Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Basal cells"”.
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Sequence-Specific Gene Correction of Cystic Fibrosis Airway Basal Cells
… with an efficiency of ~10 % in CF airway basal cells homozygous for the ΔF508 mutation. Basal cells are a multipotent stem cell population of the respiratory epithelium and therefore, their gene correction could provide a long-term, permanent remedy for CF. Delivery of engineered …
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Characterization of Stem Cell Turnover In A Living Epithelial Bilayer
… the renewal and replacement of old or dying cells while sustaining a functional barrier. Imbalance between cell production and elimination are hypothesized to underlie many pathological conditions. However, our knowledge of cell turnover within living tissues remains largely restricted to …
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Investigating The Role of The P63 Isoform Δnp63 In Lung Stem Cell Populations and Lung Cancer
… separate populations of lung progenitor cells that give rise to lung adenocarcinoma and squamous cell carcinoma. ΔNp63 is known to regulate lung development but its role in lung cancer progression remains unclear. We utilized a ΔNp63-specific conditional knockout mouse model to determine …
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Stem and Progenitor Cells in Homeostatic Maintenance and Perturbation of the Pulmonary Epithelium
… of mutated tissue stem or progenitor cells to this process are poorly understood at the level of clonal resolution. Secretory cells are known progenitor cells of the pseudostratified airway epithelium, however their clonal dynamics in homeostasis are unknown. Here, I elucidate their …
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The Role of Hydrocortisone on the Development and Maintenance of Ion Transport Pathways Essential to Mucociliary Clearance in Differentiated Normal Human Bronchial Epithelial Cells
… for recruitment and activation of immune cells. Chronic inflammation of the airways due to prolonged or repeated exposure to noxious agents can result in epithelial remodeling and fibrosis that leaves the airways more susceptible to infection due to disruption of innate immune processes. …
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Specification of Stem Cells and Niche During Hair Follicle Morphogenesis
… live imaging, I found that in developing HFs, basal cell divisions are asymmetric and perpendicular to the basement membrane. These divisions result in differential levels of WNT signaling in the daughter cells, with basal cells remaining WNT<sup>high</sup>, and suprabasal cells becoming …
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Changes in buccal cytome biomarkers in relation to ageing and Alzheimer’s Disease.
… to investigate the possibility of using buccal cells derived from a multi layered epithelial tissue from the oral mucosa as a model to identify potential biomarkers of genomic instability in relation to normal ageing and premature ageing syndromes such as AD and DS. A buccal micronucleus cytome …
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Decoding Breast Cancer Development Through Single Cell and Spatial Genomics
… is a preinvasive breast cancer in which cancer cells remain confined within the lumen of ducts. With the adoption of mammography, DCIS diagnoses have risen significantly, with approximately 50,000 new cases each year in the U.S. While 10-30% of DCIS patients progress to invasive disease (IDC) …
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Regulation of Asymmetric Cell Divisions in the Developing Epidermis
… the many levels that regulate ACDs. Within the basal layer of the epidermis, both symmetric and asymmetric cell divisions occur. While symmetric divisions allow for an increase in surface area and progenitor cell number, asymmetric divisions drive the stratification of the epidermis, directly …
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Human Salivary Gland Stem/Progenitor Cells for Tissue Engineering, SARS-CoV-2 Susceptibility, and Neurointegration
… derived salivary gland stem progenitor cells (hS/PCs) along with those isolated from major glands to understand salivary gland susceptibility to SARS-CoV-2, while also contributing data on mechanisms that might explain the ability of coronaviruses to infect salivary glands in the absence …
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Sox2-Dependent Transcriptional Control of Airway Differentiation In The Mouse Lung
… airways contain four lung cell types: club cells, ciliated cells, basal cells, and neuroendocrine cells.SOX2 is a well-known conducting airway marker. SOX2 is a transcription factor that is known to be important in embryonic development and induction of pluripotent stem cells. We …
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Coordinating Stem Cell Behavior in the Hair Follicle
<p>Tissue stem cells perform important functions throughout an organism's life. They generate new cells to replenish cells that are lost during normal wear and tear or in response to acute injury. Remarkably, hair follicles naturally undergo repetitive cycles of regeneration and degeneration, a …
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Hot particle dosimetry using a wall-less tissue equivalent proportional counter
… to the target tissues at risk such as epithelial cells in lung and gut and basal cells in skin difficult. Furthermore, current methods fail to determine changes that might occur in radiation quality as the charged particles lose energy and their stopping power increases with depth of penetration …
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In vitro model of periodontal epithelium
… porcine periodontal ligament epithelial cells (PLE cells) were cultured from the epithelial rests of Malassez. The obtained epithelial cell lines were grown on a porous membrane that allows a good cell adhesion and access of the nutrients from both basal and apical directions, and which …
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Investigating factors governing cell fate decisions in respiratory epithelium
… of airway epithelial progenitors/stem cells into mature multiciliated cells (MCCs). Here, I have identified 2 novel transcription factors, Fank1 and Jazf1 which form part of the transcription factor cascade regulating multiciliogenesis in adult and embryonic mouse tracheas. Mouse …
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Role of pericytes in the barrier task
… of dense capillary system of marginal epithelial cells with surrounding pericyte cells (PCs) and a basement membrane of mesodermal basal cells, interconnected by tight junctions. In both barriers the PCs have a crucial role: their functions are linked to regulating cerebral blood flow, barrier …
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Mechanisms of human papillomavirus and host gene transcriptional deregulation in cervical carcinogenesis
… of HPV16 oncogenes E6 and E7 in proliferating basal cells of the cervical squamous epithelium. The major risk factor associated with cervical neoplastic progression is integration of HRHPV into the host genome, which is detected in $~$85% of HPV16-positive cervical carcinomas. The work …
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Expression and regulation of N-Myc Downstream- Regulated gene 1 in squamous cell carcinoma of the oesphagus
… to track with epithelial cell maturation where basal cells of normal oesophageal epithelium displayed plasma membrane-associated NDRG1 while maturing cells were mostly positive for NDRG1 in the cytoplasm and nucleus. Likewise, NDRG1 displayed interesting patterns of localisation in tumour tissue …
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Immortalisation, characterisation and differentiation of temperature sensitive cell lines from the Olfactory Neuroepithelium
… is of unique interest since, unlike other neural cells, olfactory neurons are continually replaced - a feature that is dictated by their direct exposure to the damaging external environment. Basal cells in the olfactory placode are the source of this replacement. Each olfactory neuron expresses …
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The Role of Spindle Orientation in Epidermal Development and Homeostasis
… mitotic spindle orientation in the proliferative basal cells is crucial not only for dictating daughter cell fate but also for initiating stratification of the entire tissue. A conserved protein complex, including LGN, Nuclear mitotic apparatus (NuMA) and dynein/dynactin, plays a key role in …
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