Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 77 for “"Baculovirus"”.
-
Baculovirus inhibitors of apoptosis
… (iap) sequences identifted a RING finger and baculovirus IAP repeat (BIR) conserved in all the members of this protein family. The predicted sequence of the HzSNPV iap was found to be 42% identical to that of Orgyia pseudosugata (0p) MNPV iap and 39% identical to that of Cydia pomenella (Cp) …
-
Maintaining the balance: persistent baculovirus infection in insect cells
Baculoviruses such as Autographa californica nucleopolyhedrovirus (AcMNPV) are insect-specific viruses that usually kill the host. However, many species harbour persistent, non-lethal covert baculovirus infections although these have rarely been observed in vitro. Hence, little is known about the …
-
Baculovirus stability in serum-free lyophilized and wet storage conditions
The baculovirus expression vector system (BEVS) is an effective way to produce recombinant proteins for biopharmaceuticals. However baculovirus stocks are stored in subzero temperatures to maintain virus stability, and fetal bovine serum is commonly used in the storage solution. In an effort to …
-
Baculovirus as a gene delivery and expression vector in mammalian cells
… such as BacMam gene transfer technology (Baculovirus-based expression in mammalian cells) is an attractive option for reducing I/R injury due to its high safety profile compared to mammalian viruses. However, limiting factors, including the requirement of high-doses of viral vector and low …
-
Use of baculovirus surface display for characterization of AHSV4-VP2 antigenic structure
… protein vaccines expressed from recombinant baculoviruses are an option for generating safe vaccines due to the absence of pre-existing immunity, non-toxicity in mammalian systems and their inability to replicate in mammalian cells. In addition, the fact that AHSV replicates in insect cells …
-
Isolation and characterisation of extracellular vesicles in baculovirus infection of insect cells
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-specific virus. Its infection can be studied in Spodoptera frugiperda. It has a double-stranded DNA genome and a biphasic lifecycle, producing budded virus (BV) to infect neighbouring cells and occlusion …
-
Gastric H+,K+-ATPase. Functional synthesis in vitro using recombinant baculovirus mediated expression
Contains fulltext : mmubn000001_215741269.pdf (Publisher’s version ) (Open Access)
-
Baculovirus surface display of influenza virus haemagglutinin and its potential as a vaccine
… pandemic influenza. Sub-unit vaccines based on baculovirus surface display technology are an option for the development and generation of safe human vaccines due to the lack of pre-existing immunity, toxicity and ability to replicate in mammalian systems. Furthermore, this technology may provide …
-
Baculovirus-directed expression of the phosphorylase kinase catalytic subunit: pseudosubstrate and calmodulin regulation
Phosphorylase kinase (EC 2.7.1.38) is a key enzyme involved in the regulation of the glycogenolysis pathway. It catalyzes the Ca²⁺-dependent phosphorylation and activation of the enzyme glycogen phosphorylase to make the active form glycogen phosphorylase. Phosphorylase kinase is composed of 4 …
-
Taking a closer look: exploring the functional roles of P10 in baculovirus-infected cells
P10 is a small, highly expressed baculovirus protein whose function continues to be a mystery. Believed to be non-essential during virus infection, P10 is associated with a number of intracellular structures with suggested roles in polyhedron formation, nuclear stability and lysis. The work …
-
Regulation of late and very late gene expression in both lytic and persistent baculovirus infections
… proteins (e.g. vaccines). However, persistent baculovirus infections are also known. They are maintained throughout the life cycle of the host without causing mortality and insect liquefaction for polyhedra dissemination. The molecular mechanism of how the fine balance of host and virus gene …
-
Host specific gene expression of the baculovirus, Spodoptera frugiperda nuclear polyhedrosis virus, in insect cell lines
Baculoviruses (family: Baculoviridae) infect insects with high lethality and specificity and are experimental insecticides for suppressing pest populations in the field. Recently, recombinant DNA constructions of baculoviruses have been successfully utilized for the expression of foreign genes. In …
-
The dual role of lef-2 in DNA replication and late gene expression during baculovirus-replication
… between examples of the gene from 37 different baculoviruses. Mutation of four of these cysteines into serines and construction of recombinant viruses showed that budded virus production was reduced from 0 — 48% of that seen in AcMNPV controls. This suggests an important role for the cysteine …
-
Reconstructing the gamma-secretase complex using the MultiBac baculovirus system for expression of heterologous multi-protein complexes
… that has been devised to generate recombinant baculovirus DNA that can be used to express multi-subunit protein complexes. The basis of the system is the use of transfer vectors to facilitate the assembly of polycistronic expression cassettes in the pFBDM vector, ultimately generating one …
-
Gene regulation in baculovirus-infected insect cells : application in the development of a budded virus free expression system
The baculovirus expression vector system (BEVS) is a valuable tool for the production of recombinant proteins including complexes such as virus-like particles (VLPs) for vaccine production. A significant limitation of the BEVS, however, is the co-production and subsequent contamination of VLPs and …
-
The Penaeus monodon baculovirus (MBV): Its epizootiology, prevention and control in penaeid shrimp hatcheries and grow-out ponds in the Philippines.
The prevalence of Penaeus monodon baculovirus (MBV) in black tiger prawn (Penaeus monodon Fabricus) was investigated based on 372 diagnostic cases examined from October 1989, to December, 1990. Laboratory transmission of MBV to the different life stages of P. monodon and tolerance of MBV in …
-
Generation of Baculovirus-Brucella Abortus Heat Shock Protein Recombinants; Mice Immune Responses Against the Recombinants, and B. Abortus Superoxide Dismutase and L7/L12 Recombinant Proteins
… against these proteins is currently unresolved. Baculovirus recombinants were generated in <i>Sf9</i> insect cells for <i>B. abortus</i> HSPs and the protein expression was optimized. Humoral (Western blot), cell mediated (CMI, IFN-g- release by splenocytes, and CD3+CD4+, CD3+CD8+ T cell/ total …
-
RNA transmission and expression from inert HIV candidate vaccine virus-like-particles
… Gag VLPs produced in insect cell-based, baculovirus expression systems have been observed to incorporate the baculovirus-derived Gp64 envelope glycoprotein. Gp64 has been shown to be efficient at enabling the delivery and expression of genes from recombinant baculoviruses and other Gp64 …
Page 1 of 4