Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Bacteroides conjugative transposon (CTnDOT)"”.
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Excision of the Bacteroides Conjugative Transposon CTnDOT
In this study, we showed that expression of the exc operon is regulated by tetracycline and mediated by the regulatory proteins, RteA, RteB, and RteC. Tetracycline induces the expression of rteA and rteB, genes in the tetQ operon. Then, RteB induces the expression of rteC. RteC, subsequently, …
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Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT
… of rteC. In order to determine whether CTnDOT regulatory genes can affect the expression of chromosomal genes in B. thetaiotaomicron , I used GeneChip and quantitative RT-PCR analyses. I showed that RteA and RteB are global regulators that induce the expression of various genes, in …
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The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
… transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence …
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Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT
Bacteroides spp. are a primary inhabitant of the human colon, and play an important role in the health of the human host as Bacteroides are important for nutrient acquisition, the breakdown of starches that would otherwise go undigested, and the management of relevant pathogens such as C. …
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Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT
Many human colonic Bacteroides spp. harbor a conjugative transposon (CTn) called CTnDOT. CTnDOT carries two antibiotic resistance genes tetQ and ermF. A distinctive feature of CTnDOT is that both its excision and transfer are stimulated by tetracycline. The regulation of excision has been described …
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Positive regulators of Bacteroides CTnDOT excision and transfer
… stimulates both excision and transfer of a Bacteroides Conjugative transposon CTnDOT. It was shown previously that a gene, rteC, is necessary for tetracycline-stimulated transcriptional regulation of the xis2c operon, which contains the excision genes. The protein encoded by this gene, RteC, …
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Role of the core in CTnDOT integration
IntDOT is the tyrosine recombinase encoded by the Bacteroides conjugative transposon CTnDOT. It catalyzes integration and excision into and out of the bacterial host chromosome. Although it is a member of the well studied tyrosine recombinase family, IntDOT is of particular interest because it can …
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The Role of Bacteroides Conjugative Transposons in the Spread of Antibiotic Resistance Genes, And, The Role of recA in DNA Damage Repair in Deincoccus Radiodurans
… projects. The first project involves using Bacteroides species, which are normal residents of the human colon, as a model for investigating the horizontal transmission of antibiotic resistance genes in the environment. The data presented in this thesis shows that an open genetic conduit …
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Characterization of IntDOT, the CTnDOT Integrase
… analysis revealed that the integrase of the Bacteroides conjugative transposon CTnDOT (IntDOT) might be a member of the tyrosine recombinase family because IntDOT has five of six highly conserved residues found in the catalytic domains of tyrosine recombinases. Yet, IntDOT catalyzes a …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… recombination reactions that promote movement of CTnDOT that is an integrative conjugative element (ICE) found in Bacteroides spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require sequence identity in the region where …