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Showing 1 to 12 of 12 for “"Bacterioferritin"”.
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Mechanisms of Iron Oxidation in the Ferroxidase Centre of Escherichia coli Bacterioferritin: A Comparison to Other Ferritins
… Fe2+ oxidation at the diiron centres in E. coli bacterioferritin have expanded upon the evidence that the protein exists to prevent oxidative stress. This was performed in novel anaerobic setups by directly measuring the protein’s reactivity with H2O2. UV/ vis spectroscopy highlighted that H2O2 …
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Mechanistic studies of iron uptake into, and release from, Ferritins
… suggesting a distinct mechanism of iron uptake. Bacterioferritin (BFR) is a unique heme-containing bacterial member of the ferritin family that stores up to 2700 iron ions as a ferric oxyhydroxide phosphate mineral within its central cavity. This core is surrounded by 24 identical protein …
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Evaluation of the Aging Immune System Using a Mouse Model of Brucella Infection
… magnitude of IgG1 and IgG2a response to bacterioferritin and SOD of Brucella. The data suggests that both Th17 as well as Th1 responses were accentuated in old mice as compared to young mice following infection with Brucella. How the Th17 and Th1 branches of immune system work together …
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Design, Structure, And Action Of An Artificial Photosynthetic Reaction Center
… similarity to cytochrome b at the heme site and bacterioferritin at the metal site. Transient absorption spectroscopy shows that the reduced state of heme B is stabilized in the presence of a tyrosine residue for 150 ms after light absorption at pH 9.5. The binding of ferrous iron extends the …
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Towards reverse engineering of Photosystem II: Synergistic Computational and Experimental Approaches
… using the protein scaffold of E. coli’s Bacterioferritin (BFR) existed. The oxidation potential of the pigment used for the BFR ‘reaction centre’ was measured and the protein effects calculated in a similar fashion to how P680 potentials were calculated in PSII. The BFR-RC’s pigment …
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Mechanisms of Oxidative Stress Resistance in Porphyromonas gingivalis W83
… hydroperoxidase subunit C <em>(ahpC)</em>and bacterioferritin comigratory protein <em>(bcp)</em> genes, DNA repair mechanisms and the unique hemin layer of <em>P. gingivalis. </em>We tested the physiological function of the isogenic <em>ahpC</em> and <em>bcp</em>mutants to hydrogen peroxide. …
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High-Throughput Determination of Mycobacterium smegmatis Protein Complex Structures
… structures of glutamine synthetase I, bacterioferritin, and Encapsulin. These structures were unambiguously identified through a combination of fitting of homologous crystal structures into the low-resolution maps, and information obtained by liquid chromatography mass spectrometry …
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KINETIC, THERMODYNAMIC AND MECHANISTIC FEATURES OF ESCHERICHIA COLI BCP, AN UNUSUALLY VERSATILE PEROXIREDOXIN
In <italic>Escherichia coli</italic>, bacterioferritin-comigratory protein (<italic>Ec</italic>BCP) by definition is a peroxiredoxin (Prx) which catalyzes the reduction of hydrogen peroxide and organic hydroperoxides. <italic>Ec</italic>BCP is a member of the most poorly characterized and least …
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Analyzing Extracellular Vesicles as Potential Biomarkers of Stroke Using Polymer Microfluidic Devices
… on identification of mutations stabilizing Bacterioferritin associated ferredoxin is included in Appendix.
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Microbial Diversity in the Human Mouth: Dominant Genera and their Interactions.
… absence of the bcp gene (encoding the peroxidase bacterioferritin co-migratory protein) and it was hypothesised that this was due to an up-regulation of other peroxidases in this mutant. Real-Time PCR (RT-PCR) experiments showed increased expression of prx in a bcp-deficient N. meningitidis mutant …
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The molecular characterisation of the recA locus in the opportunistic pathogen Bacteroides fragilis
… the B. fragilis bcp gene encoded a functional bacterioferritin co-migratory protein (BCP), which is a small thiol-specific protein with antioxidant properties. This BCP showed flexibility in its substrate preference with activity against H2O2, tet-butyl hydroperoxide and linoleic acid. The …
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Biogenesis of iron-sulfur clusters and intracellular iron metabolism
Iron-sulfur ([Fe-S]) clusters represent one of natures most diverse and ubiquitous protein prosthetic groups. [Fe-S] proteins are integral for diverse biological processes. Reconstitution of apo-proteins can occur by exogenously adding excess iron and sulfide. However, due to the toxicity of iron …