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Showing 1 to 18 of 18 for “"Barkeri"”.
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Analysis of Methylotrophic Methanogenesis in Methanosarcina Barkeri Fusaro
Methanogens are anaerobic microorganisms that are able to gain all of their energy for growth by converting a limited number of substrates to methane gas. Methylotrophic methanogenesis involves the disproportionation of methylated compounds, such as methanol, to methane and carbon dioxide. In this …
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Analysis of hydrogen metabolism in methanosarcina barkeri fusaro
Made available in DSpace on 2010-05-14T20:51:15Z (GMT). No. of bitstreams: 3 Kulkarni_Gargi.pdf: 26285517 bytes, checksum: 09a83a385fb35766510a0bf93e3d07d8 (MD5) 1_Kulkarni_Gargi.pdf: 26264601 bytes, checksum: bedca9c1fe77d00d0cde15df38d30446 (MD5) license.txt: 4064 bytes, checksum: …
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Kristallstruktur der Selen-abhängigen Nicotinat-Dehydrogenase aus Eubacterium barkeri
Eubacterium barkeri ist ein gram-positives, anaerobes Bakterium, das zur Ordnung der Clostridien gehört. Es ist der einzige bekannte Organismus, der Nicotinat fermentativ zu Propionat, Acetat, CO2 und NH4+ abbauen kann. Initiert wird dieser Abbauweg durch eine Selen-abhängige …
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An updated genome scale metabolic reconstruction of methanosarcina barkeri
Methanosarcina barkeri is a methanogen, meaning that methane is the reduced product for energy generation. M. barkeri can grow on several substrates including methanol, acetate, methyl amines and a combination of hydrogen and carbon dioxide. The Methanosarcina barkeri genome was published in 2006 …
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Hydrogenase utilization and regulation in species of methanosarcina
… was previously demonstrated for Methanosarcina barkeri. Both uses of H2 require the activity of three different hydrogenase enzymes, however, M. barkeri can also utilize an electron transport system that is independent of hydrogenase activity. In Chapter 2, the interconnected nature of …
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Electron Transport in Methanosarcina: Pathway Heterogeneity Within the Genus
… in the freshwater methanogen Methanosarcina barkeri is known to utilize hydrogenases to transfer electrons from ferredoxin to methanophenazine. This thesis characterizes electron transport in the marine methanogen Methanosarcina acetivorans. Genomic comparison of hydrogenase gene clusters …
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Bioenergetics of Methanococcus voltae: Possible role for a primary sodium pump in ATP formation
… been obtained in whole cells of Methanosarcina barkeri that ATP is synthesized via a chemiosmotic mechanism. Results obtained with protoplasts from Methanobacterium thermoautotrophicum also agree with a chemiosmotic mechanism of ATP synthesis. Lancaster proposed a mechanism of ATP formation in …
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Structural Characterization of the Transfer Ribonucleic Acids From Halobacterium Volcanii and Other Archaebacteria
… voltae, Methanomicrobium mobile, Methanosarcina barkeri, Thermoplasma acidophilum, and Sulfolobus acidocaldarius), as analyzed by two-dimensional thin layer chromatography of the ('32)P-labelled nucleotides. Second, we describe the sequences of seventeen H. volcanii tRNAs (tentative for two) as …
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Growth and Survivability of Microorganisms at Martian Temperatures and Pressures
… of four species of methanogens (Methanosarcina barkeri, Methanobacterium formicicum, Methanococcus maripaludis, and Methanothermobacter wolfeii) to survive and/or grow under 1) low-pressure conditions and 2) freeze/thaw cycles. Low pressure studies include both survival and active growth …
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Orthogonal pair-directed codon reassignment as a tool for evaluating the factors affecting translation in E. coli
… jannaschii (M. jannaschiiI) and Methanosarcina barkeri (M. barkeri) to measure the in vivo reassignment efficiency of 30 different sense codons to tyrosine in E. coli with a simple fluorescence-based screen. The suite of sense codon reassignment efficiencies identified multiple promising codons …
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Organic geochemical biosignatures in alkaline Hydrothermal ecosystems
… of products of the methanogen Methanosarcina barkeri and found that fractionation varied strongly with substrate availability.These results inform our analysis of methanogen lipids from carbonates of the Lost City Hydrothermal Field. This ultramafic ecosystem produces methane highly enriched …
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Mechanisms of Methanogenic Inhibition in Advanced Anaerobic Digestion
… community of the aceticlastic Methanosarcina barkeri in MAD to the hydrogenotrophic Methanoculleus bourgensis in THD. A similar shift in methanogenic community was observed between low temperature thermophilic digestion at 47°C, where the dominant order was Methanosarcinales, to high …
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Mechanistic investigations of class III anaerobic ribonucleotide reductases
… rather than from NADPH. The Methanosarcina barkeri class III RNR was cloned and expressed, and found to catalyze nucleotide reduction using a system involving Fdx, NrdH and a [4Fe4S] protein ferredoxin:thioredoxin reductase. The diversity of reducing equivalents used for anaerobic …
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Computer-assisted generation and curation of genome-scale metabolic models with case studies in the methanogen genus Methanosarcina
… Methanosarcina acetivorans and Methanosarcina barkeri. Methanosarcina species are particularly attractive model organisms for methanogenesis due to their wide substrate utilization capabilities (compared to other methanogens): the diversity in metabolic capabilities for these organisms enables …
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Insights into substrate utilization and transcriptional regulation in methanosarcina
… by syntrophic associations. Previously, an M. barkeri mutant that had the capacity to use pyruvate as substrate was isolated. Chapter 2 of this dissertation is focused towards understanding the molecular basis of pyruvate utilization in these cells. Genetic and transcriptomic studies on two …
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Genetic Code Expansion in Mammalian Cells
… pair was quickly supplanted by Methanosarcina barkeri (Mb) and Methanosarcina mazei (Mm) pyrrolysyl-tRNA synthetase/tRNA pairs (PylRS/tRNAPyl), which are easily engineered to incorporate a wide variety of useful non-canonical amino acids. The developments in the genetic code expansion in …
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Diversidade de bactérias endofíticas em frutos de café
… epidermis/oidium (LEM BV41), Microbacterium barkeri (LEM CA26 e LEM BV37), Microbacterium luteolum (LEM CA01), Pectinobacterium carotovorum/carotovorum (LEM CA30, LEM CV35, LEM BV38, LEM BA43 e LEM BA45), Pseudomonas putida biótipo A (LEM BA47), Salmonella typhymurium GC grupo B (LEM CV19), …
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Improving Methanosarcina genome-scale models for strain design by incorporating cofactor specificity and free energy constraints
Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-04-22T20:19:19Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Richards_Matthew.docx: 566731 bytes, checksum: 34f812c17c0944f31f7b5fba2a34d536 (MD5) Richards_Matthew.pdf: 1474395 bytes, …