murine mAb, as measured by SPR using BIAcore, were found to be indistinguishable. Incubation of chimeric rAb and the mouse mAb in human serum in vitro revealed high stability of both the mouse mAb and the chimeric antibody at the physiological temperature. Antibody activity after 32 …
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