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Showing 1 to 20 of 34 for “"Bacteroides fragilis"”.
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DNA repair in bacteroides fragilis
Bacteroides fragilis is a gut commensal in both humans and animals where it benefits the host through metabolizing indigestible compounds, stimulating the immune system and protecting against pathogen colonization. However, it is also an opportunistic pathogen, responsible for approximately half of …
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Glutamine synthetase in Bacteroides fragilis
Bactereroides fragilis is a gram-negative, non spore-forming, obligate anaerobe of the human intestinal microbiota. It is, however, an opportunistic pathogen and has been ranked as the most prevalent isolate in cases of anaerobic septicaemia. Similar to most bacteria, ammonium is assimilated in B. …
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Molecular genetic studies of bacteroides fragilis
Some genetic systems operative in Bacteroides fragilis have been successfully investigated. Firstly the bacteriocin produced by the B.fragilis BF-1 strain was purified and partially characterized. This bacteriocin was found to be cell bound and constitutively produced by the bacteria. The purified …
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DNA repair in Bacteroides fragilis Bf-2
Repair deficient mutants of Bacteroides fragilis have been isolated in order to study the responses of this organism to various DNA damaging agents at the physiological and molecular levels. Two types of mutants were isolated by ethyl methane sulphonate mutagenesis of B.fragilis followed by …
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The effect of metronidazole on Bacteroides fragilis and Escherichia coli
… including those caused by the anaerobic pathogen Bacteroides fragilis. Metronidazole is an inert substance that requires reductive activation to become cytotoxic. In its activated form metronidazole induces DNA damage. Relatively little is known about the cytotoxic effects of this drug in vivo. …
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Characterization and Molecular Analysis of Fragilysin: The Bacteroides fragilis Toxin
Bacteroides fragilis is a gram negative, anaerobic rod, that is a member of the normal colonic microflora of most mammals, and it is the anaerobe most commonly isolated from human soft tissue infections. During the past decade, strains of B. fragilis that produce an enterotoxin have been implicated …
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Cloning and expression of cambialistic Bacteroides fragilis superoxide dismutase gene
… from a LambdaGEM-11 genomic library of <i>Bacteroides fragilis</i>. In order to generate a complete genomic library, <i>B. fragilis</i> genomic DNA was partially digested with the restriction endonuclease Sau3AI and was ligated to cloning vector, LambdaGEM-11. After in vitro packaging, DNA …
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Role and regulation of glutamate dehydrogenase activity in Bacteroides fragilis bf1
Bacteroides fragilis is a gram-negative obligate anaerobe that exists normally in the human large intestine. It is, however, capable of causing a variety of infections once outside of this environment. Previous studies have suggested that the regulation of the ammonia assimilatory pathways in B. …
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Crystal structure of the Large Type III Glutamine Synthetase from Bacteroides Fragilis
… GS, GlnN, from the opportunistic human pathogen, Bacteroides fragilis, with a view to better understanding the GSIII family in the light of the known structure functionrelationships of the other GS enzymes, and to investigate the potential for the design of selective inhibitors against the …
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The molecular characterisation of the recA locus in the opportunistic pathogen Bacteroides fragilis
Bacteroides fragilis is a human gut commensal and an opportunistic pathogen causing anaerobic abscesses and bacteraemias which are treated with the drug, metronidazole, a DNA damaging agent. The RecA protein is thought to be involved in the repair of metronidazole damage as well as damage caused by …
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Serological and chemical studies on the antigens of Bacteroides Fragilis and related species
… properties of antigens extracted from strains of Bacteroides fragilis and related species belonging to several different DNA homology groups were investigated. Antisera prepared against formalin-treated whole cells suspensions of representative strains were tested against cell suspensions, cell …
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Metronidazole resistance in clinical Bacteroides fragilis isolates from Groote Schuur Hospital, Cape Town, South Africa
Bacteroides fragilis, an anaerobic gut commensal and opportunistic pathogen, is a leading cause of anaerobic abscesses and bacteraemias. Treatment of infections is complicated by the emergence of resistance to several of the antibiotics, specifically metronidazole, used in the clinical setting. The …
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Effects of far ultra-violet radiation and oxygen on macromolecular synthesis and protein induction in Bacteroides fragilis Bf-2
… and viability in the obligate anaerobe, Bacteroides fragilis, as well as the specific proteins induced in this organism by these different DNA damaging agents.
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Bacteroides fragilis outer membrane vesicles are used for secretion of a discrete subset of bacterial RNAs that stimulate an immune response in colonic epithelial cells.
… strain (NTBF) and a pathogenic strain (ETBF) of Bacteroides fragilis. To distinguish the differences in the sRNA profiles of both strains and their OMVs, we conducted small RNA-seq and identified enrichment of discrete sRNA species in OMVs that were also differentially expressed between the two …
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Characterization of TraM, a Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts with the B. Fragilis Putative Coupling Protein Bcta and the B. Fragilis Relaxase Protein BmpH
<p><italic>Bacteroides</italic> spp. organisms, the most prominent bacteria of the human colon, are reservoirs of antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The <italic>B. fragilis</italic> clinical isolate LV23 harbors a 37kb conjugative …
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Experimental anaerobic bacterial infections in mice
… development of a technique for producing a pure Bacteroides fragilis infection in mice is described. The infection produces large subcutaneous abscesses at the site of bacterial injection in approximately 90% of the mice. The abscess can be seen as an obvious swelling within 5-7 days …
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Bacteriodes fragilis interactions with the collagen type I component of the host extracellular matrix
Bacteroides fragilis is an opportunistic pathogen which can cause life threatening infections in humans and animals. The increasing incidence of antibiotic resistance by the bacterium has prompted the search for novel therapeutic targets. The idea of antivirulence over antimicrobial drug …
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Liquid Chromatography -- Tandem Mass Spectrometry Techniques for Drugs of Abuse as Applied to Bacterial and Forensic Toxicology
… to investigate the effects of Escherichia coli, Bacteroides fragilis, and Clostridium perfringens on drugs of abuse, and to compare these metabolites to those produced during normal human metabolism. To analyze and quantify these effects, a novel liquid chromatography triple quadrupole tandem …
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Synthesis and Evaluation of Biological Activity of a Potential Immunomodulatory Zwitterionic Polysaccharide
<p>The gram-negative anaerobic bacterium <em>Bacteroides fragilis</em> is an integral component of the normal gastrointestinal flora. The bacterium colonizes the intestinal tract of human beings as it has no reservoir other than mammals. An unprecedented proportion of the genomic DNA of <em>B. …
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