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Showing 1 to 9 of 9 for “"Bacteriophage Mu"”.
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Organization and dynamics of the bacteriophage Mu transpososome
… of a linear mobile genetic element, such as the bacteriophage Mu, requires a set of spatially and temporally coordinated DNA phosphoryl transfer reactions to move the element from an existing DNA location to a new DNA site. The Mu transpososome, where the DNA cleavage and joining reactions that …
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Studies On The In Vitro Transposition Of Bacteriophage Mu Dna
The early steps in the transposition of Mu DNA have been examined in vitro using purified Mu A, Mu B and E. coli HU proteins, a mini-Mu donor plasmid and target DNA. Two stable protein-DNA complexes, or transpososomes, have been characterized. Type 1 complex formation requires a supercoiled mini-Mu …
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Interaction of Bacteriophage Mu Middle Transcription Activator Protein Mor with Promoter DNA
<p>Gene expression during lytic development of bacteriophage Mu is regulated by a</p> <p>transcriptional cascade in three phases: early, middle and late. Transcription from the middle promoter Pm requires the 129-amino acid transcriptional activator Mor, a product of early transcription, and the …
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Dynamics of protein-DNA interactions in a Mu transpososome
… for the life-cycles of many viruses, from bacteriophage Mu to HIV; it is spreading antibiotic resistances between bacterial populations; and it is responsible for spontaneous mutations in all the kingdoms of life. Transposition is mediated by a protein, the transposase, encoded by the …
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Role of communication between subunits and enzymes in ClpXP-mediated substrate unfolding and degradation
… have been studied extensively, the role of communication between subunits within a ClpX hexamer or between enzymes in ClpXP has not been addressed. I initially screened numerous ClpX point mutants for their ability to support host-cell lysis by bacteriophage Mu, and then purified several …
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Transposable prophage Mu exists as an independent chromosomal domain in E. coli
… proteins. We find that transposable prophage Mu, transcriptionally silent by definition, is organized into an independent domain as determined by the close proximity of Mu termini L and R separated by a 37 kb Mu genome. Cre-loxP recombination is used in this study in vivo and in vitro. …
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DNA Binding Specificity of Mu Transcription Factor C and Crystallization of C : DNA Complex
The lytic cycle of phage Mu is regulated by a transcriptional cascade consisting of early, middle and late transcription. The Mor protein is an activator of the middle promoter Pm and is encoded by the last gene of the early transcript. The C protein is an activator of the four late promoters Plys, …