Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Bacterial Proteins"”.
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Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins
… autophagy. We show that monoubiquitinated proteins decorate the AVM during infection of promyelocytic HL-60 cells, endothelial RF/6A cells, and to a lesser extent, embryonic tick ISE6 cells. Importantly, tetracycline treatment concomitantly promotes loss of the recycling endosome-associated …
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Chemical Reporters for Bacterial Pathogenesis and Beyond
… modifications to explore their functions in bacterial pathogenesis as well as basic biological pathways. Initially, I developed amino acid chemical reporters that allow the cell-selective labeling of bacterial proteomes to facilitate the analysis of bacterial protein expression during …
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Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes
… structural and biochemical characterization of bacterial proteins involved in metabolite regulation and natural product biosynthesis. In the first case, I will describe the structural basis for transcriptional regulation of a small molecule metabolite, p-coumarate, and the remaining cases …
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CBK Bacteriophage Genome Annotation and Organization
… had significant similarity with the other bacterial and phage proteins, with 20 of them similar to other phage proteins. An additional 48 coding sequences were similar to hypothetical bacterial proteins. Also, 24 tRNAs were present in the genome.</p>
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Understanding the impact of Russian wheat aphid-associated microbes on plants
… This dissertation investigates the influence of bacterial communities associated with the Russian wheat aphid (RWA, Diuraphis noxia) on plant responses, focusing on barley and wheat as plant host systems. Through a combination of proteomics, microbiome analysis, transcriptomics and biological …
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Exploring the gut microbiome: metaproteome analysis of HIV-exposed uninfected infants
… (SWATH) to identify human and microbiome proteins in samples using Evosep40-SPD and TTOF 6600. In-silico library in DIA-NN used for protein identification, followed by data clean up and pre-processing in R studio and excel. Analysis of the proteins carried out in R studio and Metaboanalyst …
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Insights into the structures and dynamics of the pathogen secreted effectors AVR3A11 and tarp through the application of NMR spectroscopy
… their hosts at the molecular level. The class of bacterial proteins directly responsible for these inter-organism interactions have been termed e�ector proteins and can function in either an extracellular or, once secreted into the host cell, an intracellular environment. Primarily through the use …
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Purification and characterisation of novel recombinant β-glucosidases from aspergillus with application in biofuel production
… to fermentable sugars. A total of 166 proteins were identified as β-glucosidases after manual BLASTp search on the Aspergillus comparative database from eight species. Evidence for Horizontal Gene Transfer (HGT) of bacterial origin of some β-glucosidase genes was provided by their lack …
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New insights into a complex host-pathogen interaction: mechanisms of Helicobacter pylori activation of NF-κB
… The goal of this work is to better define the bacterial proteins and the cellular signaling molecules involved in H. pylori-induced NF-κB activation. The H. pylori virulence factor CagA, encoded by the cagPAI, is injected into host cells via a type 4 secretion system, where it interacts with a …
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A Proteomics Approach to Investigate Uropathogenic Escherichia coli
… classical shotgun proteomics approach to analyse proteins extracted from multiple strains of UPEC following growth in various environments (Lysogeny broth, artificial urine medium and co-culture with uroepithelial cells). The digested proteins and peptides from all fractions were separated on a …
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Variability of Cholesterol Accessibility in Human Red Blood Cells Measured Using a Bacterial Cholesterol Binding Toxin
… anthrolysin O, a cholesterol-binding bacterial toxin, to measure accessible cholesterol in human red blood cells (RBCs). Accessible cholesterol levels were stable within individuals, but varied >10 fold among individuals. Significant variation was observed among ethnic groups …
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Structural Basis for Signal Transduction in LOV Blue Light Photoreceptors
… biophysical and biochemical studies of LOV proteins containing point mutations at key residues, we determined that structural strain placed on the domain following light-induced covalent adduct formation regulates both structural based signal transduction as well as dark state recovery …
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Analysis of Bacterial-Host Interactions Between Campylobacter jejuni and the Avian Host During Commensalism
Campylobacter jejuni is a leading cause of bacterial enteritis in humans throughout the world. In contrast to the disease seen in humans upon infection, C. jejuni promotes an asymptomatic, intestinal colonization of many animals, especially avian species, to result in commensalism. The primary …
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Ion Selectivity and Gating in the NAK Channel
Ion selectivity and gating are two fundamental properties central to proper physiological functioning of ion channels. In this work, a thorough structural characterization of ion binding profiles is presented for the NaK channel, a non selective cation channel from Bacillus cereus, along with an …
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CcpA-Mediated Carbon Catabolite Repression of Virulence in the Group A Streptoccus
The group A streptococcus (GAS) is a strict human pathogen, which causes a broad spectrum of diseases ranging from the self-limiting diseases such as pharyngitis and impetigo to the more severe invasive disease such as necrotizing fasciitis. The coordinate expression of a wide array of virulence …
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Acyloxyacyl Hydrolase: Studies on Its Regulation and Function in Mus Musculus
… is an enzyme that detoxifies Gram-negative bacterial lipopolysaccharides (LPS) by selectively removing secondary acyl chains from the lipid A moiety. Originally found in neutrophils, it is also produced by monocyte-macrophages and renal proximal tubule cells. In the studies described here, I …
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Structural characterization of superbug proteins involved in regulating beta-lactam resistance
… thesis is the structural characterization of bacterial proteins involved in regulating β-lactam resistance. The notorious resistance of Staphylococcus aureus primarily stems from the production of β-lactamases and PBP2a, a low-affinity PBP which confers broad-spectrum β-lactam resistance in …
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High throughput RNA interference based screen for the identification of genes implicated in listeria monocytogenes infection
… has acquired a versatile arsenal of adaptor proteins. It uses these proteins to invade and undermine the host immune surveillance system, as well as to survive and propagate within the host cell. Our understanding of how L. monocytogenes infects cells points towards an interplay between …
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Structural and Kinetic Characterization of Protein Ampylation by VopS Fic Domain
The bacterial pathogen Vibrio parahaemolyticus manipulates host signaling pathways by injecting type III effectors into the cytoplasm of the target cell. One of these effectors, VopS, blocks actin assembly by AMPylating a conserved threonine residue in the switch 1 region of Rho GTPases. The …
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