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Showing 1 to 14 of 14 for “"Bc1 complex"”.
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Protein Coevolution and Coadaptation in the Vertebrate bc1 Complex
The cytochrome bc1 complex of the mitochondrial electron transport chain accomplishes the enzymatic reaction known as the modified Q-cycle. In the Q-cycle the bc1 complex transports protons from the matrix to the intermembrane space of the mitochondria, creating the proton gradient used to make …
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Theoretical study of the cytochrome bc1 complex reaction mechanism
The bc1 complex is an enzyme which plays a critical role in energy production through photosynthesis and cellular respiration. Its biochemical function relies on the so-called Q- cycle, which is well established and operates via quinol substrates that bind the protein at their active sites. Despite …
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The Structure-Function Interface in the Cytochrome Bc1 Complex Family
Mitochondrial bc1 complexes and chloroplast b6f complexes belong to a common family of quinol oxidizing enzymes. We have made a systematic survey of the redox subunits of the bc-type quinol oxidases for all known bacterial sequences and have identified six new representatives of the enzyme. …
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Biochemically realistic MD and kinetic models of the Rhodobacter sphaeroides bc1 complex
… for a more natural model of the Rb. sphaeroides bc1 complex which in past studies has lacked several details in composition of the fatty acids and relative quantities of each lipid. Past studies have shown some distortion on MD relaxation relating to a large void volume in the protein structures. …
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Heterogeneity of the Bc1 Complex Subunits and Qo Site Occupants From Rhodobacter Sphaeroides
… do not crystallize easily. Trials to crystallize bc1 complex from R. sphaeroides, a membrane-bound four-subunit respiratory protein that shares physical and catalytic properties with the vertebrate bc1 complex, have been undertaken and results help understand the problems of heterogeneity and …
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Identifying the mechanism of second proton release from the bc1 complex Qo-site
The cytochrome bc1 complex and its family members play a central role in biological energy transduction across all domains of life, oxidizing QH2 in a bifurcated reaction which generates a proton-motive force used for ATP synthesis. It is widely accepted to operate according to a modified Q-cycle, …
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Qo site of the bc1 complex; unlocking the gate of the electron transport chain
The bc1 complex of the electron transport chain is critical to production of ATP through the proton gradient driven by quinone oxidation; a ubiquitous mechanism for energy conversion throughout the biosphere. In addition to being a central source of energy transduction, its bypass reactions can …
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Spectroscopic and Electrochemical Analysis of the Rieske Iron-Sulfur Protein of the Rhodobacter Sphaeroides Bc1 Complex
… subunit of Rhodobacter sphaeroides cytochrome bc1 complex was isolated and then investigated using molecular spectroscopies (high-resolution electron paramagnetic resonance and resonance Raman), protein-film voltammetry, and x-ray crystallography. Major accomplishments include the following: …
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Exploring the Quinol Oxidation Mechanism at the Qo-Site of Bc1 Complex Through Site-Directed Mutagenesis
The backbone amide groups of FL132 and FC134 give additional electron withdrawing force to the Rieske-type [2Fe-2S] cluster and the residues in the 132-134 positions play roles in anchoring the ISP to the surface of the Qo-pocket. FG133 has unique ϕ/psi angles that restrict the positions of …
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Electron gating and mechanism of proton exit in quinol oxidation in cyt bc1 complex from R. sphaeroides
The cytochrome bc1 complex of Rhodobacter sphaeroides in wild-type and several strains mutated at the conserved -PEWY- glutamate residue (E272 in chicken, E295 in R. sphaeoroides) were characterized in situ to determine the functional role of this residue in the second electron transfer of quinol …
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Characterization of alternative enzymes in bacterial respiration
… prokaryotes to the multicellular and highly complex eukaryotes and they largely derive this as a byproduct of respiration. Bacteria are known for their branched and highly modular aerobic and anaerobic respiratory chains. The branching refers to the presence of multiple respiratory enzymes …
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Ligand Binding and Structural Dynamics in C-Type Cytochromes
… of the interaction of cyt c and yeast bc1 complex are described. Contrary to results from crystallographic studies, the simulations reveal multiple dynamic hydrogen bonds and salt bridges in the cyt c-c 1 interface. A novel structural change in cyt c is also reported, involving residues …
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Steered molecular dynamics: a tool to investigate molecular interactions
… of the domains of the mitochondrial cytochrome bc1 complex controls the bifurcation of the electron transfer governing the energy transduction in the complex. A solvated structure of the complex in a phospholipid bilayer was modeled. The domain movement was induced in SMD simulations by applying …
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Interactions of Fe(II) with the iron oxidizing bacterium Rhodopseudomonas palustris TIE-1
… center, enter the quinone pool through the bc1 complex. This model is significantly different from previous preferred models of phototrophic oxidation, but is similar to the reverse electron transfer system described in acidophilic lithotrophic iron oxidizing bacteria. Taken together, the …