Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Bacterial cultures"”.
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Modification of the Next Generation Impactor and Incorporation of Bacterial Cultures as an In Vitro Pneumonia Model
… parameters of an antibiotic solution and antibacterial effects. Amberlite® beads were incorporated into the model to absorb the antibiotic so that time kill studies could be performed. Ceftazidime was chosen as the aerosolized antibiotic formulation due to its broad spectrum of activity as a …
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Towards real-time monitoring of bacterial cultures without the need for physical sampling: elastic scattering, fluorescence and Raman spectroscopy of Escherichia coli cultures.
… real-time, noninvasive, quantitative analysis of bacterial cultures for cell count and glucose uptake using turbidity corrected spectroscopy and Raman. We hypothesize that a Raman feature we ascribe to “biomass” (~1060 cm-1) i.e. a υ(C-O-C) asymmetric stretch or (C-C) stretch, and a glucose peak …
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Monitoring Bacteria Cultures Using Near Infrared (nir) Binary Spectronephelometry (bsn), Raman Spectra and Principal Component Analysis (pca)
… media components that define the state of native bacterial cultures, because of changing turbidity. A new technique, binary spectronephelometry (BSN) has the same or better sensitivity and precision for population monitoring as optical density at 600nm (OD600), while simultaneously measuring …
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Sanitizer efficacy against bacteria attached to synthetic meat processing surfaces
… in sterile poultry slurries were inoculated with bacterial cultures, agitated (100 rpm) for 2 hr at 18°C, and then incubated for 22-28 hr at 26°C (Shw. putrefaciens and P. fragi) or 37°C (S. typhimurium and L. monocytogenes). Scanning electron microscopy (SEM) was employed to determine …
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Optimization of Growth Conditions of Selected Exo-Polysaccharides Producing Lactic Acid Bacteria in Whey Mineral Concentrate
… view to optimize the growth of two EPS-producing bacterial cultures (Lactobacillus helveticus ATCC 15807 and Lactococcus lactis subsp. cremoris) in whey mineral concentrate (WMC). The parameters studied were TS%, incubation temperature, overnight cooling, incubation time, pH, and the effects of …
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Creation and validation of an enhanced in vitro model of the lungs
… Impactor (NGI) to allow incorporation of bacterial cultures for evaluation of formulation efficacy. The regular collection cup in the stage 4 of the NGI was replaced with a deep collection cup for incorporating bacterial cultures without affecting the critical impaction distance. Various …
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Rapid molecular signature analysis of pseudomonas aeruginosa by direct nanomolar detection of quorum sensing and biomarker molecules at a boron-doped diamond electrode
… the production of signaling molecules of PA14 bacterial cultures was performed using CE-UV after MCX SPE. Instant feedback and detection is important in a clinical setting which is a challenging objective. Investigations into the simultaneous electrochemical detection of these microbial …
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Gas chromatographic analysis of amines and other compounds produced by several species of Clostridium
… of amines and other compounds produced in bacterial cultures. Whole cultures were grown in six mls of chopped meat-glucose medium, acidified, and extracted with ethyl ether or chloroform to obtain organic acids, alcohols, ketones, aldehydes, and other neutral compounds. The residual broth …
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Targeting Proteins of Aspergillus fumigatus for Monoclonal Antibody Production
… rPPIase, rEglC and rCatB were purified from bacterial cultures. rPPIase and rCatB were emulsified in TiterMax® Gold adjuvant (CytRx Corporation, Los Angeles, CA) and used to immunize BALB/c mice. The rPPIase emulsion elicited a robust immune response (high titers) which was confirmed via …
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Differential expression profiling of proteomes of pathogenic and commensal strains of Staphylococcus aureus using SILAC
… membrane and secreted, were extracted from the bacterial cultures and mixed in a 1:1 ratio. The relative abundance of proteins present in light and heavy labeled samples was determined using MS analysis. From a total of 151 differentially expressed proteins, 58 were found to be upregulated in …
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Comparison of an Ultrasonic and a Non-Ultrasonic Endodontic Irrigation Protocol: A Clinical Study
… teeth were randomly treated with NAI or PUI. Bacterial cultures were obtained from the canals prior to instrumentation and after irrigation protocol. These cultures were evaluated for growth after 7 days in enriched anaerobic medium. Statistical analysis was performed on data using the …
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Using blue light for bacterial inactivation
… To investigate the impact of the following on bacterial inactivation using a custom-built LED light module: i) exposure time; ii) sample distance; iii) bacterial concentration; iv) sample position on plate; v) incorporation of UV-resistance plasmids and also to conduct comparisons in …
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ΚΙΝΗΤΙΚΗ ΤΗΣ ΚΕΦΑΔΡΟΞΙΛΗΣ ΣΤΟ ΜΗΤΡΙΚΟ ΓΑΛΑ. ΕΠΙΔΡΑΣΗ ΣΤΗ ΜΙΚΡΟΒΙΑΚΗ ΧΛΩΡΙΔΑ ΤΟΥΣΤΟΜΑΤΟΦΑΡΥΓΓΑ ΚΑΙ ΤΟΥ ΕΝΤΕΡΟΥ ΣΤΟ ΝΕΟΓΕΝΝΗΤΟ ΠΟΥ ΘΗΛΑΖΕΙ
… FLORA OF BREAST-FED BABIES, THE AEROBIC BACTERIAL FLORA OF 60 NEWBORN BABIES WERE STUDIED. BABIES WERE DIVIDED IN TWO GROUPS, GROUP A CONSISTED OF 30 BREAST-FED BABIES BORN TO MOTHERS UNDER 1,0 G. OF ORAL CEFADROXIL ADMINISTERED B.I.D. FROM DAY 3 TO DAY 6 AFTER DELIVERY, WHEREAS GROUP B …
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Characterization of DNA-repair potential in deep subsurface bacteria challenged by UV light, hydrogen peroxide, and gamma radiation
Subsurface bacterial isolates obtained through the DOE Subsurface Science Program were tested for resistance to UV light, gamma radiation and H₂0₂. Some deep subsurface bacteria were resistant to UV light, demonstrating ≥1.0% survival at fluences which resulted in a 0.0001% survival level of E. …
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Mechanistic studies of iron uptake into, and release from, Ferritins
… site (site C) only previously found in non-heme bacterial ferritins. The mechanism of iron uptake in P. multiseries ferritin and the effect of substitution of residues at site C has been studied using rapid reaction kinetic methods. These revealed that oxidation in PmFTN is very rapid suggesting …
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Messa a punto di processi biotecnologici per la produzione di biocombustibili da matrici organiche di rifiuto
… IFA - Tulln, where mixed cellulolytic cultures were isolated from biogas plants while assessing the metabolic pathway leading to cellulose degradation and verifying their capability to grow on lignocellulose too, proving that on the long term such bacterial cultures could be used as …
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Applications of aerobic and anaerobic bacteria in the fields of biological degradation of contaminants and biological wastewater treatment
… (DDE) utilising pure bacterial cultures. The second investigation dealt with the degradation of DDE and polychlorinated Biphenyl’s (PCB’s) utilising anaerobic sediments and soils from New Zealand. The third investigation worked on the Granulation of anaerobic …
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Whole Genome Analysis of Escherichia coli and Pseudomonas aeruginosa Mixed Culture Biofilms Grown for 117 Days in Spaceflight
… genome comparison of these 117-day spaceflight bacterial cultures reveals minor changes within the genome as a result of long-term growth in space. This project shows a glimpse of the effect spaceflight has on the direction of microbial evolution of biofilms.
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