Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Beta-glucuronidase"”.
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The Determination of Glucuronides in Biological Fluids by Lucigenin Chemiluminescence (beta-Glucuronidase, Glucuronic Acid, Immobilized Enzyme, (Hplc))
The use of certain chemiluminescence reactions as detection techniques for organic compounds has been explored during the past decade. In this research project the chemiluminescence reaction between alkaline lucigenin (10,10'-dimethyl-9,9'-biacridinium nitrate) and glucuronic acid (an important …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
… by use of excess levels of a second enzyme, (beta)-glucuronidase to remove all nonreducing terminal D-glucuronic acid residues from oligosaccharides present in the reaction mixture. This action prevented the oligosaccharides from participating in the transglycosylation reaction. Conditions …
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Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function
… phosphatase, acid phosphatase, lysozyme, $\beta$-glucuronidase, and myeloperoxidase was quantitated and was not affected by treatment.
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Analysis of organ-specific gene expression in soybean
… of a foreign gene. The reporter gene $\beta$-glucuronidase (GUS), was linked to the 5$\sp\prime$ and 3$\sp\prime$ sequences of the lectin gene and transferred into Arabidopsis thaliana and Nicotiana tabacum. GUS activity assayed on transformed tissues revealed seed-specific expression of …
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Toxicology and herb-drug interaction of selected anti-hypertension plants used by lay persons in Northern KwaZulu-Natal (South Africa)
… applied were the CYP3A4 inhibition assay, the beta-glucuronidase inhibition assay as well as the carboxylesterase inhibition assay. These assays were chosen according to the enzymes involved in the metabolism of the hypertension conventional drugs. The overall toxicity profile of the plant …
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One- and Two -Photon States for Quantum Information
… expression level based on the activity of beta-glucuronidase of 158 +/- 9 units/mg protein (mean +/- SEM; n=3; variance within SC lines are expressed as standard error of the mean SEM) indicating a significantly higher level of expression, as compared to MC lines that had a much lower …
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Understanding polyamine metabolism through transgenic manipulation in poplar suspension cultures
… control cell line that was transformed with the beta-glucuronidase (GUS) gene.</p><p>It was observed that enhanced putrescine metabolism resulted in: (i) increased expression of arginine decarboxylase gene, along with enhanced activity of the corresponding enzyme, (ii) decreased expression of …
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An Interdisciplinary Approach: Computational Sequence Motif Search and Prediction of Protein Function with Experimental Validation
… through gene gun bombardment assays using the beta-glucuronidase reporter system, which revealed that there is a high likelihood that the candidate can enter the soybean cells.
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Complementation of the salt sensitive S. cerevisiae mutant cch1 with an A. thaliana cDNA library and analysis of isolated genes
… cDNA clones were fused to the luciferase and the beta-glucuronidase gene and integrated into the Arabidopsis genome. The obtained results indicate that the isolated genes are involved in a more general response to abiotic stresses since inductions after salt, cold and ABA treatments could be …
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Calcium -dependent protein kinases are myristoylated and associated with different membranes in Arabidopsis thaliana
… binding. Using a translational fusion with the beta-glucuronidase (GUS) reporter gene, I have demonstrated that the amino terminal region of AtCPK2 and AtCPK5 contains sufficient information for correct membrane localization.</p><p>My results indicate that myristoylation is important in membrane …
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Genetic and Biochemical Characterization of Methanol Utilization in Methanosarcina Acetivorans C2A: Analysis of the Methanol:methyl-Coenzyme M Methyltransferase System Encoded by mtaCB and mtaA
… to methanol utilization, the gene uidA encoding beta-glucuronidase from E. coli was demonstrated as a suitable reporter gene for Methanosarcina . Additionally, the E. coli proC gene was developed as a positive selectable marker further increasing the capacity to use genetics in the analysis of …
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Identification and Characterization of a Calmodulin -Binding Protein Related to a Family of ATPases Involved in Cell Division and Membrane Fusion
… expression of the CIP111 promoter fused to a beta-glucuronidase reporter gene in transgenic plants demonstrated that CIP111 is expressed in young anthers, developing embryos, root tips, and lateral roots. An unusual characteristic of higher plants is that they express a variety of CaM …
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Soybean Mosaic Virus Hc-Pro Enhances Viral Symptoms, Bean Pod Mottle Virus and Transgene RNA Stability, siRNA Accumulation, and Reduces Soybean Seed Production
… plants partially stabilized the expression of a beta-glucuronidase (GUS) transgene that was susceptible to PTGS. SMV infection stabilized GUS gene expression, enzyme activity, and enhanced GUS siRNA accumulation.
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Evaluation of translation initiation features of satellite tobacco necrosis virus RNA
… initiation. Translational studies of $\beta$-glucuronidase transcripts with their 5$\sp\prime$-untranslated leaders replaced with the 5$\sp\prime$-untranslated leader of STNV RNA, modified to eliminate possible hybridization with 18S rRNA, demonstrate that the 18S rRNA complementary site …
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
… between the calmodulin-3 promoter and the $\beta$-glucuronidase reporter gene were introduced into tobacco plants to the biological roles of the protein binding sites identified in vitro. Consistent with activity of similar sequence elements in the cauliflower mosaic virus 35S promoter, the …
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Taraxacum officinale as an expression system for recombinant proteins : molecular cloning and functional analysis of the genes encoding the major latex proteins
… containing the ppo promoter region fused to the beta-glucuronidase (GUS) gene. GUS activity was restricted to the laticifers. Expression level was 50–fold higher in the laticifers than the expression level conferred by the CaMV 35S promoter in the same tissue. A number of potential cis-regulatory …
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Effects of diet and loss of ovarian hormone production on adiposity, inflammation, cecal integrity, and gut microbial communities of rodent models
… of aim 4 was to determine the bacterial beta-glucuronidase activity level and the longitudinal shifts of the gut microbiota following the loss of ovarian function and progression of obesity in mice fed a HFD. Fecal pellets were collected at baseline (wk 0, prior to ovariectomy surgery but …
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Characterization of a potato Proteinase Inhibitor II gene whose expression in not wound-responsive
… (chloramphenicol acetyl transferase, CAT; or [beta]-glucuronidase, GUS) coding sequences in constructions that contained the terminator from the wound-inducible pin2K gene. The chimeric genes were transferred to tobacco plants using Agrobacterium tumefaciens. The presence of the constructions …
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Expression of atrial natriuretic factor in transgenic potatoes
… or tuber-specific expression of cytosolic beta-glucuronidase (GUS) to confirm the functionality of the DNA regulatory sequences used. All six chimaeric gene fusions were cloned into the binary vector, pGA643, or its derivative. These were then introduced into potato plants via leaf disc …
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THE DOUBLE BOND IN Δ1-2 POSITION OF STEROIDS: A MATTER OF CONTROVERSY IN THE CONTROL OF ILLICIT TREATMENTS OF FARM ANIMALS
… Androstenedione, androstadienedione, alfa and beta boldenone, testosterone and epitestosterone transformations were investigated. Mutual interconversions of steroids were observed, although 17 beta hydroxy steroids had lower stability if compared with 17 alfa hydroxy and 17-keto steroids. This …
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