Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 40 for “"Bacterial DNA"”.
-
Single-molecule studies of bacterial DNA replication and translesion synthesis
Faithful replication of genomic DNA is crucial for the survival of a cell. In order to achieve high-level accuracy in copying its genome, all cells employ replicative DNA polymerases that have intrinsic high fidelity. When an error occurs on the template DNA strand, in the form of lesions caused by …
-
Periodontal bacterial-DNA initiated immuno-inflammatory responses in human osteoblastic cells
… receptors including Toll like receptors (TLRs). Bacterial DNA (bDNA) is one of the PAMPs mainly recognized by TLR9. Interaction of bDNA and its receptors leads to activation of inflammatory signaling pathways potentially resulting in periodontal bone destruction. The aim of this study was to …
-
The identification and characterization of genomic bacterial DNA utilizing vibrational spectroscopy
… of pure intact genomic Deoxyribonucleic Acids (DNA) from two species of Bacillus bacteria complexed with Tris(hydroxmethyl)aminomethane (Tris) and Ethylendiaminetetraacetic Acid (EDTA)...a new methodology for the accurate differentiation and identification of bacterial genomic DNA has been …
-
Soil Microbial Community Distributions And Disease Suppressiveness In The Coastal Plain Of Georgia
… species diversity and quantities of microbial DNA were determined from each of these vegetation types at three separate locations near Statesboro, Georgia. Length heterogeneity PCR(LH-PCR) methods and subsequent analysis of fungal, bacterial, and metazoan communities by analysis of similarity …
-
The role of gut microbiota in systemic lupus erythematosus
… and progression. Finally, we demonstrated that bacterial DNA from the gut microbiota might be the inducer of Breg cells, as bacterial DNA administration at young age reproduced the beneficial effect seen in the Breg adoptive transfer experiment. Future studies are required to examine the …
-
DNA-DNA Checkerboard Hybridization to Identify Bacteria in Human Plaque Samples
<p>Previous research has shown that using DNA probes to identify the presence of specific bacteria in human plaque samples is superior to the commonly used anaerobic cultural procedures. Some periodontal pathogens are either uncultivable or very difficult to grow, such as<em> Prevotella intermedia, …
-
Development of A Molecular Gram-Stain Assay For The Diagnosis of Blood Stream Infections Associated With Sepsis
… analysis is the gold standard for diagnosing bacterial infections associated with sepsis. This method generates a high rate of false negative results and, in general, requires 20 to 48 hr to provide results. Both of these problems are related to the requirement that the bacterial pathogens …
-
Validation of a Pan-fungal polymerase chain reaction (PCR) assay for the detection and identification of medically important fungi in a diagnostic laboratory
… identified reference fungal isolates. The DNA yield and purity of three different DNA extraction methods was assessed, using 6 representative fungal isolates. The ZR Fungal/Bacterial DNA MicroPrep™ produced a median concentration of 17.28 ng/μl,which was significantly higher (p value = …
-
Elemental content to rice grain related to growing regions and soil microbiology
… concentrations in soil and porewater. Moreover, bacterial DNA was extracted from paddy soils and log2 copy number of 16S rRNA and arsM genes were detected using qPCR. Those results were linked with the soil chemical parameters. The results of this study suggested that edaphic factors such as pH …
-
Novel derivatives of nicotinamide adenine dinucleotide (NAD) and their biological evaluation against NAD-consuming enzymes
… us to probe the active site of an NAD+-dependent bacterial DNA ligase. The selective activity of 8-aryl/heteroaryl NAD+ derivatives against different NAD+-consuming enzymes offers excellent opportunities for their application as tool compounds in in-vitro/in-vivo studies, and as inhibitor …
-
Effects of 5��-Dihydrotestosterone (DHT) on Mouse Gut Microbiome– A Study of Sex Differences and Hormonal Effects on Gut Microbiome Composition
… changes in the gut microbiome. Extracted bacterial DNA from intestinal samples were subjected to 16S rRNA sequencing. Sequence reads were analyzed using MicrobiomeAnalyst and Piphillin<em>, </em>two web-based programs for phylogenetic and functional analysis. We identified a significant …
-
The cell-intrinsic innate immune response to DNA damage in human cells
The innate immune response has evolved to detect DNA from viruses and intracellular bacteria and differentiate this from our almost identical self-DNA. DNA detected in the cytoplasm is thought to be identified as foreign due to its location, and bacterial DNA in endosomes in recognised by its …
-
The application of molecular techniques for the rapid and sensitive detection of gastrointestinal pathogens directly in food
… shown to be robust and reliable for preparing bacterial DNA from food enrichment broths. The PCR assays and MagNA Pure™ was applied to enrichment broths inoculated with 558 naturally-contaminated food and environmental samples in a field trial. Concordance was found between PCR results and …
-
Mechanisms of anaerobic nitric oxide detoxification by Salmonella enterica serovar Typhimurium
… (ROS and RNS) are an integral part of this antibacterial defence of the immune system. Exposure to ROS and RNS occurs within phagocytic immune cells such as macrophages, where such generation of radicals is used to combat pathogens. NO is a radical belonging to the group of RNS that damages …
-
An integrative, community-collaborative approach to investigating health, diet, and the oral microbiome in ancestral and descendant coast Tsimshian communities
… of increasing social complexity. Using ancient bacterial DNA extracted from the dental calculus of the ancestral community, this project also characterizes the composition of the oral microbiome of this community, and examines variation in oral microbial diversity correlated with …
-
Nucleic acid modifications in bacterial pathogens - impact on pathogenesis, diagnosis, and therapy
… Given the enormous global disease burden due to bacterial infections, and the rapidly increasing rates of antibiotic resistance reported across the world, the need for research to address mechanisms of bacterial survival is more pressing than ever. The goal of this thesis was to determine the …
-
The house dust microbiota in the Drakenstein Child Health Study
… comprises many niches that are occupied by bacterial communities. The composition of these bacterial communities may be influenced by numerous factors such as number of occupants, pets, season and location. Understanding the house dust microbial community is vital to understanding its' …
-
Incorporation of CpG Oligodeoxynucleotides into α2-Macroglobulin: Development of a Novel Vaccine Adjuvant Delivery Mechanism
Bacterial DNA is immunostimulatory, and the motifs responsible for this activity are unmethylated CpG dinucleotides. Following cellular uptake, CpG-containing oligodeoxynucleotides (CpG ODN) are trafficked to the endosome where they bind Toll-like receptor 9 (TLR9) to initiate a signaling cascade …
-
Magnetic bead-based DNA extraction and purification microfluidic chip
A magnetic bead-based DNA extraction and purification device has been designed to be used for extraction of the target DNA molecules from whole blood sample. Mixing and separation steps are performed using functionalised superparamagnetic beads suspended in the cell lysis buffer in a circular …
-
Evaluation of the pharmacokinetics, pharmacodynamics, and safety of enrofloxacin administration to pregnant mares
… necessary in pregnant mares affected by severe bacterial infections such as cellulitis, bronchopneumonia, or placentitis. Despite the many antimicrobials available for human use, very few antimicrobials are cost-effective and approved for use in the horse. Enrofloxacin is a fluoroquinolone …
Page 1 of 2