Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Bacteroides thetaiotaomicron"”.
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Enzymes involved in utilization of pullulan by Bacteroides thetaiotaomicron
A pullulanase I gene from Bacteroides thetaiotaomicron has been cloned. To determine whether the cloned pullulanase gene was essential for pullulan utilization, I used directed insertional mutagenesis to inactivate the B. thetaiotaomicron pullulanase gene.
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Molecular characterization of the starch utilitization system of Bacteroides thetaiotaomicron
Bacteroides spp. are obligately anaerobic Gram-negative bacilli that reside in high numbers in the colons of humans and animals. It has been estimated that Bacteroides spp. constitute as high as 30% of all colonic isolates. Since mono- and di-saccharides are efficiently absorbed in the small …
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Genetic characterization of the mucopolysaccharide utilization system of Bacteroides thetaiotaomicron
… that some of the major colonic species such as Bacteroides thetaiotaomicron can utilize host-derived polysaccharides as well as dietary polysaccharides raised the question of whether host-derived polysaccharides might be an important source of polysaccharides for some colonic bacteria. To …
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Functional Characterization of Proteins Involved in Starch Utilization by Bacteroides Thetaiotaomicron
Bacteroides spp. are gram-negative obligate anaerobes, constitute 25--30% of the human colonic microflora, and are proposed to be the major polysaccharide fermenters in the colon. We believe that Bacteroides degrades these large substrates by binding them on the cell surface and hydrolyzing them to …
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The regulation and two genes in Bacteroides thetaiotaomicron starch utilization system
Bacteroides thetaiotaomicron, a gram-negative obligate anaerobe, appears to utilize starch by first binding the polymer to its surface, then translocating it into the periplasmic space. Several genes have been identified that encode enzymes or outer membrane proteins involved in starch utilization. …
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Outer Membrane Proteins Associated With Utilization of Chondroitin Sulfate by Bacteroides Thetaiotaomicron
Bacteroides thetaiotaomicron employs a cell-associated inducible system to degrade chondroitin sulfate (CS), a mucopolysaccharide that may be a natural carbon source for this organism in the colon. The system that V. thetaiotaomicron uses to break down CS is not fully understood. Chondroitin lyase, …
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Genetic Analysis of Chondroitin Sulfate Utilization in Bacteroides Thetaiotaomicron (Cloning, Mutagenesis, Polysaccharide)
… to develop techniques for genetic analysis of Bacteroides thetaiotaomicron, a human colonic bacteria, which could be applied to determining the organization and regulation of the genes involved in the utilization of chondroitin sulfate, a complex polysaccharide which is probably a source of …
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DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
I implemented a biochemical approach utilizing heparin column, size-exclusion and DNA-affinity chromatography, 2-D gel electrophoresis and mass fingerprinting to identify proteins that bound the attachment sites of CTnDOT (attDOT ) and NBU1 (attNI), and stimulated integration in vitro for both DNA …
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Analysis of the ammonium assimilation pathways of the human colonic bacterium, bacteroides thetaiotaomicron
… preferred nitrogen source for microbial growth. Bacteroides thetaiotaomicron, a model organism for human gut Bacteroidetes, encodes genes for the capture of ammonium through the two primary pathways, the glutamate dehydrogenase (GDH) pathway and the glutamine synthetase/glutamate synthase …
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Interactions Among the Outer Membrane Proteins of the Starch Utilization System of Bacteroides Thetaiotaomicron
In this study, the minimal starch utilization system was used to study interactions among SusC, SusD, and SusG. To examine interactions between SusE and SusF, wild type and other mutants were used. For this study, several biochemical techniques such as formaldehyde cross-linking, affinity …
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Characterization of the structure and function of a <I>Bacteroides thetaiotaomicron</I> 16S rRNA promoter
The bacteroides group is a subdivision in the <I>Cytophaga-Flavobacterium-Bacteroides</I> phylum. This group is as phylogenetically distinct from other Gram-negative enterics, including <I>Escherichia coli</I>, as they are from Gram-positive organisms. Furthermore, there is no cross expression …
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Regulation of Bacteroides chondroitin sulfate utilization genes
… chondroitin sulfate utilization (csu) system in Bacteroides thetaiotaomicron as the model system to study regulation.
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Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae
… influenzae Rd K20, Arabidopsis thaliana and Bacteroides thetaiotaomicron VPI-5482 were expressed in S. cerevisiae to produce a strain capable of metabolising D-xylose (a biomass constituent). Resulting strains were analysed for protein production, growth differences and xylose isomerisation …
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Mutational Analysis of NBU1 Attachment Sites Reveals a Novel Recombination Mechanism
… is normally integrated in the chromosome of its Bacteroides host. Bacteroides are the predominant member of the human intestinal tract microflora, and are opportunistic pathogens that can cause life-threatening infections. A recent survey of Bacteroides isolates has shown an increase in …
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Discovery of Function in the Enolase Superfamily
… hutchinsonii (k cat/KM of 5.5 x 104 M -1s-1) and Bacteroides thetaiotaomicron (kcat/KM of 5.8 x 104 M-1s-1), respectively. Computational predictions, provided by Prof. Matthew Jacobson's laboratory at UCSF, were proven correct for both enzymes. In collaboration with Prof. Steven Almo's laboratory …
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Genetic Manipulation of Bacteroides for Transient Colonization of Germ-free Mice
… and D-Alanine (D-Ala) in a species of interest, Bacteroides thetaiotaomicron. These amino acids are incorporated into the peptidoglycan, or cell wall, of most bacteria, like B. thetaiotaomicron. This study builds on concepts from a previously developed E. coli strain called HA107, where …
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Variability in fecal microbial drug deglucuronidation
… of the three healthy donors. Only the isolates Bacteroides thetaiotaomicron, Bacteroides uniformis, Bacteroides vulgatus, Lachnospira pectinoshiza, and a species from the Luoshenia genus were found to have deglucuronidation activity in the 4-MUG and PNPG assays. Variability in reaction rates and …
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