Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Baculoviruses"”.
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Use of baculovirus surface display for characterization of AHSV4-VP2 antigenic structure
… protein vaccines expressed from recombinant baculoviruses are an option for generating safe vaccines due to the absence of pre-existing immunity, non-toxicity in mammalian systems and their inability to replicate in mammalian cells. In addition, the fact that AHSV replicates in insect cells …
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Host specific gene expression of the baculovirus, Spodoptera frugiperda nuclear polyhedrosis virus, in insect cell lines
Baculoviruses (family: Baculoviridae) infect insects with high lethality and specificity and are experimental insecticides for suppressing pest populations in the field. Recently, recombinant DNA constructions of baculoviruses have been successfully utilized for the expression of foreign genes. In …
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The dual role of lef-2 in DNA replication and late gene expression during baculovirus-replication
… between examples of the gene from 37 different baculoviruses. Mutation of four of these cysteines into serines and construction of recombinant viruses showed that budded virus production was reduced from 0 — 48% of that seen in AcMNPV controls. This suggests an important role for the cysteine …
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RNA transmission and expression from inert HIV candidate vaccine virus-like-particles
… and expression of genes from recombinant baculoviruses and other Gp64 pseudotyped live viruses in mammalian cell lines both in vivo and in vitro. This study, therefore, set out to establish for the first time whether inert, baculovirus-derived (Gp64 pseudotyped) Gag VLPs could mediate …
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Characterisation and expression of ORF2 in genome segment 10 of African horse sickness virus
… in insect and mammalian cells. Recombinant baculoviruses were confirmed to express ORF2 or ORF2-eGFP. Localisation of ORF2-eGFP was specific within the nucleus and cytoplasm of Sf9 cells. ORF2 transiently expressed in BSR-T7 cells initially localised in the cytoplasm and later moved to the …
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The analysis of Autographa Californica multiple Nucleopolyhedrovirus EXONO (ORF141) function and its role in virus budding
Baculoviruses have a biphasic replication cycle producing two types of virions, budded virus (BV) and occlusion derived virus (ODV) which are required for the systemic spread or oral infection with the insect host respectively. Little is known about the events of the BV pathway and the mechanism by …
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Baculovirus inhibitors of apoptosis
… unique amongst the iap homologues identified in baculoviruses other than AcMNPV. Recombinant HzSNPV deficient in the production of iap was unstable and could not be isolated from the parental virus. The role of this gene in the infection process of HzSNPV remains unclear. Recombinant AcMNPV …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
… bacterial, insect or yeast expression systems. Baculoviruses yielding high expression of each component of WRC were obtained using a modified pFastBac vector, where translation is driven by the lobster tropomyosin promoter. Co-infection into Sf9 cells allowed efficient expression and …
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Maintaining the balance: persistent baculovirus infection in insect cells
Baculoviruses such as Autographa californica nucleopolyhedrovirus (AcMNPV) are insect-specific viruses that usually kill the host. However, many species harbour persistent, non-lethal covert baculovirus infections although these have rarely been observed in vitro. Hence, little is known about the …
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Toxicity of mycotoxins to insects and underlying molecular and biochemical mechanisms
… b5 in Sf9 insect cells infected with recombinant baculoviruses. Assays conducted with 16 compounds, including AFB1, showed that CYP6AB11 can efficiently metabolize imperatorin (0.88pmol/min/pmol) and slowly metabolize PBO (0.11 pmol/min/pmol). In view of all of results, it is clear that similar …