Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"BeWo"”.
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The Impact of Excess Nutrients, Fatty Acids or Glucose, on BeWo Placental Trophoblast Metabolic Function
… system. The impacts of nutrient overabundance on BeWo trophoblasts (an in vitro model of both progenitor cytotrophoblasts and differentiated syncytiotrophoblasts) were specifically examined using functional readouts of cellular metabolism and mitochondrial respiration, measurements of nutrient …
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The Role of MicroRNA-378a-5p and its Target Genes in Differentiation and Fusion of BeWo Cells
… by using a choriocarcinoma cell line, BeWo. Using luciferase reporter assays, we showed that miR-378a-5p targets CREB. Transfection of miR-378a-5p decreased, while antimiR-378a-5p increased CREB protein and mRNA levels. We found that a miR-378a-5p target gene, Nodal, induced STB …
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Ryanodine receptor expression in trophoblasts
… are expressed in model trophoblast cell lines (BeWo and JEG-3) and in human first trimester and term placental villi. Immunohistochemistry of human placental sections indicated that both syncytiotrophoblast and cytotrophoblast cell layers were positively stained by antibodies recognising RyRs; …
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Caractérisation du mécanisme d'action du récepteur sérotoninergique de type 5-HT2A dans les cellules de choriocarcinomes placentaires humains BeWo et JEG-3
… de choriocarcinomes placentaires humains, BeWo et JEG-3. (Abstract shortened by UMI.)
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The role of megalin in the transport of aminoglycosides across human placenta
… q-PCR (2) The human choriocarcinoma cell line (BeWo cells) were grown on Transwell plates, and then megalin expression and function were assessed. Results: Megalin protein and mRNA expression were confirmed in samples of human placental villous tissues. Megalin mRNA expression declined steeply …
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ROLES OF SULFOTRANSFERASE ENZYMES IN TRANS-PLACENTAL DISPOSITION
… cytotrophoblast cells and the trophoblast-like BeWo cell line. These isoforms have been reported to be functional in human placenta. Our results indicated that the phenolic sulfotransferase isoforms, SULT1A1 and SULT1A3, are functional in BeWo, as well as in the primary cytotrophoblast cells. …
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Regulation and functional significance of ATP binding cassette transporters in human placenta
… human placenta, cultured primary trophoblast and BeWo and Jar trophoblast-like cell lines. Jar cells were found to be more similar to nondifferentiated cytotrophoblast with respect to their ABC protein expression profile, whereas BeWo cells more closely reflected differentiated …
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Poly (ADP-ribose) polymerase-1 (PARP-1) induces human trophoblast syncytialization
… Lastly, human PARP-1 was overexpressed in the BeWo trophoblast derived cell line, under fusion promoting conditions, to directly test the ability of PARP-1 to regulate trophoblast fusion. Throughout the first and second trimester, PARP-1 and Snail were highly expressed and co-localized in …
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Maternal stress signature and infant neurodevelopment: determining the beneficial effects of human milk oligosaccharides - an in vitro study
… placental barrier using transwell inserts and BeWo cells. BeWo cells, a choriocarcinoma cell line replicating trophoblasts, were chosen as an in vitro placental barrier model as they are capable of replicating two important functions of the placenta - trophoblast fusion and transplacental …
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Determination of nicotine and its metabolites by capillary electrophoresis and mass spectrometry.
… in-vitro human trophoblast-like cell line, BeWo, via HPLC/MS and CE/UV. Analysis of urine samples was accompanied by sample clean up via SPE to ensure the appropriate removal of inorganic salts. An optimised hydrodynamic and electrokinetic injection method (HE injection) was used for …
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Deep vertebrate roots for mammalian krab zinc-finger transcription factors
… and predicted targets for the protein in human BeWo choriocarcinoma cells. Genes neighboring ZNF777 binding sites can be either up- or down- regulated, suggesting a complex regulatory role. Our studies revealed that some of this complexity is due to the generation of HUB-containing and HUB-minus …
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Folate Receptor-Targeted Transplacental Delivery of Digoxin for the Treatment of Fetal Arrhythmia
… release characteristics were determined. Using BeWo (choriocarcinoma) cells as an in vitro transplacental transport model, we were able to show that these nanoparticles increase permeability of digoxin across the placenta by evading placental efflux. Conjugation of folic acid to the surface of …
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Placental co-culture model reveals Listeria monocytogenes utilization of monocytes for vertical transmission
… A novel cell co-culture system comprised of BeWo and HPVEC cell lines was established to model the human placental barrier. Barrier integrity was confirmed using immunostaining of tight junctions and a FITC-dextran permeability assay. The model was then used to observe L. monocytogenes use of …
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The detection and role of human endogenous retroviruses in multiple sclerosis and other neurological diseases
… high levels of transcription occurring in the BeWo choriocarcinoma cell line. In the astrocytoma cell lines, those with P53 mutation had higher levels of HERV-W env. MSRV gag variants were also detected in these cell lines, but stimulation with interferon-γ, a proinflammatory cytokine, did not …
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Markers and mechanisms of pre-eclampsia in pregnancies complicated by diabetes
… supernatants were determined by ELISA. JAR and BeWo cells were cultured on semi-permeable membrane inserts in Transwell plates for up to 16 days, then exposed to N-LDL vs. HOG-LDL. Transepithelial electrical resistance (TEER) was measured after 6, 12 and 24 hours of treatment and …
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Paclitaxel nanoparticles for breast cancer therapy in pregnancy
Breast cancer can complicate pregnancy and result in fetal and maternal morbidity and mortality. These effects are often compounded by restrictions in the diagnosis and treatment of breast cancer due to implications on fetal health. A number of new paclitaxel nanoformulations for the treatment of …