Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Bacterial Artificial Chromosome"”.
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Klonierung und Mutagenese des Bovinen Herpesvirus Typ 1 als ein infektiöses künstliches bakterielles Chromosom (bacterial artificial chromosome, BAC)
… Schönböken genome was cloned as an infectious bacterial artificial chromosome (BAC), by inserting mini F plasmid sequences into the glycoprotein E (gE) open reading frame (ORF). DNA of the resulting BAC clone pBHV-1DgE was transfected into permissive bovine kidney cells and infectious BHV-1DgE …
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Functional genomics in the stroke-prone spontaneously hypertensive rat: genome wide and candidate gene analysis
… and functional genomic strategies investigated chromosome 2 candidate hypertension genes, focussing on the oxidative-stress defence gene, glutathione s-transferse mu type 1 (Gstm1). Ingenuity pathway analysis of renal microarrays in 5 and 16-week SHRSP, normotensive Wistar Kyoto (WKY) and …
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Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies
… kb using pulsed field gel electrophoresis. A bacterial artificial chromosome (BAC) was used to create a large insert library of the H. felis genomic DNA. Using hybridization experiments and fingerprinting analysis, these BACs were ordered into a genomic encyclopedia with a restriction map. The …
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Systematic Enhancement of Human Herpesvirus 6A Reconstitution: Bridging Technical Refinement and Host Determinants of Virus Replication
… efficient recombinant HHV-6A reconstitution from bacterial artificial chromosome (BAC) constructs, a process historically hampered by low transfection efficiencies and prolonged propagation duration. We demonstrated that pretreatment with dimethyl sulfoxide (DMSO) and exonuclease V (ExoV), coupled …
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Sequence characterization of the HSA12/22/12 evolutionary breakpoint region on SSC5q21
… map constructed by Meyers and colleagues. Bacterial artificial chromosome (BAC) fingerprint analysis and radiation hybrid mapping of BAC-end sequences was used to construct a minimum tiling path (MTP) encompassing each breakpoint region. The MTP clones were sequenced. The MTP sequences were …
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Synaptic Protein Profiling in the Mammalian Brain
… In order to achieve this, we have used the BAC (bacterial artificial chromosome) transgenic approach to target particular neurons for expression of a given neurotransmitter receptor fused to an affinity tag. Immunohistochemistry of fixed brain tissue confirmed the correct localization of each …
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Construction of a Simian Immunodeficiency Virus Vaccine from Cynomolgus Macaque Cytomegalovirus
… macaque cytomegalovirus was first cloned as a bacterial artificial chromosome (BAC) to facilitate the preservation of the virus without further accumulation of attenuations from in vitro passaging. After sequencing the BAC, and comparison of the growth kinetics of the viruses derived from the …
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Characterization and Development of a Novel Cytomegalovirus for Evaluation as an HIV Vaccine Viral Vector in a Non-Human Primate Model
… virus for vaccine purposes, I cloned CyCMV as a Bacterial Artificial Chromosome (BAC). The cloning of CyCMV-BAC enabled the virus to be transformed into a bacterial system for downstream insertion of vaccine antigens, as well as future viral manipulations, including attenuations. This newly …
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Spatial-Temporal Regulation of the Atonal Homolog 1 Gene
… by deleting different conserved regions in a bacterial artificial chromosome (318GFPBAC) containing ~180 kb of sequence 5' and 3' of atonal homolog 1 and assaying for green fluorescent protein expression in transgenic mice. Transgenic embryos were analyzed at embryonic day 10.5 in the neural …
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Characterization of malus germplasm and QTL mapping of fruit quality traits
… A total of 502 SSRs derived from three sources, bacterial artificial chromosome (BAC) end sequencing, expressed sequence tag (EST) sequences, and previously published markers, were used to genotype progeny. Linkage groups were mapped, and despite genotype by environment interactions and sometimes …
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A Molecular Cytogenetic Profile of the Chronic Myeloproliferative Disorders
… a recognisable pathogenomic abnormality, the Ph chromosome. The remaining disorders have as yet, no defined disease markers although up to 30% display recurrent cytogenetic aberrations. Historically, conventional cytogenetic methods have relied on culturing of bone marrow (BM) aspirates to obtain …
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Haplotype-Specific Effects of the Slam/Cd2 Family on the Immune Response
… a 900kb interval between 171.3 and 172.2Mb on chromosome 1. A Bacterial Artificial Chromosome (BACs) contig that spanned the interval was constructed, and a tiling pathway comprised of six BACs was sequenced. Sequence analysis revealed a dense region of 24 expressed genes. Expression studies …
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Identification of virulence determinants of Mycobacterium tuberculosis via genetic comparisons of a virulent and an attenuated strain of Mycobacterium tuberculosis.
… DNA technologies: two-dimensional bacterial genome display, and bacterial comparative genomic hybridisation. The two-dimensional technologies were optimised for mycobacterial use, but failed to yield reproducible genomic differences between the two strains. Expression differences …
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Characterization of chalcone synthase genes in red raspberry cultivar (rubus idaeus)
… the PKS/CHS gene regions within the three Bacterial Artificial Chromosome clones (BACs), which originated from the genome of the European red raspberries (Rubus idaeus) cv. Glen Moy (SCRI), using 454 sequencing technology assisted by a systematic construction of fosmid libraries. The two …
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Molecular genetics and functions of Kaposi's sarcoma-associated herpesvirus glycoproteins in viral entry and virus-induced cell fusion
… full-length KSHV genome has been cloned into a bacterial artificial chromosome (BAC) and successfully recovered in 293 cells. The herpesviral glycoproteins are structural components of the KSHV particle and are thought to facilitate virus entry, egress and virus-induced cell fusion. …
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QTL Mapping and Molecular Dissection of Meat Quality Traits in Swine
… map was used, together with porcine linkage and bacterial artificial chromosome (BAC) physical maps, to fine-map a QTL affecting pork tenderness that was detected, on the q arm of porcine chromosome 2 (SSC2q), within the Illinois Meat Quality Pedigree (IMQP). Nine novel microsatellite markers …
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Two Odorant-Binding Protein Genes in Mosquitoes: Comparative Genomics, Expression, and Function
… in Anopheles quadriannulatus by screening bacterial artificial chromosome (BAC) libraries of these species. Comparative analyses revealed several interesting features including segments of conserved non-coding sequences (CNSs) that contain potential regulatory elements relevant to olfactory …
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Tetracycline-dependent gene expression in cholinergic neurons of transgenic mice : a tool to study Neuregulin-1 function in vivo
… all required regulatory elements were present, a bacterial artificial chromosome (BAC), roughly 200kb in size, containing the ChAT gene and flanking sequences was used. Homologous recombination in bacteria was used to insert the tTA2S cDNA into the ChAT gene. The resulting ChAT-tTA2S BAC construct …
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Deconstructing G Protein-Coupled Receptor Dimer Pharmacology: Case Studies in Dopamine D1 and D2 Receptors
… spiny neurons. Although we found evidence using bacterial artificial chromosome-D1 and D2 reporter mice that D1 and D2 receptors are co-expressed in a small number of brain regions, we failed to observe D1-D2 receptor colocalization, suggesting the possibility that in neurons the receptors are …
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