Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Bacteriophage T7"”.
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Simulation, models, and refactoring of bacteriophage T7 gene expression
… such models for gene expression during bacteriophage T7 infection. T7 gene expression is a particularly well suited model system because knowledge of how the phage functions is thought to be relatively complete. My work focuses on two questions in particular. First, can we address …
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Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators
… the mechanism of transcription termination by bacteriophage RNA polymerases, templates encoding variants of the transcription-termination structure in the regulatory region of the threonine (thr) operon of Escherichia coli were used. The thr terminator comprises two distinct structural …
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
… the degradation of mRNA, and the maturation of T7 phage polycistronic early, middle, and late mRNAs. Bacteriophage T7 expresses a protein kinase (T7 PK), which is cAMP-independent, serine/threonine-specific, and phosphorylates over 90 cellular proteins, including RNase III during infection. …
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Gene Expression Control for Synthetic Patterning of Bacterial Populations and Plants
… In both model systems, I explore the use of bacteriophage T7 RNA polymerase as a gene expression engine to control synthetic patterning across populations of cells. In E. coli, I developed a ratiometric assay of bacteriophage T7 RNA polymerase activity, which I used to systematically …
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Single molecule fluorescence studies of replicative helicases
… including replication, recombination and repair. Bacteriophage T7 gp4 serves as one of the model proteins for replicative helicases. Moreover, the replication machinery of Bacteriophage T7 provides a simple model to study the dynamics of DNA replication. To address how the leading and lagging …
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Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
… the intergenic region of the single stranded DNA bacteriophage f1 and either a bacteriophage SP6 or T7 promoter have been constructed. Coinfection of E. coli with these chimeric plasmids and a single stranded DNA helper phage results in the replication and secretion of the pSP6(.)f1 or pTZ …
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Control Method for Invasive Aquatic Species introduced via Ballast Water: Effects of Carbon Dioxide Supersaturation on Survivorship of Digesia tigrina (Planaria: Maculata) and Lirceus brachyurus (Isopoda: Crustacea) and Effect of High Hydrostatic Pressure Processing on Freely Suspended and Shellfish Associated T7 Bacteriophage
… on Freely Suspended and Shellfish Associated T7 Bacteriophage The effectiveness of hydrostatic pressure processing (HPP) for inactivating viruses has only been evaluated in a limited number of studies and most of the work has been performed with freely suspended viruses. In this work, the …
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Biochemical mechanisms for selectivity governing RNA and DNA replication
… are well known. The DNA polymerase from bacteriophage T7 has been a model system for understanding high-fidelity DNA replication, yet outstanding questions remain about the controversial nucleotide induced conformational change. I developed methods to directly measure the conformational …
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Studies of Plant Viroid RNA and Other RNA Species of Unusual Function
… Fingerprints of human 5S RNA and bacteriophage f2 RNA resemble those of their noniodinated counterparts both in complexity and in specific pattern. Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues. This iodination …
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Sturcture and function of tomato ringspot virus RNA1 and RNA2
… RNA1 and RNA2 were constructed and fused to the bacteriophage T7 RNA promoter. RNA transcripts synthesized from constructs in vitro using 17 RNA polymerase were tested for infectivity by inoculation onto Chenopodiunt amaranticolor and assaying for viral symptoms. Transcripts were found to be …